Recent advances in technology for measuring and manipulating cell signals

Current Opinion in Neurobiology

Volumn 10, Issue 3, 2000, Pages 416-421

  Zacharias, David A ^a,b   Baird, Geoffrey S ^a   Tsien, Roger Y ^a,b  

^a San Diego   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

BIOLUMINESCENCE; ENERGY TRANSFER; FLUORESCENCE; PRIORITY JOURNAL; PROTEIN TRANSPORT; REVIEW; SIGNAL TRANSDUCTION;

EID: 0034083428     PISSN: 09594388     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-4388(00)00101-X     Document Type: Review

References (32)

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18. 4-CaM was determined in cells using a FRET assay. Being able to determine this relationship is important for understanding the cellular conditions in which calmodulin-dependent enzymes are either activated or inactivated.
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28. + channel during depolarization. The results are consistent with a rotation of the helices, providing a smaller motion than the large translation normal to the membrane postulated in previous models.
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32. Furuta T., Wang S.S-H., Dantzker J.L., Dore T.M., Bybee W.J., Callaway E.M., Denk W., Tsien R.Y. Brominated 7-hydroxycoumarin-4-ylmethyls: novel photolabile protecting groups with biologically useful cross-sections for two photon photolysis. Proc Natl Acad Sci USA. 96:1999;1193-1200. Bhc is the first caging group with the following properties: a high sensitivity to both one- and two-photon photolysis; solubility in aqueous solutions; and the ability to cage several classes of biologically important chemical including neurotransmitters and many second messengers. Two-photon photolysis has enabled mapping of glutamate sensitivity with optical sectioning resolution over the surface of neurons in brain slices.