Two methods for construction of internal amplification controls for the detection of Escherichia coli O157 by polymerase chain reaction

Molecular and Cellular Probes

Volumn 16, Issue 5, 2002, Pages 335-339

  Abdulmawjood, Amir ^a   Roth, S ^a   Bulte M ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Escherichia coli O157; False negative results; Internal amplification control; PCR

Indexed keywords

BACTERIAL DNA; PRIMER DNA;

ACCURACY; AMPLICON; ARTICLE; BACTERIUM DETECTION; CONTROL; DNA SEQUENCE; DNA SYNTHESIS; DNA TEMPLATE; ESCHERICHIA COLI; FOOD CONTAMINATION; FOOD CONTROL; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ESCHERICHIA COLI O157; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; STANDARD;

ESCHERICHIA COLI;

DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI O157; FOOD CONTAMINATION; FOOD MICROBIOLOGY; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS;

EID: 0036812484     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.2002.0431     Document Type: Article

References (8)

1. Al-Soud, A. W. & Radström, P. (2000). Effects of amplification facilitators on diagnostic PCR in the presence of blood, faeces and meat. Journal of Clinical Microbiology 38, 4463-70.
2. Ursi, D., Leven, M., Van Bever, H. P. & Goossens, H. (1998). Construction of an internal control for the detection of Chlamydia pneumoniae by PCR. Molecular and Cellular Probes 12, 235-38.
3. Rossen, L., Norskov, P., Holmstrom, K. & Rasmussen, O. F. (1992). Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. International Journal of Food Microbiology 17, 37-45.
4. Mengue Metogho, R., Deslandes, S. & Frost, E. H. (1999). Comparison of PCR protocols including positive controls for detection of Chlamydia pneumoniae in respiratory specimens. Molecular and Cellular Probes 13, 71-5.
5. Jones, R. N., Neale, M. L., Beattie, B., Westmoreland, D. & Fox, J. D. (2000). Development and application of a PCR-based method including an internal amplification control for diagnosis of congenital cytomegalovirus infection. Journal of Clinical Microbiology 38, 1-6.
6. Sachadyn, P. & Kur, J. (1998). The construction and use of a PCR internal amplification control. Molecular and Cellular Probes 12, 259-62.
7. Abdulmawjood, A., Roth, S. & Bülte, M. (2001). Evaluation of a PCR system for the detection of E. coli O157 based on rfbE O157 gene, The 42nd Congress on Food Hygiene; German Veterinary Society (DVG), Sept. 25-28 in Garmisch-Partenkirchen, Germany, pp. 368-373.
8. Brightwell, G., Pearce, M. & Leslie, D. (1998). Development of internal controls for PCR detection of Bacillus anthracis. Molecular and Cellular Probes 12, 367-77.