A simple high-throughput purification method for hit identification in protein screening

Journal of Immunological Methods

Volumn 339, Issue 1, 2008, Pages 38-46

  Cummins, Emma ^a   Luxenberg, Deborah P ^b   McAleese, Fionnuala ^b   Widom, Angela ^b   Fennell, Brian J ^a   Darmanin Sheehan, Alfredo ^a   Whitters, Matthew J ^b   Bloom, Laird ^b   Gill, Davinder ^b   Cunningham, Orla ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b PFIZER INC   (United States)

Author keywords

High throughput purification and screening; Protein purification; scFv

Indexed keywords

IMMUNOGLOBULIN FC FRAGMENT; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ARTICLE; BINDING ASSAY; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FILTER; INTERMETHOD COMPARISON; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ANIMALS; ANTIBODIES, MONOCLONAL; ESCHERICHIA COLI; FEMALE; HUMANS; IMMUNOGLOBULIN CONSTANT REGIONS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN VARIABLE REGION; MALE; PERIPLASM; PERIPLASMIC PROTEINS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 53049088558     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.07.016     Document Type: Article

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