Quantitative comparison of polar approach versus fitting method in time domain FLIM image analysis

Cytometry Part A

Volumn 79 A, Issue 2, 2011, Pages 149-158

  Leray, A ^a   Spriet, C ^a   Trinel, D ^a   Blossey, R ^a   Usson, Y ^b   Heliot L ^a  

^a UNIV LILLE   (France)

^b LABORATOIRE TIMC IMAG   (France)

Author keywords

F value; Fluorescence lifetime imaging microscopy (FLIM); Living cell; LSM; Molecular interactions; Phasor

Indexed keywords

ARTICLE; CELL LINE; CELL STRAIN HEK293; COMPARATIVE STUDY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; METHODOLOGY; MONTE CARLO METHOD; PHOTON; REGRESSION ANALYSIS;

CELL LINE, TRANSFORMED; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEK293 CELLS; HUMANS; LEAST-SQUARES ANALYSIS; MICROSCOPY, FLUORESCENCE; MONTE CARLO METHOD; PHOTONS;

EID: 79251473079     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20996     Document Type: Article

References (23)

1. Lakowicsz JR. Principles of Fluorescence Spectroscopy. New York: Plenum Publishers; 1999.
2. Selvin PR. The renaissance of fluorescence resonance energy transfer. Nat Struct Biol 2000; 7: 730-734.
3. Jares-Erijman EA, Jovin TM. FRET imaging. Nat Biotechnol 2003; 21: 1387-1395.
4. Gadella TWJ, Jovin TM, Clegg RM. Fluorescence lifetime imaging microscopy (FLIM)-Spatial-resolution of microstructures on the nanosecond time-scale. Biophys Chem 1993; 48: 221-239.
5. Booth MJ, Wilson T. Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. J Microsc (Oxford) 2004; 214: 36-42.
6. Leray A, Riquet FB, Richard E, Spriet C, Trinel D, Heliot L. Optimized protocol of a frequency domain fluorescence lifetime imaging microscope for FRET measurements. Microsc Res Tech 2009; 72: 371-379.
7. Becker W, Bergmann A, Hink MA, Konig K, Benndorf K, Biskup C. Fluorescence lifetime imaging by time-correlated single-photon counting. Microsc Res Tech 2004; 63: 58-66.
8. Suhling K, French PM, Phillips D. Time-resolved fluorescence microscopy. Photochem Photobiol Sci 2005; 4: 13-22.
9. Waharte F, Spriet C, Heliot L. Setup and characterization of a multiphoton FLIM instrument for protein-protein interaction measurements in living cells. Cytometry A 2006; 69A: 299-306.
10. Spriet C, Trinel D, Riquet F, Vandenbunder B, Usson Y, Heliot L. Enhanced FRET contrast in lifetime imaging. Cytometry A 2008; 73A: 745-753.
11. Padilla-Parra S, Auduge N, Coppey-Moisan M, Tramier M. Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells. Biophys J 2008; 95: 2976-2988.
12. Digman MA, Caiolfa VR, Zamai M, Gratton E. The phasor approach to fluorescence lifetime imaging analysis. Biophys J 2008; 94: L14-L16.
13. Jameson DM, Gratton E, Hall RD. The measurement and analysis of heterogeneous emissions by multifrequency phase and modulation fluorometry. Appl Spectrosc Rev 1984; 20: 55-106.
14. Clayton AH, Hanley QS, Verveer PJ. Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data. J Microsc 2004; 213 (Part 1): 1-5.
15. Redford GI, Clegg RM. Polar plot representation for frequency-domain analysis of fluorescence lifetimes. J Fluoresc 2005; 15: 805-815.
16. Gerritsen HC, Asselbergs MA, Agronskaia AV, Van Sark WG. Fluorescence lifetime imaging in scanning microscopes: Acquisition speed, photon economy and lifetime resolution. J Microsc 2002; 206 (Part 3): 218-224.
17. Li DU, Bonnist E, Renshaw D, Henderson R. On-chip, time-correlated, fluorescence lifetime extraction algorithms and error analysis. J Opt Soc Am A Opt Image Sci Vis 2008; 25: 1190-1198.
18. Hall P, Sellinger B. Better estimates of exponential decay parameters. J Phys Chem 1981; 85: 2941-2946.
19. Köllner M, Wolfrum J. How many photons are necessary for fluorescence-lifetime measurements? Chem Phys Lett 1992; 200: 199-204.
20. Leray A, Spriet C, Trinel D, Heliot L. Three-dimensional polar representation for multispectral fluorescence lifetime imaging microscopy. Cytometry A 2009; 75A: 1007-1014.
21. Philip J, Carlsson K. Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging. J Opt Soc Am A 2003; 20: 368-379.
22. Zhong H, Wu X, Huang H, Fan Q, Zhu Z, Lin S. Vertebrate MAX-1 is required for vascular patterning in zebrafish. Proc Natl Acad Sci USA 2006; 103: 16800-16805.
23. Barber PR, Ameer-Beg SM, Gilbey J, Carlin LM, Keppler M, Ng TC, Vojnovic B. Multiphoton time-domain fluorescence lifetime imaging microscopy: Practical application to protein-protein interactions using global analysis. J R Soc Interface 2009; 6: S93-S105.