A new mouse model for mania shares genetic correlates with human bipolar disorder

PLoS ONE

Volumn 7, Issue 6, 2012, Pages

  Saul, Michael C ^a   Gessay, Griffin M ^a   Gammie, Stephen C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ATTENTION DEFICIT DISORDER; BIPOLAR DISORDER; CAT GENE; CMKLR1 GENE; CONTROLLED STUDY; EPOR GENE; FHIT GENE; GENE; GENE CLUSTER; GENE EXPRESSION; GENE LOCUS; GENETIC ASSOCIATION; GENETIC LINKAGE; GENETIC TRANSCRIPTION; MANIA; MICROARRAY ANALYSIS; MOUSE; MOUSE STRAIN; NONHUMAN; NPSR1 GENE; NUCLEOTIDE SEQUENCE; P2RX7 GENE; PHENOTYPE; SCHIZOPHRENIA; SEQUENCE HOMOLOGY; SINGLE NUCLEOTIDE POLYMORPHISM; SMARCA4 GENE; TAC1 GENE; ANIMAL; ANIMAL BEHAVIOR; CHROMATIN; DISEASE MODEL; DNA MICROARRAY; GENE REGULATORY NETWORK; GENETIC PREDISPOSITION; GENETICS; HUMAN; HUMAN GENOME; METABOLISM; MOTOR ACTIVITY; PATHOPHYSIOLOGY; PHYSIOLOGY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

MAMMALIA; MUS; RODENTIA;

ANIMALS; BEHAVIOR, ANIMAL; BIPOLAR DISORDER; CHROMATIN; DISEASE MODELS, ANIMAL; GENE REGULATORY NETWORKS; GENETIC PREDISPOSITION TO DISEASE; GENOME, HUMAN; HUMANS; MICE; MOTOR ACTIVITY; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHENOTYPE; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84861899751     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038128     Document Type: Article

References (103)

1. Angst J, Gamma A, Benazzi F, Ajdacic V, Eich D, et al. (2003) Diagnostic issues in bipolar disorder. Eur Neuropsychopharmacol 13: S43-S50.
2. Kessler RC, Berglund P, Demler O, Jin R, Merikangas KR, et al. (2005) Lifetime prevalence and age-of-onset distributions of DSM-IV disorders in the national comorbidity survey replication. Arch Gen Psychiatry 62: 593-602.
3. Merikangas KR, Akiskal HS, Angst J, Greenberg PE, Hirschfeld RMA, et al. (2007) Lifetime and 12-month prevalence of bipolar spectrum disorder in the National Comorbidity Survey replication. Arch Gen Psychiatry 64: 543-552.
4. Smoller JW, Finn CT, (2003) Family, twin, and adoption studies of bipolar disorder. Am J Med Genet C 123C: 48-58.
5. Gershon ES, Alliey-Rodriguez N, Liu C, (2011) After GWAS: searching for genetic risk for schizophrenia and bipolar disorder. Am J Psychiatry 168: 253-256.
6. Le-Niculescu H, McFarland MJ, Mamidipalli S, Ogden CA, Kuczenski R, et al. (2007) Convergent Functional Genomics of bipolar disorder: From animal model pharmacogenomics to human genetics and biomarkers. Neurosci Biobehav Rev 31: 897-903.
7. Segurado R, Detera-Wadleigh SD, Levinson DF, Lewis CM, Gill M, et al. (2003) Genome scan meta-analysis of schizophrenia and bipolar disorder, part III: bipolar disorder. Am J Hum Genet 73: 49-62.
8. Serretti A, Mandelli L, (2008) The genetics of bipolar disorder: genome 'hot regions,' genes, new potential candidates and future directions. Mol Psychiatr 13: 742-771.
9. Rudan I, (2010) New technologies provide insights into genetic basis of psychiatric disorders and explain their co-morbidity. Psychiatr Danub 22: 190-192.
10. Crow TJ, (2007) How and why genetic linkage has not solved the problem of psychosis: review and hypothesis. Am J Psychiatry 164: 13-21.
11. Baum AE, Hamshere M, Green E, Cichon S, Rietschel M, et al. (2008) Meta-analysis of two genome-wide association studies of bipolar disorder reveals important points of agreement. Mol Psychiatr 13: 466-467.
