Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Scott, Nathan ^c   Reynolds, Catherine B ^c   Wright, Michael J ^a,c   Qazi, Omar ^b,c   Fairweather, Neil ^c   Deonarain, Mahendra P ^c  

^a UCB PHARMA   (Belgium)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; ESCHERICHIA COLI; LIBRARIES; NUCLEIC ACIDS; ORGANIC ACIDS;

BACTERIAL INNER MEMBRANES; CODON BIAS; COMBINATORIAL LIBRARIES; CULTURE SUPERNATANT; CYTOSOLIC; DIFFERENTIAL DEGREES; DNA MOTIFS; E. COLI; EXPRESSION LEVELS; EXTRACELLULAR; HIGH YIELDS; LOCALISATION; LOW LEVELS; PERIPLASM; PHAGE DISPLAYS; POSITIVE CORRELATIONS; PROTEIN EXPRESSIONS; SEQUENCE HOMOLOGIES; SINGLE CHAINS; SINGLE-CHAIN FV; SOLUBLE PROTEINS; TETANUS TOXINS; TWO PARAMETERS;

THERMODYNAMIC STABILITY;

EPITOPE; MESSENGER RNA; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TETANUS TOXOID; PEPTIDE FRAGMENT; PEPTIDE LIBRARY; TETANUS TOXIN; TETANUS TOXIN FRAGMENT C;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL TRANSLOCATION; BACTERIUM CULTURE; BUDDING; CELL AGGREGATION; CODON USAGE; CORRELATIONAL STUDY; CYTOPLASM; DNA BINDING MOTIF; ESCHERICHIA COLI; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SEQUENCE HOMOLOGY; SUPERNATANT; AMINO ACID SEQUENCE; CHEMISTRY; COLIPHAGE; DENSITOMETRY; GENE EXPRESSION; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPHEROPLAST;

BACTERIA (MICROORGANISMS);

AMINO ACID SEQUENCE; COLIPHAGES; DENSITOMETRY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PEPTIDE LIBRARY; PERIPLASM; SPHEROPLASTS; TETANUS TOXIN;

EID: 59149105599     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-97     Document Type: Article

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