Influence of dichloroacetate (DCA) on lactate production and oxygen consumption in neuroblastoma cells: Is DCA a suitable drug for neuroblastoma therapy?

Cellular Physiology and Biochemistry

Volumn 29, Issue 3-4, 2012, Pages 373-380

  Niewisch, Marena Rebekka ^a   Kuçi, Zyrafete ^a   Wolburg, Hartwig ^a   Sautter, Mirjam ^a   Krampen, Lea ^a   Deubzer, Beate ^a   Handgretinger, Rupert ^a   Bruchelt, Gernot ^b  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Dichloroacetate; Lactate; Neuroblastoma; Oxygen consumption; Warburg effect

Indexed keywords

DICHLOROACETIC ACID; GLUCOSE; LACTIC ACID; PYRUVATE DEHYDROGENASE; REACTIVE OXYGEN METABOLITE;

APOPTOSIS; ARTICLE; CELL PROLIFERATION; GLUCOSE INTAKE; HUMAN; HUMAN CELL; IN VITRO STUDY; NEUROBLASTOMA CELL; OXYGEN CONSUMPTION; PRIORITY JOURNAL; RESPIRATORY CHAIN;

EID: 84859899701     PISSN: 10158987     EISSN: 14219778     Source Type: Journal    
DOI: 10.1159/000338492     Document Type: Article

References (31)

