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Volumn 62, Issue 1, 2008, Pages 98-103

Cloning, purification and initial characterization of E. coli McrA, a putative 5-methylcytosine-specific nuclease

Author keywords

C5 methylcytosine (m5C); CpG island affinity purification; DNA binding; DNA methylation; McrA; Zn2+ finger

Indexed keywords

5 METHYLCYTOSINE; BACTERIAL DNA; ENDODEOXYRIBONUCLEASE MCRA; RECOMBINANT PROTEIN; RESTRICTION ENDONUCLEASE;

EID: 52949085449     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.06.016     Document Type: Article
Times cited : (20)

References (28)
  • 1
    • 0028340280 scopus 로고
    • Purification of CpG islands using a methylated DNA binding column
    • Cross S.H., Charlton J.A., Nan X., and Bird A.P. Purification of CpG islands using a methylated DNA binding column. Nat. Genet. 6 (1994) 236-244
    • (1994) Nat. Genet. , vol.6 , pp. 236-244
    • Cross, S.H.1    Charlton, J.A.2    Nan, X.3    Bird, A.P.4
  • 3
    • 33745700202 scopus 로고    scopus 로고
    • Genome-wide profiling of CpG methylation identifies novel targets of aberrant hypermethylation in myeloid leukemia
    • Gebhard C., Schwarzfischer L., Pham T.H., Schilling E., Klug M., Andreesen R., and Rehli M. Genome-wide profiling of CpG methylation identifies novel targets of aberrant hypermethylation in myeloid leukemia. Cancer Res. 66 (2006) 6118-6128
    • (2006) Cancer Res. , vol.66 , pp. 6118-6128
    • Gebhard, C.1    Schwarzfischer, L.2    Pham, T.H.3    Schilling, E.4    Klug, M.5    Andreesen, R.6    Rehli, M.7
  • 5
    • 33748055105 scopus 로고    scopus 로고
    • MIRA-assisted microarray analysis, a new technology for the determination of DNA methylation patterns, identifies frequent methylation of homeodomain-containing genes in lung cancer cells
    • Rauch T., Li H., Wu X., and Pfeifer G.P. MIRA-assisted microarray analysis, a new technology for the determination of DNA methylation patterns, identifies frequent methylation of homeodomain-containing genes in lung cancer cells. Cancer Res. 66 (2006) 7939-7947
    • (2006) Cancer Res. , vol.66 , pp. 7939-7947
    • Rauch, T.1    Li, H.2    Wu, X.3    Pfeifer, G.P.4
  • 6
    • 27744443057 scopus 로고    scopus 로고
    • Methylated-CpG island recovery assay: a new technique for the rapid detection of methylated-CpG islands in cancer
    • Rauch T., and Pfeifer G.P. Methylated-CpG island recovery assay: a new technique for the rapid detection of methylated-CpG islands in cancer. Lab. Invest. 85 (2005) 1172-1180
    • (2005) Lab. Invest. , vol.85 , pp. 1172-1180
    • Rauch, T.1    Pfeifer, G.P.2
  • 7
    • 33845880624 scopus 로고    scopus 로고
    • Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription
    • Zilberman D., Gehring M., Tran R.K., Ballinger T., and Henikoff S. Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription. Nat. Genet. 39 (2007) 61-69
    • (2007) Nat. Genet. , vol.39 , pp. 61-69
    • Zilberman, D.1    Gehring, M.2    Tran, R.K.3    Ballinger, T.4    Henikoff, S.5
  • 8
    • 39849085506 scopus 로고    scopus 로고
    • Substrate specificity of new methyl-directed DNA endonuclease GlaI
    • Tarasova G.V., Nayakshina T.N., and Degtyarev S.K. Substrate specificity of new methyl-directed DNA endonuclease GlaI. BMC Mol. Biol. 9 (2008) 7
    • (2008) BMC Mol. Biol. , vol.9 , pp. 7
    • Tarasova, G.V.1    Nayakshina, T.N.2    Degtyarev, S.K.3
  • 9
    • 0023274383 scopus 로고
    • Site-specific methylases induce the SOS DNA repair response in Escherichia coli
    • Heitman J., and Model P. Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J. Bacteriol. 169 (1987) 3243-3250
    • (1987) J. Bacteriol. , vol.169 , pp. 3243-3250
    • Heitman, J.1    Model, P.2
  • 11
    • 0022872027 scopus 로고
    • Escherichia coli K-12 restricts DNA containing 5-methylcytosine
    • Raleigh E.A., and Wilson G. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc. Natl. Acad. Sci. USA 83 (1986) 9070-9074
    • (1986) Proc. Natl. Acad. Sci. USA , vol.83 , pp. 9070-9074
    • Raleigh, E.A.1    Wilson, G.2
  • 12
    • 0017232789 scopus 로고
    • Modification and restriction of T-even bacteriophages. In vitro degradation of deoxyribonucleic acid containing 5-hydroxymethylctosine
    • Fleischman R.A., Cambell J.L., and Richardson C.C. Modification and restriction of T-even bacteriophages. In vitro degradation of deoxyribonucleic acid containing 5-hydroxymethylctosine. J. Biol. Chem. 251 (1976) 1561-1570
    • (1976) J. Biol. Chem. , vol.251 , pp. 1561-1570
    • Fleischman, R.A.1    Cambell, J.L.2    Richardson, C.C.3
  • 14
    • 0024362775 scopus 로고
    • Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12
    • Raleigh E.A., Trimarchi R., and Revel H. Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12. Genetics 122 (1989) 279-296
    • (1989) Genetics , vol.122 , pp. 279-296
    • Raleigh, E.A.1    Trimarchi, R.2    Revel, H.3
  • 15
    • 0031981857 scopus 로고    scopus 로고
    • Dependence of McrBC cleavage on distance between recognition elements
