Feasibility of implementing cell-based pathway reporter assays in early high-throughput screening assay cascades for antibody drug discovery

Journal of Biomolecular Screening

Volumn 17, Issue 6, 2012, Pages 713-726

  Smith, Alison J ^a   Hancock, Michael K ^b   Bi, Kun ^b   Andrews, John ^a   Harrison, Paula ^a   Vaughan, Tristan J ^a  

^a MEDIMMUNE   (United States)

^b Life Technologies   (United States)

Author keywords

antibody lead isolation; cell based assay tolerance; crude antibody samples; high throughput screening assays; reporter assays

Indexed keywords

ANTIBODY; BETA LACTAMASE; DOXYCYCLINE; GLUTAMINE; MITOMYCIN C; PENICILLIN G; STREPTOMYCIN;

ARTICLE; CONTROLLED STUDY; DRUG DEVELOPMENT; HIGH THROUGHPUT SCREENING; HYBRIDOMA; PHAGE DISPLAY; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; BETA-LACTAMASES; BIOLOGICAL ASSAY; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; DRUG DISCOVERY; GENES, REPORTER; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYBRIDOMAS; JURKAT CELLS; SIGNAL TRANSDUCTION;

EID: 84862168701     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112442962     Document Type: Article

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