1. Warburg O: On the origin of cancer cells. Science 1956;123:309-314.
2. Semenza GL, Roth PH, Fang HM, Wang GL: Transcriptional regulation of genes encoding glycolytic enzymes by hypoxiainducible factor 1. J Biol Chem 1994;269:23757-23763. (Pubitemid 24293585)
3. Kim JW, Dang CV: Cancer's molecular sweet tooth and the Warburg effect. Cancer Res 2006;66:8927-8930. (Pubitemid 44521105)
4. Cairns RA, Harris IS, Mak TW: Regulation of cancer cell metabolism. Nature Rev Cancer 2011;11:85-95.
5. Levine AJ, Puzio-Kuter AM: The control of the metabolic switch in cancers by oncogenes and tumor suppressor genes. Science 2010;330:1340-1344.
6. Bonnet S, Archer SL, Allalunis-Turner J, Haromy A, Beaulieu C, Thompson R, Lee CT, Lopaschuk GD, Puttagunta L, Bonnet S, Harry G, Hashimoto K, Porter CJ, Andrade MA, Thebaud B, Michelakis ED: A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 2007;11:37-51. (Pubitemid 46054520)
7. Vander Heiden MG, Cantley LC, Thompson CB: Understanding the Warburg Effect: The metabolic requirements of cell proliferation. Sience 2009;324:1029-1033.
8. Gatenby RA, Gillies RJ: Why do cancers have high aerobic glycolysis? Nature Rev Cancer 2004;4:891-899. (Pubitemid 39472955)
9. Stacpoole PW, Kerr DS, Barnes C, Bunch ST, Carney PR, Fennell EM, Felitsyn NM, Gilmore RL, Greer M, Henderson GN, Hutson AD, Neiberger RE, O'Brien RG, Perkins LA, Quisling RG, Shroads AL, Shuster JJ, Silverstein JH, Theriaque DW, Valenstein E: Controlled clinical trial of dichloroacetate for treatment of congential lactic acidosis in children. Pediatrics 2006;117:1519-1531. (Pubitemid 46071378)
10. Michelakis ED, Webster L, Mackey JR: Dichloroacetate (DCA) as a potential metabolic-targeting therapy for cancer. Br J Cancer 2008;99:989-994.
11. Wong JYY, Huggins GS, Debidda M, Munchi NC, De Vivo I: Dichloroacetate induces apoptosis in endometrial cancer cells. Gynecol Oncol 2008;109:394-402.
12. Cao W, Yacoub S, Shiverick KT, Namiki K, Sakai Y, Porvasnik S, Urbanek S, Rosser CJ: Dichloroacetate (DCA) sensitizes both wild-type and over expressing Bcl-2 prostate cancer cells in vitro to radiation. Prostate 2008;68:1223-1231.
13. Sun W, Zhou S, Chang SS, McFate T, Verma A, Califano JA: Mitochondrial mutations contribute to HIF-1á accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma. Clin Cancer Res 2009;15:476-484.
14. Heshe D, Hoogestraat S, Brauckmann C, Karst U, Boos J, Lanvers-Kaminsky C: Dichloroacetate metabolically targeted therapy defeats cytotoxicity of standard anticancer drugs. Cancer Chemother Pharmacol 2011;67:647-655.
15. Madhok BM, Yeluri S, Perry SL, Hughes TA, Jayne DG: Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells. Br J Cancer 2010;102:1746-52.
16. Papandreou I, Goliasova T, Denko NC: Anticancer drugs that target metabolism: is dichloroacetate the new paradigm? Int J Cancer 2011;128:1001-1008.
17. Michelakis ED, Sutendra G, Dromparis P, Webster L, Haromy A, Niven E, Maguire C, Gammer TL, Mackey JR, Fulton D, Abdulkarim B, McMurtry MS, Petruk KC: Metabolic modulation of gliobastoma with dichloroacetate. Sci Transl Med 2010;2:31ra34.
18. Flavin DF: Non-Hodgkin's Lymphoma reversal with dichloroacetate. J Oncol 2010; Article ID414726.
19. Tennant DA, Durán RV, Gottlieb E: Targeting metabolic transformation for cancer therapy. Nature Rev Cancer 2010;10:267-277.
20. Rudolph G, Schilbach-Stückle K, Handgretinger R, Kaiser P, Hameister H: Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS. Human Genet 1991;86:562-566.
21. Lowry OH, Rosebrough NJ, Lewis Farr A, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275.
22. Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63. (Pubitemid 14203433)
23. Koppenol WH, Bounds PL, Dang CV: Otto Warburg's contributions to current concepts of cancer metabolism. Nature Rev Cancer 2011;11:325-337.
24. Thompson CB: Metabolic enzymes as oncogenes or tumor suppressors. N Engl J Med 2009;360:813-815.
25. Reitman ZJ, Hai Yan: Isocitrate dehydrogenase 1 and 2 mutations in cancer: Alterations at a crossroads of cellular metabolism. J Natl Cancer Inst 2010;102:932-941.
26. Matoba S, Kang JG, Patino WD, WraggA, Boehm M, Gavrilova O, Hurley PJ, Bunz F, Hwang PM: p53 regulates mitochondrial respiration. Science 2006;312:1650-1653. (Pubitemid 43902663)
27. Eggert A, Schulte JH: A small kiss of death for cancer. Nature med 2010;16:1079-1081.
28. Swarbrick A, Woods SL, Shaw A, Balakrishnan A, Phua Y, Nguyen A, Chanthery Y, Lim L, Ashton LJ, Judson RL, Huskey N, Blelloch R, Haber M, Norris MD, Lengyel P, Hackett CS, Preiss T, Chetcuti A, Sullivan CS, Marcusson EG, Weiss W, L'Etoile N, Goga A: miR- 380-5p represses p53 to control cellular survival and is associated with poor outcome in MYCN-amplified neuroblastoma. Nat Med 2010;16:1134-1140.
29. Feichtinger RG, Zimmermann F, Mayr JA, Neureiter D, Hauser-Kronberger C, Schilling FH, Jones N, Sperl W, Kofler B: Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma. BMC Cancer 2010;10:149
30. Vella S, Conti M, Tasso R, Cancedda R, Pagano A: Dichloroacetate inhibits neuroblastoma growth by specifically acting against malignant undifferentiated cells. Int J Cancer 2012;130:1484-1493.
31. Deubzer B, Mayer F, Kuçi Z, Niewisch M, Merkel G, Handgretinger R, Bruchelt G: H2O2-mediated cytotoxicity of pharmacologic ascorbate concentrations to neuroblastoma cells: Potential role of lactate and ferritin. Cell Physiol Biochem 2010;25:767-774.