Comparison between two standardized cultural methods and 24 hour Duplex Sybr Green Real-Time PCR assay for Salmonella detection in meat samples

New Microbiologica

Volumn 34, Issue 3, 2011, Pages 299-305

  Delibato, Elisabetta ^a   Fiore, Alfonsina ^a   Anniballi, Fabrizio ^a   Auricchio, Bruna ^a   Filetici, Emma ^a   Orefice, Leucio ^a   Losio, Marina Nadia ^b   De Medici, Dario ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b Istituto Zooprofdattico della Lombardia ed Emilia Romagna   (Italy)

Author keywords

Rapid method; Real time PCR; Salmonella; Sybr Green

Indexed keywords

ARTICLE; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; MEAT; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SALMONELLA;

BACTERIOLOGICAL TECHNIQUES; FOOD MICROBIOLOGY; MEAT; POLYMERASE CHAIN REACTION; SALMONELLA;

SALMONELLA;

EID: 84860393731     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Abdulmawjood A., Roth S., Bulte M. (2002). Two methods for construction of internal amplification controls for the detection of Escherichia coli 0157 by polymerase chain reaction. Mol. Cell. Probes. 16, 335-339. (Pubitemid 35397436)
2. Bhunia A.K. (2008). Biosensors and bio-based methods for the separation and detection of foodborne pathogens. Adv. Food Nutr. Res. 54, 1-44.
3. De Medici D., Croci L., Delibato E, Di Pasquale S., Filetici E., Toti L. (2003). Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry. Appl. Environ. Microbiol. 69, 3456-3461. (Pubitemid 36693354)
4. EFSA. (2009). Trends and Sources of Zoonoses and Zoonotic Agents in the European Union in 2007. The EFSA Journal. 223.
5. Elizaquivel P., Aznar R. (2008). A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli 0157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables. Food Microbiol. 25, 705-713.
6. Fenicia L., Anniballi F., De Medici D., Delibato E, Aureli P. (2007). SYBR green real-time PCR method to detect Clostridium botulinum type A. Appl. Environ. Microbiol. 73, 2891-2896. (Pubitemid 46718571)
7. Fricker M., Messelhausser U., Busch U., Scherer S., Ehung-Schulz M. (2007). Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks. Appl. Environ. Microbiol. 73, 1892-1898. (Pubitemid 46449072)
8. FSIS, Ed. (2008). Isolation and Identification of Salmonella from Meat, Poultry, and Egg Products (MLG 4.04). Laboratory Guidebook. Edited by U.S.D. o. A.-F.S. a. I. Service. Athens, GA, USA: United States Department of Agriculture - Food Safety and Inspection Service.
9. Fukushima H., and Tsunomori Y. (2005). [Study of realtime PCR assays for rapid detection of food-borne pathogens]. Kansenshogaku Zasshi. 79, 644-655.
10. Gomez T.M., Motarjemi Y, Miyagawa S., Kaferstein F.K., Stohr K. (1997). Foodborne salmonellosis. World Health Stat Q. 50, 81-89. (Pubitemid 27359976)
11. Hoorfar J., Cook N., Malorny B., Wagner M., De Medici D., Abdulmawjood A., Fach P. (2004). Diagnostic PCR: making internal amplification control mandatory. J. Appl. Microbiol. 96, 221-222. (Pubitemid 38223547)
12. ISO, Ed. (2002). International Standard ISO/FDIS 6579. Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. Edited by I.O.f. Standardization. Geneva. Switzerland.
13. Klein D., Janda P., Steinborn R., Muller M., Salmons B., Gunzburg W.H. (1999). Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification. Electrophoresis. 20, 291-299. (Pubitemid 29125016)
14. Lofstrom C., Krause M., Josefsen M.H., Hansen F., Hoorfar J. (2009). Validation of a same-day realtime PCR method for screening of meat and carcass swabs for Salmonella. BMC Microbiol. 9, 85.
15. Malorny B., Cook N., D'Agostino M., De Medici D., Croci L., Abdulmawjood A., Fach P., Karpiskova R., Aymerich T., Kwaitek K. and Hoorfar J. (2004a). Multicenter validation of PCR-based method for detection of Salmonella in chicken and pig samples. J. AO AC Int. 87, 861-866.
16. Malorny B., Hoorfar J., Hugas M., Heuvelink A., Fach P., Ellerbroek L., Bunge C., Dorn C., Helmuth R. (2003). Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method. Int. J. Food Microbiol. 89, 241-249. (Pubitemid 37412698)
17. Malorny B., Paccassoni E., Fach P., Bunge C., Martin A., Helmuth R. (2004b). Diagnostic real-time PCR for detection of Salmonella in food. Appl. Environ. Microbiol. 70, 7046-7052. (Pubitemid 39643767)
18. Piknova L., Kaclikova E., Pangallo D., Polek B., Kuchta T. (2005). Quantification of Salmonella by 5'-nuclease real-time polymerase chain reaction targeted to fimC gene. Curr. Microbiol. 50, 38-42. (Pubitemid 40655427)
19. Piknova L., Stefanovicova A., Drahovska H., Sasik M., Kuchta, T. (2002). Detection of Salmonella in food, equivalent to ISO 6579, by a three-days polimerase chain reaction-based method. Food Control. 13, 191-194. (Pubitemid 34135389)
20. Rodriguez-Lazaro D., Hernandez M., Esteve T., Hoorfar J., Pla M. (2003). A rapid and direct re-al time PCR-based method for identification of Salmonella spp.J. Microbiol. Methods. 54, 381-390. (Pubitemid 36773981)
21. Yang C., Jiang Y., Huang K., Zhu C., Yin Y., Gong J.H., Yu H. (2004). A real-time PCR assay for the detection and quantitation of Campylobacter jejuni using SYBR Green I and the Light Cycler. Yale J. Biol. Med. 77, 125-132. (Pubitemid 44387538)