Ablation of Pparg2 impairs lipolysis and reveals murine strain differences in lipolytic responses

FASEB Journal

Volumn 26, Issue 5, 2012, Pages 1835-1844

  Rodriguez Cuenca, Sergio ^a   Carobbio, Stefania ^a   Vidal Puig, Antonio ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Adipose tissue; Insulin resistance

Indexed keywords

BETA 3 ADRENERGIC RECEPTOR; CATECHOLAMINE DERIVATIVE; INSULIN; MESSENGER RNA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA 2; TRIACYLGLYCEROL LIPASE;

ADIPOCYTE; ADIPOSE TISSUE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; FEMALE; GENE; GENE EXPRESSION; KNOCKOUT MOUSE; LIPID METABOLISM; LIPID TRANSPORT; LIPOGENESIS; LIPOLYSIS; METABOLIC SYNDROME X; MOUSE; MOUSE STRAIN; NONHUMAN; PHENOTYPE; PPARG2 GENE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; STRAIN DIFFERENCE; WILD TYPE;

ANIMALS; BLOTTING, WESTERN; CATECHOLAMINES; DOWN-REGULATION; LIPOLYSIS; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; PPAR GAMMA; REAL-TIME POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY;

MURINAE; MUS;

EID: 84860895270     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.11-193631     Document Type: Article

References (25)