12. Einat H, (2006) Modelling facets of mania - new directions related to the notion of endophenotypes. J Psychopharmacol 20: 714-722.
13. Furukawa T, Ushizima I, Ono N, (1975) Modifications by lithium of behavioral responses to methamphetamine and tetrabenazine. Psychopharmacology 42: 243-248.
14. Berggren U, Engel J, Liljequist S, (1981) The effect of lithium on the locomotor stimulation induced by dependence-producing drugs. J Neural Transm 50: 157-164.
15. Giros B, Jaber M, Jones SR, Wightman RM, Caron MG, (1996) Hyperlocomotion and indifference to cocaine and amphetamine in mice lacking the dopamine transporter. Nature 379: 606-612.
16. Rodriguiz RM, Chu R, Caron MG, Wetsel WC, (2004) Aberrant responses in social interaction of dopamine transporter knockout mice. Behav Brain Res 148: 185-198.
17. Roybal K, Theobold D, Graham A, DiNieri JA, Russo SJ, et al. (2007) Mania-like behavior induced by disruption of CLOCK. Proc Natl Acad Sci U S A 104: 6406-6411.
18. Colleen AM, (2007) Circadian genes, rhythms and the biology of mood disorders. Pharmacol Ther 114: 222-232.
19. Hannah-Poquette C, Anderson GW, Flaisher-Grinberg S, Wang J, Meinerding TM, et al. (2011) Modeling mania: Further validation for Black Swiss mice as model animals. Behav Brain Res 223: 222-226.
20. Flaisher-Grinberg S, Einat H, (2010) Strain-specific battery of tests for domains of mania: effects of valproate, lithium and imipramine. Front Psychiatr 1: 1-10.
21. Overstreet D, Pucilowski O, Rezvani A, Janowsky D, (1995) Administration of antidepressants, diazepam and psychomotor stimulants further confirms the utility of Flinders Sensitive Line rats as an animal model of depression. Psychopharmacology 121: 27-37.
22. Braw Y, Malkesman O, Merlender A, Bercovich A, Dagan M, et al. (2006) Stress hormones and emotion-regulation in two genetic animal models of depression. Psychoneuroendocrinology 31: 1105-1116.
23. Hasegawa S, Nishi K, Watanabe A, Overstreet DH, Diksic M, (2006) Brain 5-HT synthesis in the Flinders Sensitive Line rat model of depression: An autoradiographic study. Neurochem Int 48: 358-366.
24. Wegener G, Harvey BH, Bonefeld B, Müller HK, Volke V, et al. (2010) Increased stress-evoked nitric oxide signalling in the Flinders sensitive line (FSL) rat: a genetic animal model of depression. Int J Neuropsychopharmacol 13: 461-473.
25. Harrison-Read P, (2009) Models of mania and antimanic drug actions: progressing the endophenotype approach. J Psychopharmacol 23: 334-337.
26. Scotti M-AL, Lee G, Stevenson SA, Ostromecki AM, Wied TJ, et al. (2011) Behavioral and pharmacological assessment of a potential new mouse model for mania. Physiol Behav 103: 376-383.
27. Flaisher-Grinberg S, Kronfeld-Schor N, Einat H, (2010) Models of mania: from facets to domains and from animal models to model animals. J Psychopharmacol 24: 437-438.
28. Bertolino A, Frye M, Callicott JH, Mattay VS, Rakow R, et al. (2003) Neuronal pathology in the hippocampal area of patients with bipolar disorder: a study with proton magnetic resonance spectroscopic imaging. Biol Psychiat 53: 906-913.
29. Frey BN, Andreazza AC, Nery FG, Martins MR, Quevedo J, et al. (2007) The role of hippocampus in the pathophysiology of bipolar disorder. Behav Pharmacol 18: 419-430 410.1097/FBP.1090b1013e3282df1093cde.
30. Konradi C, Eaton M, MacDonald ML, Walsh J, Benes FM, et al. (2004) Molecular evidence for mitochondrial dysfunction in bipolar disorder. Arch Gen Psychiatry 61: 300-308.
31. Fatemi SH, Earle JA, McMenomy T, (2000) Reduction in Reelin immunoreactivity in hippocampus of subjects with schizophrenia, bipolar disorder and major depression. Mol Psychiatr 5: 654-663, 571.
