FRET microscopy in the living cell: Different approaches, strengths and weaknesses

BioEssays

Volumn 34, Issue 5, 2012, Pages 369-376

  Padilla Parra, Sergi ^a   Tramier, Marc ^b,c  

^a EMORY UNIVERSITY   (United States)

^b UNIVERSITÉ DE RENNES 1   (France)

^c UNIVERSITÉ DE RENNES 1   (France)

Author keywords

Biochemical activities; FLIM; Fluorescence microscopy; Protein protein interactions

Indexed keywords

GUANOSINE TRIPHOSPHATASE; VASCULOTROPIN;

ARTICLE; BLEACHING; CALCIUM TRANSPORT; DATA ANALYSIS; ENERGY TRANSFER; ENZYME ACTIVATION; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE MICROSCOPY; FORSTER RESONANCE ENERGY TRANSFER MICROSCOPY; MOLECULAR INTERACTION; PHOTODYNAMICS; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; QUANTUM YIELD; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION; STEADY STATE;

FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; PROTEIN INTERACTION MAPPING;

EID: 84859848908     PISSN: 02659247     EISSN: 15211878     Source Type: Journal    
DOI: 10.1002/bies.201100086     Document Type: Article

References (53)

1. Förster T. 1948. Intermolecular energy migration and fluorescence. Ann Phys 6: 55- 75.
2. Weiss S. 1999. Fluorescence spectroscopy of single biomolecules. Science 283: 1676- 83.
3. Tsien R. 1998. The green fluorescent protein. Ann Rev Biochem 67: 509- 44.
4. Zacharias DA, Baird GS, Tsien RY. 2000. Recent advances in technology for measuring and manipulating cell signals. Curr Opin Neurobiol 10: 416- 21.
5. Conzelmann H, Saez-Rodriguez J, Sauter T, Bullinger E, et al. 2004. Reduction of mathematical models of signal transduction networks: simulation-based approach applied to EGF receptor signalling. Syst Biol 1: 159- 69.
6. Gordon GW, Berry G, Liang XH, Levine B, et al. 1998. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys J 74: 2702- 13.
7. Xia Z, Liu Y. 2001. Reliable and global measurement of fluorescence resonance energy transfer. Biophys J 81: 2395- 402.
8. Elangovan M, Wallrabe H, Chen Y, Day RM, et al. 2003. Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy. Methods 29: 58- 73.
9. Sun YS, Perisamy A. 2010. Additional correction for energy transfer efficiency calculation in filter-based Forster resonance energy transfer microscopy for more accurate results. J Biomed Opt 15: 020513.
10. Lundin K, Blomberg K, Nordstrom T, Lindqvist C. 2001. Development of a time-resolved fluorescence resonance energy transfer assay (cell-TR-FRET) for protein detection on intact cells. Anal Biochem 299: 92- 7.
11. Hoppe A, Christensen K, Swanson JA. 2002. Fluorescence resonance energy transfer-based stoichiometry in living cells. Biophys J 83: 3652- 64.
12. Zal T, Gascoigne RJ. 2004. Photobleaching-corrected FRET efficiency imaging of live cells. Biophys J 86: 3923- 39.
13. Zimmermann T, Rietdorf J, Girod A, Georget V, et al. 2002. Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair. FEBS Lett 531: 245- 9.
14. Chen H, Puhl HL, Koushik SV, Vogel SS, et al. 2006. Measurement of FRET efficiency and ratio of donor to acceptor concentration in living cells. Biophys J 91: L39- 41.
15. Zimmermann T, Siegert F. 1998. 4D confocal microscopy of Dictyostelium discoideum morphogenesis and its presentation on the internet. Dev Genes Evol 208: 411- 20.
16. Neher R, Neher E. 2004. Applying spectral fingerprinting to the analysis of FRET imaging. Microsc Res Tech 64: 185- 95.
17. Aoki M, Matsuda M. 2009. Visualization of small GTPase activity with fluorescence resonance energy transfer based biosensors. Nat Protoc 4: 1623- 31.
18. Nakamura T, Kurokawa K, Kiyokawa E, Matsuda M. 2006. Analysis of the spatiotemporal activation of Rho GTPases using Raichu probes. Methods Enzymol 406: 315- 22.
19. Higashi M, Yu J, Tsuchiya H, Saito T, et al. 2010. Visualization of the activity of Rac1 small GTPase in a cell. Acta Histochem Cytochem 29: 163- 8.
20. Wallrabe H, Periasamy A. 2005. Imaging protein molecules using FRET and FLIM microscopy. Curr Opin Biotechnol 16: 19- 27.
21. Tramier M, Gautier I, Piolot T, Ravalet S, et al. 2002. Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells. Biophys J 83: 3570- 7.
22. Storez H, Scott MGH, Issafras H, Burtey A, et al. 2005. Homo and hetero-oligomerization of B-arrestins in living cells. J Biol Chem 280: 40210- 5.
23. Delbarre E, Tramier M, Coppey-Moisan M, Gaillard C, et al. 2006. The truncated prelamin A in Hutchinson-Gilford progeria syndrome alters segregation of A-type and B-type lamin. Hum Mol Gen 15: 1113- 22.