1. Gray, S. L., and Vidal-Puig, A. J. (2007) Adipose tissue expandability in the maintenance of metabolic homeostasis. Nutr. Rev. 65, S7-S12
2. Medina-Gomez, G., Virtue, S., Lelliott, C., Boiani, R., Campbell, M., Christodoulides, C., Perrin, C., Jimenez-Linan, M., Blount, M., Dixon, J., Zahn, D., Thresher, R. R., Aparicio, S., Carlton, M., Colledge, W. H., Kettunen, M. I., Seppanen-Laakso, T., Sethi, J. K., O'Rahilly, S., Brindle, K., Cinti, S., Oresic, M., Burcelin, R., and Vidal-Puig, A. (2005) The link between nutritional status and insulin sensitivity is dependent on the adipocyte-specific peroxisome proliferator-activated receptor-gamma2 isoform. Diabetes 54, 1706-1716 (Pubitemid 40770761)
3. Langin, D. (2006) Control of fatty acid and glycerol release in adipose tissue lipolysis. Curr. Rev. Biol. 329, 598-607; discussion 653-595
4. Dalen, K. T., Schoonjans, K., Ulven, S. M., Weedon-Fekjaer, M. S., Bentzen, T. G., Koutnikova, H., Auwerx, J., and Nebb, H. I. (2004) Adipose tissue expression of the lipid droplet-associating proteins S3-12 and perilipin is controlled by peroxisome proliferator-activated receptor-gamma. Diabetes 53, 1243-1252 (Pubitemid 38569010)
5. Deng, T., Shan, S., Li, P. P., Shen, Z. F., Lu, X. P., Cheng, J., and Ning, Z. Q. (2006) Peroxisome proliferator-activated receptorgamma transcriptionally up-regulates hormone-sensitive lipase via the involvement of specificity protein-1. Endocrinology 147, 875-884 (Pubitemid 43113005)
6. Kershaw, E. E., Schupp, M., Guan, H. P., Gardner, N. P., Lazar, M. A., and Flier, J. S. (2007) PPARgamma regulates adipose triglyceride lipase in adipocytes in vitro and in vivo. Am. J. Physiol. Endocrinol. Metab. 293, E1736-E1745 (Pubitemid 350255127)
7. Kim, J. Y., Tillison, K., Lee, J. H., Rearick, D. A., and Smas, C. M. (2006) The adipose tissue triglyceride lipase ATGL/PNPLA2 is downregulated by insulin and TNF-alpha in 3T3-L1 adipocytes and is a target for transactivation by PPARgamma. Am. J. Physiol. Endocrinol. Metab. 291, E115-E127 (Pubitemid 44035316)
8. Festuccia, W. T., Laplante, M., Berthiaume, M., Gelinas, Y., and Deshaies, Y. (2006) PPARgamma agonism increases rat adipose tissue lipolysis, expression of glyceride lipases, and the response of lipolysis to hormonal control. Diabetologia 49, 2427-2436 (Pubitemid 44359105)
9. Jaworski, K., Sarkadi-Nagy, E., Duncan, R. E., Ahmadian, M., and Sul, H. S. (2007) Regulation of triglyceride metabolism. IV. Hormonal regulation of lipolysis in adipose tissue. Am. J. Physiol. Gastrointest. Liver Physiol. 293, G1-G4 (Pubitemid 47105157)
10. Rodbell, M. (1964) Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem. 239, 375-380
11. Pfaffl, M. W., Tichopad, A., Prgomet, C., and Neuvians, T. P. (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper- Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26, 509-515 (Pubitemid 38448421)
12. Zhang, J., Fu, M., Cui, T., Xiong, C., Xu, K., Zhong, W., Xiao, Y., Floyd, D., Liang, J., Li, E., Song, Q., and Chen, Y. E. (2004) Selective disruption of PPARgamma 2 impairs the development of adipose tissue and insulin sensitivity. Proc. Natl. Acad. Sci. U. S. A. 101, 10703-10708 (Pubitemid 38955806)
13. Vidal-Puig, A., Jimenez-Linan, M., Lowell, B. B., Hamann, A., Hu, E., Spiegelman, B., Flier, J. S., and Moller, D. E. (1996) Regulation of PPAR gamma gene expression by nutrition and obesity in rodents. J. Clin. Invest. 97, 2553-2561 (Pubitemid 26187343)
14. Harris, C. A., Haas, J. T., Streeper, R. S., Stone, S. J., Kumari, M., Yang, K., Han, X., Brownell, N., Gross, R. W., Zechner, R., and Farese, R. V., Jr. DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes. J. Lipid Res. 52, 657-667
15. Thiele, C., and Spandl, J. (2008) Cell biology of lipid droplets. Curr. Opinion Cell Biol. 20, 378-385
16. Snyder, P. B., Esselstyn, J. M., Loughney, K., Wolda, S. L., and Florio, V. A. (2005) The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis. J. Lipid Res. 46, 494-503 (Pubitemid 43109786)
17. Almind, K., and Kahn, C. R. (2004) Genetic Determinants of energy expenditure and insulin resistance in diet-induced obesity in mice. Diabetes 53, 3274-3285 (Pubitemid 39571714)
18. Puri, V., Ranjit, S., Konda, S., Nicoloro, S. M. C., Straubhaar, J., Chawla, A., Chouinard, M., Lin, C., Burkart, A., Corvera, S., Perugini, R. A., and Czech, M. P. (2008) Cidea is associated with lipid droplets and insulin sensitivity in humans. Proc. Natl. Acad. Sci. U. S. A. 105, 7833-7838 (Pubitemid 351872724)
19. Brasaemle, D. L. (2007) Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis. J. Lipid Res. 48, 2547-2559
20. Ducharme, N. A., and Bickel, P. E. (2008) Lipid droplets in lipogenesis and lipolysis. Endocrinology 149, 942-949
21. Granneman, J. G., Moore, H. P., Krishnamoorthy, R., and Rathod, M. (2009) Perilipin controls lipolysis by regulating the interactions of AB-hydrolase containing 5 (Abhd5) and adipose triglyceride lipase (Atgl). J. Biol. Chem. 284, 34538-34544
22. Tansey, J. T., Sztalryd, C., Gruia-Gray, J., Roush, D. L., Zee, J. V., Gavrilova, O., Reitman, M. L., Deng, C. X., Li, C., Kimmel, A. R., and Londos, C. (2001) Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity. Proc. Natl. Acad. Sci. U. S. A. 98, 6494-6499 (Pubitemid 32488265)
23. Cohen, A. W., Razani, B., Schubert, W., Williams, T. M., Wang, X. B., Iyengar, P., Brasaemle, D. L., Scherer, P. E., and Lisanti, M. P. (2004) Role of caveolin-1 in the modulation of lipolysis and lipid droplet formation. Diabetes 53, 1261-1270 (Pubitemid 38569012)
24. Ahmad, F., Lindh, R., Tang, Y., Ruishalme, I., Ost, A., Sahachartsiri, B., Stralfors, P., Degerman, E., and Manganiello, V. C. (2009) Differential regulation of adipocyte PDE3B in distinct membrane compartments by insulin and the beta3-adrenergic receptor agonist CL316243: effects of caveolin-1 knockdown on formation/maintenance of macromolecular signalling complexes. Biochem. J. 424, 399-410
25. Cohen, A. W., Razani, B., Wang, X. B., Combs, T. P., Williams, T. M., Scherer, P. E., and Lisanti, M. P. (2003) Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue. Am. J. Physiol. Cell Physiol. 285, C222-C235 (Pubitemid 36736354)