32. Edgar PF, Douglas JE, Cooper GJ, Dean B, Kydd R, et al. (2000) Comparative proteome analysis of the hippocampus implicates chromosome 6 q in schizophrenia. Mol Psychiatr 5: 85-90.
33. Maes M, Scharpé S, Van Grootel L, Uyttenbroeck W, Cooreman W, et al. (1992) Higher α1-antitrypsin, haptoglobin, ceruloplasmin and lower retinol binding protein plasma levels during depression: Further evidence for the existence of an inflammatory response during that illness. J Affect Disord 24: 183-192.
34. Virit O, Altinda A, Selek S, Yumru M, Bulut M, et al. (2008) Increased Plasma Ceruloplasmin Levels in Schizophrenia. Bulletin of Clinical Psychopharmacology 18: 282-287.
35. Bowman MB, Lewis MS, (1982) The copper hypothesis of schizophrenia: A review. Neurosci Biobehav Rev 6: 321-328.
36. Miskowiak K, Vinberg M, Harmer C, Ehrenreich H, Kessing L, (2012) Erythropoietin: a candidate treatment for mood symptoms and memory dysfunction in depression. Psychopharmacology 219: 687-698.
37. Ehrenreich H, Degner D, Meller J, Brines M, Behe M, et al. (2003) Erythropoietin: a candidate compound for neuroprotection in schizophrenia. Mol Psychiatr 9: 42-54.
38. Miskowiak K, Favaron E, Hafizi S, Inkster B, Goodwin G, et al. (2009) Effects of erythropoietin on emotional processing biases in patients with major depression: an exploratory fMRI study. Psychopharmacology 207: 133-142.
39. Belmonte Mahon P, Pirooznia M, Goes FS, Seifuddin F, Steele J, et al. (2011) Genome-wide association analysis of age at onset and psychotic symptoms in bipolar disorder. Am J Med Genet B 156: 370-378.
40. Zubenko GS, Maher B, Hughes HB, Zubenko WN, Stiffler JS, et al. (2003) Genome-wide linkage survey for genetic loci that influence the development of depressive disorders in families with recurrent, early-onset, major depression. Am J Med Genet B 123B: 1-18.
41. Ogden CA, Rich ME, Schork NJ, Paulus MP, Geyer MA, et al. (2004) Candidate genes, pathways and mechanisms for bipolar (manic-depressive) and related disorders: an expanded convergent functional genomics approach. Mol Psychiatr 9: 1007-1029.
42. Berrettini WH, Rubinow DR, Nurnberger JI Jr, Simmons-Alling S, Post RM, et al. (1985) CSF substance P immunoreactivity in affective disorders. Biol Psychiat 20: 965-970.
43. Bondy B, Baghai TC, Minov C, Schüle C, Schwarz MJ, et al. (2003) Substance P serum levels are increased in major depression: preliminary results. Biol Psychiat 53: 538-542.
44. Barden N, Harvey M, Gagné B, Shink E, Tremblay M, et al. (2006) Analysis of single nucleotide polymorphisms in genes in the chromosome 12Q24.31 region points to P2RX7 as a susceptibility gene to bipolar affective disorder. Am J Med Genet B 141B: 374-382.
45. McQuillin A, Bass NJ, Choudhury K, Puri V, Kosmin M, et al. (2008) Case-control studies show that a non-conservative amino-acid change from a glutamine to arginine in the P2RX7 purinergic receptor protein is associated with both bipolar- and unipolar-affective disorders. Mol Psychiatr 14: 614-620.
46. Buttenschøn HN, Foldager L, Flint TJ, Olsen IM, Deleuran T, et al. (2010) Support for a bipolar affective disorder susceptibility locus on chromosome 12q24.3. Psychiatr Genet 20: 93-101.
47. Hejjas K, Szekely A, Domotor E, Halmai Z, Balogh G, et al. (2009) Association between depression and the Gln460Arg polymorphism of P2RX7 Gene: A dimensional approach. Am J Med Genet B 150B: 295-299.
48. Lucae S, Salyakina D, Barden N, Harvey M, Gagné B, et al. (2006) P2RX7, a gene coding for a purinergic ligand-gated ion channel, is associated with major depressive disorder. Hum Mol Genet 15: 2438-2445.
