Development of an intact cell reporter gene β-lactamase assay for G protein-coupled receptors for high-throughput screening

Analytical Biochemistry

Volumn 314, Issue 1, 2003, Pages 16-29

  Kunapuli, Priya ^a   Ransom, Richard ^a   Murphy, Kathy L ^a   Pettibone, Doug ^a   Kerby, Julie ^a   Grimwood, Sarah ^a,b   Zuck, Paul ^a   Hodder, Peter ^a   Lacson, Raul ^a   Hoffman, Ira ^a   Inglese, James ^a   Strulovici, Berta ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b PFIZER INC   (United States)

Author keywords

Bradykinin B1 receptor; GPCR; High throughput screening; Lactamase reporter

Indexed keywords

CLONE CELLS; GENES; MAMMALS; PROTEINS;

BRADYKININ B1 RECEPTOR; FLUOROGENIC SUBSTRATE; G PROTEIN COUPLED RECEPTORS; GPCR; HIGH THROUGHPUT SCREENING; LACTAMASES; PHARMACOLOGICAL CHARACTERIZATION; PHYSIOLOGICAL DISORDERS;

CYTOLOGY;

BETA LACTAMASE; BRADYKININ ANTAGONIST; BRADYKININ B1 RECEPTOR; BRADYKININ RECEPTOR AGONIST; G PROTEIN COUPLED RECEPTOR; LIGAND;

ANIMAL CELL; ARTICLE; BINDING ASSAY; CELL CLONING; CELL DENSITY; CELL LINE; CELL MEMBRANE PERMEABILITY; CELL STIMULATION; CHO CELL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GENETIC ANALYSIS; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REPORTER GENE; SCREENING;

MAMMALIA;

EID: 0037333313     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00587-0     Document Type: Article

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