    • Stewart F.J., and Raleigh E.A. Dependence of McrBC cleavage on distance between recognition elements. Biol. Chem. 379 (1998) 611-616
    • (1998) Biol. Chem. , vol.379 , pp. 611-616
    • Stewart, F.J.1    Raleigh, E.A.2
  • 16
    • 0025837124 scopus 로고
    • Cloning and structural characterization of the mcrA locus of Escherichia coli
    • Hiom K., and Sedgwick S.G. Cloning and structural characterization of the mcrA locus of Escherichia coli. J. Bacteriol. 173 (1991) 7368-7373
    • (1991) J. Bacteriol. , vol.173 , pp. 7368-7373
    • Hiom, K.1    Sedgwick, S.G.2
  • 17
    • 0033822258 scopus 로고    scopus 로고
    • Atomic model of the 5-methylcytosine-specific restriction enzyme McrA reveals an atypical zinc finger and structural similarity to ββα-Me endonucleases
    • Bujnicki J.M., Radlinska M., and Rychlewski L. Atomic model of the 5-methylcytosine-specific restriction enzyme McrA reveals an atypical zinc finger and structural similarity to ββα-Me endonucleases. Mol. Microbiol. 37 (2000) 1280-1281
    • (2000) Mol. Microbiol. , vol.37 , pp. 1280-1281
    • Bujnicki, J.M.1    Radlinska, M.2    Rychlewski, L.3
  • 18
    • 51249170212 scopus 로고
    • Molecular cloning and sequencing of mcrA locus and identification of McrA protein in Escherichia coli
    • Ramalingam R., Prasad R., Shivapriya R., and Dharmalingam K. Molecular cloning and sequencing of mcrA locus and identification of McrA protein in Escherichia coli. J. Biosci. 17 (1992) 217-232
    • (1992) J. Biosci. , vol.17 , pp. 217-232
    • Ramalingam, R.1    Prasad, R.2    Shivapriya, R.3    Dharmalingam, K.4
  • 19
    • 0029031841 scopus 로고
    • Expression of the mcrA gene of Escherichia coli is regulated posttranscriptionally, possibly by sequestration of the Shine-Dalgarno region
    • Shivapriya R., Prasad R., Narayanan I.L., Krishnaswamy S., and Dharmalingam K. Expression of the mcrA gene of Escherichia coli is regulated posttranscriptionally, possibly by sequestration of the Shine-Dalgarno region. Gene 157 (1995) 201-207
    • (1995) Gene , vol.157 , pp. 201-207
    • Shivapriya, R.1    Prasad, R.2    Narayanan, I.L.3    Krishnaswamy, S.4    Dharmalingam, K.5
  • 20
    • 0020964297 scopus 로고
    • Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
    • Dunn J.J., and Studier F.W. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J. Mol. Biol. 166 (1983) 477-535
    • (1983) J. Mol. Biol. , vol.166 , pp. 477-535
    • Dunn, J.J.1    Studier, F.W.2
  • 21
    • 14844294424 scopus 로고    scopus 로고
    • Protein production by auto-induction in high density shaking cultures
    • Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41 (2005) 207-234
    • (2005) Protein Expr. Purif. , vol.41 , pp. 207-234
    • Studier, F.W.1
  • 22
    • 3242877618 scopus 로고    scopus 로고
    • DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data
    • Whitmore L., and Wallace B.A. DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acids Res. 32 (2004) W668-W673
    • (2004) Nucleic Acids Res. , vol.32
    • Whitmore, L.1    Wallace, B.A.2
  • 23
    • 45849096536 scopus 로고    scopus 로고
    • Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases
    • Whitmore L., and Wallace B.A. Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases. Biopolymers 89 (2008) 392-400
    • (2008) Biopolymers , vol.89 , pp. 392-400
    • Whitmore, L.1    Wallace, B.A.2
  • 24
    • 0027447099 scopus 로고
    • A self-consistent method for the analysis of protein secondary structure from circular dichroism
    • Sreerama N., and Woody R.W. A self-consistent method for the analysis of protein secondary structure from circular dichroism. Anal. Biochem. 209 (1993) 32-44
    • (1993) Anal. Biochem. , vol.209 , pp. 32-44
    • Sreerama, N.1    Woody, R.W.2
  • 25
    • 33746807366 scopus 로고    scopus 로고
    • Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation
    • Agius F., Kapoor A., and Zhu J.K. Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation. Proc. Natl. Acad. Sci. USA 103 (2006) 11796-11801
    • (2006) Proc. Natl. Acad. Sci. USA , vol.103 , pp. 11796-11801
    • Agius, F.1    Kapoor, A.2    Zhu, J.K.3
  • 26
    • 33845866061 scopus 로고    scopus 로고
    • The DNA glycosylase/lyase ROS1 functions in pruning DNA methylation patterns in Arabidopsis
    • Zhu J., Kapoor A., Sridhar V.V., Agius F., and Zhu J.K. The DNA glycosylase/lyase ROS1 functions in pruning DNA methylation patterns in Arabidopsis. Curr. Biol. 17 (2007) 54-59
    • (2007) Curr. Biol. , vol.17 , pp. 54-59
    • Zhu, J.1    Kapoor, A.2    Sridhar, V.V.3    Agius, F.4    Zhu, J.K.5
  • 28
    • 4344643339 scopus 로고    scopus 로고
    • Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease
    • Anton B.P., and Raleigh E.A. Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease. J. Bacteriol. 186 (2004) 5699-5707
    • (2004) J. Bacteriol. , vol.186 , pp. 5699-5707
    • Anton, B.P.1    Raleigh, E.A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.