49. Nagy G, Ronai Z, Somogyi A, Sasvari-Szekely M, Rahman OA, et al. (2008) P2RX7 Gln460Arg polymorphism is associated with depression among diabetic patients. Prog Neuropsychopharmacol Biol Psychiatry 32: 1884-1888.
50. Lasky-Su J, Neale BM, Franke B, Anney RJL, Zhou K, et al. (2008) Genome-wide association scan of quantitative traits for attention deficit hyperactivity disorder identifies novel associations and confirms candidate gene associations. Am J Med Genet B 147B: 1345-1354.
51. McCarthy MJ, Nievergelt CM, Kelsoe JR, Welsh DK, (2012) A Survey of Genomic Studies Supports Association of Circadian Clock Genes with Bipolar Disorder Spectrum Illnesses and Lithium Response. PLoS ONE 7: e32091.
52. Frey BN, Andreazza AC, Kunz M, Gomes FA, Quevedo J, et al. (2007) Increased oxidative stress and DNA damage in bipolar disorder: A twin-case report. Prog Neuropsychopharmacol Biol Psychiatry 31: 283-285.
53. Andreazza AC, Cassini C, Rosa AR, Leite MC, de Almeida LMV, et al. (2007) Serum S100B and antioxidant enzymes in bipolar patients. J Psychiatr Res 41: 523-529.
54. Detera-Wadleigh SD, Akula N, (2011) A systems approach to the biology of mood disorders through network analysis of candidate genes. Pharmacopsychiatry 44: S35-S42.
55. Regland B, Johansson BV, Gottfries CG, (1994) Homocysteinemia and schizophrenia as a case of methylation deficiency. J Neural Transm 98: 143-152.
56. Won H, Mah W, Kim E, Kim J-W, Hahm E-K, et al. (2011) GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice. Nat Med 17: 566-572.
57. Middleton FA, Pato CN, Gentile KL, McGann L, Brown AM, et al. (2005) Gene expression analysis of peripheral blood leukocytes from discordant sib-pairs with schizophrenia and bipolar disorder reveals points of convergence between genetic and functional genomic approaches. Am J Med Genet B 136B: 12-25.
58. Reinscheid RK, Xu Y-L, (2005) Neuropeptide S as a novel arousal promoting peptide transmitter. FEBS Journal 272: 5689-5693.
59. Reinscheid RK, Xu YL, Civelli O, (2005) Neuropeptide S: a new player in the modulation of arousal and anxiety. Mol Interv 5: 42-46.
60. Schumacher J, Kaneva R, Jamra RA, Diaz GO, Ohlraun S, et al. (2005) Genomewide scan and fine-mapping linkage studies in four european samples with bipolar affective disorder suggest a new susceptibility locus on chromosome 1p35-p36 and provides further evidence of loci on chromosome 4q31 and 6q24. Am J Hum Genet 77: 1102-1111.
61. Curtis D, Kalsi G, Brynjolfsson J, McInnis M, O'Neill J, et al. (2003) Genome scan of pedigrees multiply affected with bipolar disorder provides further support for the presence of a susceptibility locus on chromosome 12q23-q24, and suggests the presence of additional loci on 1p and 1q. Psychiatr Genet 13: 77-84.
62. Kandaswamy R, McQuillin A, Curtis D, Gurling H, (2012) Tests of linkage and allelic association between markers in the 1p36 PRKCZ (Protein Kinase C Zeta) gene region and bipolar affective disorder. Am J Med Genet B 159B: 201-209.
63. Detera-Wadleigh SD, Barden N, Craddock N, Ewald H, Foroud T, et al. (1999) Chromosomes 12 and 16 workshop. Am J Med Genet 88: 255-259.
64. Ewald H, Flint T, Kruse TA, Mors O, (2002) A genome-wide scan shows significant linkage between bipolar disorder and chromosome 12q24.3 and suggestive linkage to chromosomes 1p22-21, 4p16, 6q14-22, 10q26 and 16p13.3. Mol Psychiatr 7: 734-744.
65. Ewald H, Degn B, Mors O, Kruse TA, (1998) Significant linkage between bipolar affective disorder and chromosome 12q24. Psychiatr Genet 8: 131-140.
66. McInnis MG, Lan TH, Willour VL, McMahon FJ, Simpson SG, et al. (2003) Genome-wide scan of bipolar disorder in 65 pedigrees: supportive evidence for linkage at 8q24, 18q22, 4q32, 2p12, and 13q12. Mol Psychiatr 8: 288-298.
