Heterologous expression and purification of Arabidopsis thaliana VIM1 protein: In vitro evidence for its inability to recognize hydroxymethylcytosine, a rare base in Arabidopsis DNA

Protein Expression and Purification

Volumn 83, Issue 1, 2012, Pages 104-111

  Yao, Qin ^a   Song, Chun Xiao ^b   He, Chuan ^b   Kumaran, Desigan ^a   Dunn, John J ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

5 Methyl cytosine 5 hydroxymethyl cytosine; Arabidopsis thaliana; Electrophoretic mobility shift (EMSA) assay; Fluorescence anisotrophy (FA) assays; VIM1 (Variant in Methylation 1) protein

Indexed keywords

5 HYDROXYMETHYLCYTOSINE; 5 METHYLCYTOSINE; 5-HYDROXYMETHYLCYTOSINE; ARABIDOPSIS PROTEIN; CYTOSINE; DNA BINDING PROTEIN; DRUG DERIVATIVE; PLANT DNA; RECOMBINANT PROTEIN; VIM1 PROTEIN, ARABIDOPSIS;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; DNA METHYLATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; SPECTROFLUOROMETRY;

5-METHYLCYTOSINE; ARABIDOPSIS PROTEINS; CYTOSINE; DNA METHYLATION; DNA, PLANT; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ESCHERICHIA COLI; MODELS, MOLECULAR; PROTEIN BINDING; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

ARABIDOPSIS; ARABIDOPSIS THALIANA; ESCHERICHIA COLI; MAMMALIA;

EID: 84859387765     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.03.003     Document Type: Article

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