Development of a time-resolved fluorescence resonance energy transfer assay (Cell TR-FRET) for protein detection on intact cells

Analytical Biochemistry

Volumn 299, Issue 1, 2001, Pages 92-97

  Lundin, Karolina ^a,b   Blomberg, Kaj ^a,b   Nordström, Tommy ^a,b   Lindqvist, Christer ^a,b  

^a ÅBO AKADEMI UNIVERSITY   (Finland)

^b Wallac Oy   (Finland)

Author keywords

Biotin; Cy5; Europium; FRET; IL 2R

Indexed keywords

ANTIBODIES; BINDING ENERGY; CELL MEMBRANES; COENZYMES; ENERGY TRANSFER; EUROPIUM; EXCITED STATES; FORSTER RESONANCE ENERGY TRANSFER; SCREENING;

BIOTIN; FRET; HOMOGENEOUS ASSAYS; MEMBRANE-ASSOCIATED PROTEINS; RECEPTOR PROTEINS; RECOMBINANT BACULOVIRUS; SPECIFIC COMPONENT; TIME-RESOLVED FLUORESCENCE;

CYTOLOGY;

BIOTIN; EUROPIUM; FLUOROCHROME; INTERLEUKIN 2 RECEPTOR; INTERLEUKIN 2 RECEPTOR ANTIBODY; STREPTAVIDIN;

ANTIBODY SPECIFICITY; ARTICLE; ASSAY; BACULOVIRUS; CELL LABELING; CELL MEMBRANE; CHELATION; CONTROLLED STUDY; DRUG SCREENING; ENERGY TRANSFER; FLUORESCENCE; HUMAN; METHODOLOGY; PRIORITY JOURNAL; PROTEIN DETERMINATION; TIME; VIRUS RECOMBINANT;

UNIDENTIFIED BACULOVIRUS;

EID: 0035577918     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5370     Document Type: Article

References (27)

1. Zwischen molekulare energiewanderung und fluoreszenz
2. Fluorescent excitation transfer immunoassay: A general method for determination of antigens
3. Terbium and rhodamine as labels in a homogeneous time-resolved flourometric energy transfer assay of the β subunit of human chorionic gonadotropin in serum
4. Energy-transfer immunoassay: A study of the experimental parameters in an assay for human serum albumin
5. Time-resolved detection of energy transfer: Theory and application to immunoassays
6. High throughput screening for human interferon-γ production inhibitor using homogeneous time-resolved fluorescence
7. Fluorescence resonance energy transfer measurements on cell surfaces: A spectroscopic tool for determining protein interactions
8. Preassembly of interleukin-2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7 and IL-15: A fluorescence resonance energy transfer study
9. Flow cytometric resonance energy transfer measurements support the association of a 95-kDa peptide termed T27 with the 55-kDa Tac peptide
10. Fluorescence resonance energy transfer: FRET studies of ligand binding to cell surface receptors
11. Fluoroimmunoassay: Present status and key problems
12. Europium as a label in time-resolved immunofluorometric assays
13. Immunoassays with time-resolved fluorescence spectroscopy: Principles and applications
14. Luminescence resonance energy transfer
15. The use of the avidin-biotin complex as a tool in molecular biology
16. Identification of surface proteins on bovine leukocytes by a biotin-avidin protein blotting technique
17. Biotinylation: An alternative to radioiodination for the identification of cell surface antigens in immunoprecipitates
18. Selective in vitro growth of lymphocytes from normal human bone marrows
19. Interleukin-2: Inception, impact, and implications
20. Low and high affinity cellular receptors for interleukin-2: Implications for the level of Tac antigen
21. An associated molecule, p64, with IL-2 receptor β-chain: Its possible involvement in the formation of the functional intermediate-affinity IL-2 receptor complex
22. Cloning of the γ chain of the human IL-2 receptor
23. The IL-2/IL-2 receptor system: A current overview
24. A monoclonal antibody 7G7/B6, binds to an epitope on the human interleukin-2 (IL-2) receptor that is distinct from that recognized by IL-2 or anti-Tac
25. Expression of human IL-2 receptor α- and β-chains using the baculovirus expression system
26. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera: Nocuidae)
27. Homogeneous time resolved IL-2- IL-2Rα assay using fluorescence resonance energy transfer