67. Lyons-Warren A, Chang JJ, Balkissoon R, Kamiya A, Garant M, et al. (2005) Evidence of association between bipolar disorder and Citron on chromosome 12q24. Mol Psychiatr 10: 807-809.
68. Abou Jamra R, Fuerst R, Kaneva R, Orozco Diaz G, Rivas F, et al. (2007) The first genomewide interaction and locus-heterogeneity linkage scan in bipolar affective disorder: strong evidence of epistatic effects between loci on chromosomes 2q and 6q. Am J Hum Genet 81: 974-986.
69. Cheng R, Juo SH, Loth JE, Nee J, Iossifov I, et al. (2006) Genome-wide linkage scan in a large bipolar disorder sample from the National Institute of Mental Health genetics initiative suggests putative loci for bipolar disorder, psychosis, suicide, and panic disorder. Mol Psychiatr 11: 252-260.
70. Zandi PP, Badner JA, Steele J, Willour VL, Miao K, et al. (2007) Genome-wide linkage scan of 98 bipolar pedigrees and analysis of clinical covariates. Mol Psychiatr 12: 630-639.
71. Dick DM, Foroud T, Flury L, Bowman ES, Miller MJ, et al. (2003) Genomewide Linkage Analyses of Bipolar Disorder: A New Sample of 250 Pedigrees from the National Institute of Mental Health Genetics Initiative. The American Journal of Human Genetics 73: 107-114.
72. Schulze TG, Buervenich S, Badner JA, Steele CJM, Detera-Wadleigh SD, et al. (2004) Loci on chromosomes 6q and 6p interact to increase susceptibility to bipolar affective disorder in the national institute of mental health genetics initiative pedigrees. Biol Psychiat 56: 18-23.
73. Tomàs C, Cañellas F, Rodríguez V, Picornell A, Lafau O, et al. (2006) Genetic linkage study for bipolar disorders on chromosomes 17 and 18 in families with a high expression of mental illness from the Balearic Islands. Psychiatr Genet 16: 145-151.
74. Ewald H, Wikman FP, Teruel BM, Buttenschön HN, Torralba M, et al. (2005) A genome-wide search for risk genes using homozygosity mapping and microarrays with 1,494 single-nucleotide polymorphisms in 22 eastern Cuban families with bipolar disorder. Am J Med Genet B 133B: 25-30.
75. Doyle AE, Biederman J, Ferreira MAR, Wong P, Smoller JW, et al. (2010) Suggestive linkage of the child behavior checklist juvenile bipolar disorder phenotype to 1p21, 6p21, and 8q21. J Am Acad Child Adolesc Psychiatry 49: 378-387.
76. Etain B, Mathieu F, Rietschel M, Maier W, Albus M, et al. (2006) Genome-wide scan for genes involved in bipolar affective disorder in 70 European families ascertained through a bipolar type I early-onset proband: supportive evidence for linkage at 3p14. Mol Psychiatr 11: 685-694.
77. Marcheco-Teruel B, Flint TJ, Wikman FP, Torralbas M, González L, et al. (2006) A genome-wide linkage search for bipolar disorder susceptibility loci in a large and complex pedigree from the eastern part of Cuba. Am J Med Genet B 141B: 833-843.
78. Schosser A, Fuchs K, Leisch F, Bailer U, Meszaros K, et al. (2004) Possible linkage of schizophrenia and bipolar affective disorder to chromosome 3q29: a follow-up. J Psychiatr Res 38: 357-364.
79. McInnes LA, Escamilla MA, Service SK, Reus VI, Leon P, et al. (1996) A complete genome screen for genes predisposing to severe bipolar disorder in two Costa Rican pedigrees. Proc Natl Acad Sci U S A 93: 13060-13065.
80. Sklar P, Gabriel SB, McInnis MG, Bennett P, Lim YM, et al. (2002) Family-based association study of 76 candidate genes in bipolar disorder: BDNF is a potential risk locus. Brain-derived neutrophic factor. Mol Psychiatr 7: 579-593.
81. Neves-Pereira M, Mundo E, Muglia P, King N, Macciardi F, et al. (2002) The brain-derived neurotrophic factor gene confers susceptibility to bipolar disorder: evidence from a family-based association study. Am J Hum Genet 71: 651-655.