24. Padilla-Parra S, Audugé N, Lalucque H, Mevel JC, et al. 2009. Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition. Biophys J 97: 2368- 76.
25. Boularan C, Scott MGH, Bourougaa K, Bellal M, et al. 2007. B-Arrestin 2 oligomerization controls Mdm2-dependent inhibition of p53. Proc Natl Acad Sci USA 104: 18061- 6.
26. Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M. 2008. Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells. Biophys J 95: 2976- 88.
27. Yamada H, Padilla-Parra S, Park SJ, Itoh T, et al. 2009. Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. J Biol Chem 284: 34244- 56.
28. Dowling K, Dayel M, Lever MJ, French PMW, et al. 1998. Fluorescence imaging with picosecond resolution for biomedical application. Opt Lett 23: 810- 2.
29. Grant DM, Zhang W, McGhee EJ, Bunney TD, et al. 2008. Multiplexed FRET to image multiple signaling events in live cells. Biophys J 95: L69- 71.
30. Tramier M, Zahid M, Mevel JC, Masse MJ, et al. 2006. Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells. Microsc Res Tech 11: 933- 42.
31. Digman MA, Caiolfa VR, Zamai M, Gratton E. 2008. The phasor approach of fluorescence lifetime imaging analysis. Biophys J 94: L14- 6.
32. Caiolfa VR, Zamai M, Malengo G, Andolfo A, et al. 2007. Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies. J Cell Biol 179: 1067- 82.
33. Leray A, Spriet C, Trinel D, Blossey R, et al. 2011. Quantitative comparison of polar approach versus fitting method in time domain FLIM image analysis. Cytometry A 79: 149- 58.
34. Grecco HE, Roda-Navarro P, Verveer PJ. 2009. Global analysis of time correlated single photon counting FRET-FLIM data. Opt Expr 17: 6493- 508.
35. Grecco HE, Roda-Navarro P, Girod A, Hou J, et al. 2010. In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays. Nat Methods 7: 467- 72.
36. Lakowicz JR. 2006. Principles of Fluorescence Spectroscopy, 3rd edition. New York: Springer.
37. Peter M, Ameer-Beg SM, Hughes M, Keppler M, et al. 2005. Multiphoton FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions. Biophys J 88: 1224- 37.
38. Yudushkin I, Vale RD. 2010. Imaging T-cell receptor activation reveals accumulation of tyrosine-phosphorylated CD3ζ in the endosomal compartment. Proc Natl Acad Sci USA 107: 22128- 33.
39. Zhuang X, Bartley LE, Babcock HP, Russell R, et al. 2000. A single molecule study of RNA catalysis and folding. Science 288: 2048- 51.
40. Roy R, Hohng S, Ha T. 2008. A practical guide to single molecule FRET. Nat Methods 5: 507- 16.
41. Murakoshi H, Lino R, Kobayashi T, Fujiwara T, et al. 2004. Single-molecule imaging analysis of Ras activation in living cells. Proc Natl Acad Sci USA 19: 7317- 22.
42. Sako Y, Minoghchi S, Yanagida T. 2000. Single-molecule imaging of EGFR signaling on the surface of living cells. Nat Cell Biol 2: 168- 72.
43. Ji W, Xu P, Li Z, Lu J, et al. 2008. Functional stoichiometry of the unitary calcium-release-activated calcium channel. Proc Natl Acad Sci USA 105: 13668- 73.
44. Webb S, Roberts SK, Needham SR, Tynan CJ, et al. 2008. Single-molecule imaging and fluorescence lifetime imaging microscopy show different structures for high and low affinity epidermal growth factor receptors in A431 cells. Biophys J 94: 803- 19.
45. Pantoja R, Rodriguez EA, Dibas M, Dougherty DA, et al. 2009. Single-molecule imaging of a fluorescent unnatural amino acid incorporated into nicotinic receptors. Biophys J 96: 226- 37.
46. Ulbrich MH, Isacoff E. 2007. Subunit counting in membrane-bound proteins. Nat Methods 4: 319- 21.
47. Karasawa S, Araki T, Nagai T, Mizuno H, et al. 2004. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J 381: 307- 12.
48. Kuner T, Augustine GJ. 2000. A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Neuron 27: 447- 59.
49. 2+ based on green fluorescent proteins and calmodulin. Nature 388: 882- 7.
50. 2+ imaging by a new calmodulin fusion molecule. Nat Struct Biol 8: 1069- 73.
51. Michizuki N, Yamashita S, Kurokawa K, Ohba Y, et al. 2000. Spatio-temporal images of growth-factor induced activation of Ras and Rap1. Nature 401: 1065- 8.
52. Yamada S, Nelson JW. 2007. Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion. J Biol Chem 178: 517- 27.
53. Padilla-Parra S, Auduge N, Coppey-Moisan M, Tramier M. 2011. Non-fitting based FRET FLIM analysis approaches applied to quantify protein-protein interactions in live cells. Biophys Rev 3: 63- 70.