82. Fullerton JM, Donald JA, Mitchell PB, Schofield PR, (2010) Two-dimensional genome scan identifies multiple genetic interactions in bipolar affective disorder. Biol Psychiat 67: 478-486.
83. Pato CN, Pato MT, Kirby A, Petryshen TL, Medeiros H, et al. (2004) Genome-wide scan in Portuguese Island families implicates multiple loci in bipolar disorder: Fine mapping adds support on chromosomes 6 and 11. Am J Med Genet B 127B: 30-34.
84. Badenhop RF, Moses MJ, Scimone A, Mitchell PB, Ewen-White KR, et al. (2002) A genome screen of 13 bipolar affective disorder pedigrees provides evidence for susceptibility loci on chromosome 3 as well as chromosomes 9, 13 and 19. Mol Psychiatr 7: 851-859.
85. Nievergelt CM, Kripke DF, Barrett TB, Burg E, Remick RA, et al. (2006) Suggestive evidence for association of the circadian genes PERIOD3 and ARNTL with bipolar disorder. Am J Med Genet B 141B: 234-241.
86. Lien Y-J, Tsuang H-C, Chiang A, Liu C-M, Hsieh MH, et al. (2010) The multidimensionality of schizotypy in nonpsychotic relatives of patients with schizophrenia and its applications in ordered subsets linkage analysis of schizophrenia. Am J Med Genet B 153B: 1-9.
87. Romanos M, Freitag C, Jacob C, Craig DW, Dempfle A, et al. (2008) Genome-wide linkage analysis of ADHD using high-density SNP arrays: novel loci at 5q13.1 and 14q12. Mol Psychiatr 13: 522-530.
88. Swallow JG, Carter PA, Garland T, (1998) Artificial selection for increased wheel-running behavior in house mice. Behav Genet 28: 227-237.
89. Rhodes JS, Garland T Jr, Gammie SC, (2003) Patterns of brain activity associated with variation in voluntary wheel-running behavior. Behav Neurosci 117: 1243-1256.
90. Bronikowski AM, Rhodes JS, Garland T, Prolla TA, Awad TA, et al. (2004) The evolution of gene expression in mouse hippocampus in response to selective breeding for increased locomotor activity. Evolution 58: 2079-2086.
91. Gammie SC, Hasen NS, Rhodes JS, Girard I, Garland T Jr, (2003) Predatory aggression, but not maternal or intermale aggression, is associated with high voluntary wheel-running behavior in mice. Horm Behav 44: 209-221.
92. Consortium MGS, (2002) Initial sequencing and comparative analysis of the mouse genome. Nature 420: 520-562.
93. Gammie SC, Edelmann MN, Mandel-Brehm C, D'Anna KL, Auger AP, et al. (2008) Altered dopamine signaling in naturally occurring maternal neglect. PLoS ONE 3: e1974.
94. Edgar R, Domrachev M, Lash AE, (2002) Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res 30: 207-210.
95. Smyth G, (2005) limma: Linear models for microarray data. In: Gentleman R, Carey VJ, Huber W, Irizarry RA, Dudoit S, editors. Bioinformatics and Computational Biology Solutions Using R and Bioconductor: Springer New York pp. 397-420.
96. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, et al. (2009) The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55: 611-622.
97. Bonefeld BE, Elfving B, Wegener G, (2008) Reference genes for normalization: A study of rat brain tissue. Synapse 62: 302-309.
98. Gubern C, Hurtado O, Rodriguez R, Morales J, Romera V, et al. (2009) Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia. BMC Mol Biol 10: 57.
99. Pfaffl MW, Horgan GW, Dempfle L, (2002) Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30: e36.
100. Huang DW, Sherman BT, Lempicki RA, (2009) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res 37: 1-13.
101. Huang DW, Sherman BT, Lempicki RA, (2008) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 4: 44-57.
102. Gao J, Ade AS, Tarcea VG, Weymouth TE, Mirel BR, et al. (2009) Integrating and annotating the interactome using the MiMI plugin for cytoscape. Bioinformatics 25: 137-138.
103. Su G, Kuchinsky A, Morris JH, States DJ, Meng F, (2010) GLay: community structure analysis of biological networks. Bioinformatics 26: 3135-3137.