Diversity of pharmacological properties in Chinese and European medicinal plants: Cytotoxicity, antiviral and antitrypanosomal screening of 82 herbal drugs

Diversity

Volumn 3, Issue 4, 2011, Pages 547-580

  Herrmann, Florian ^a   Romero, Marta R ^b   Blazquez, Alba G ^b   Kaufmann, Dorothea ^a   Ashour, Mohamed L ^c   Kahl, Stefan ^d   Marin, Jose J G ^b   Efferth, Thomas ^e   Wink, Michael ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY OF SALAMANCA   (Spain)

^c African Union Organization St Abbassia   (Egypt)

^d RHI AG   (Austria)

^e JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Anticancer drugs; BVDV; Cytotoxicity; Gastrointestinal tumors; Hepatitis B; Hepatitis C; HepG2 2.2.15; Traditional Chinese medicine; Trypanosoma brucei brucei

Indexed keywords

CANCER; DIGESTIVE SYSTEM DISORDER; FLAGELLATE; HEPATITIS; MEDICINAL PLANT; TOXICITY;

ALPINIA; ALPINIA GALANGA; ALPINIA OXYPHYLLA; ARCTIUM LAPPA; BOVINAE; BOVINE VIRAL DIARRHEA VIRUS 1; CELOSIA; CELOSIA CRISTATA; COPTIS CHINENSIS; EVODIA; EVODIA LEPTA; FLAVIVIRUS; GLYCYRRHIZA; HEDYOTIS; HEDYOTIS DIFFUSA; HEPATITIS B VIRUS; HEPATITIS C VIRUS; HOUTTUYNIA CORDATA; KADSURA; KADSURA LONGIPEDUNCULATA; OPHIOGLOSSUM VULGATUM; PANAX GINSENG; PANAX NOTOGINSENG; SAPOSHNIKOVIA DIVARICATA; SELAGINELLA; SELAGINELLA TAMARISCINA; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA BRUCEI BRUCEI;

EID: 84859306021     PISSN: None     EISSN: 14242818     Source Type: Journal    
DOI: 10.3390/d3040547     Document Type: Article

References (99)

1. Jiangsu New Medicine College Editorial Board. A Grand Dictionary of Chinese Medicinal Herbs, 3rd ed.; Shanghai Science Technology Publishing Co.: Shanghai, China, 1995; p. 2754.
2. Efferth, T.; Herrmann, F.; Tahrani, A.; Wink, M. Cytotoxic activity towards cancer cells of arteanuine B, artemisitene, scopoletin and 1,8-cineole derived from Artemisia annua L. in comparison to artemisinin. Phytomedicine 2011, 18, 959-969.
3. Efferth, T.; Romero, M.R.; Wolf, D.G.; Stamminger, T.; Marin, J.J.; Marschall, M. The antiviral activities of artemisinin and artesunate. Clin. Infect. Dis. 2008, 47, 804-811.
4. Efferth, T.; Li, P.C.; Konkimalla, V.S.; Kaina, B. From traditional Chinese medicine to rational cancer therapy. Trends Mol. Med. 2007, 13, 353-361.
5. Wohlfarth, C.; Efferth, T. Natural products as promising drug candidates for the treatment of hepatitis B and C. Acta Pharmacol. Sin. 2009, 30, 25-30.
6. Romero, M.R.; Serrano, M.A.; Vallejo, M.; Efferth, T.; Alvarez, M.; Marin, J.J. Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV). Planta Med. 2006, 72, 1169-1174.
7. Romero, M.R.; Efferth, T.; Serrano, M.A.; Castaño, B.; Macias, R.I.; Briz, O.; Marin, J.J. Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an "in vitro" replicative system. Antiviral Res. 2005, 68, 75-83.
8. Youns, M.; Hoheisel, J.D.; Efferth, T. Traditional Chinese medicines (TCMs) for molecular targeted therapies of tumours. Curr. Drug. Discov. Technol. 2010, 7, 37-45.
9. Van Wyk, B.E.; Wink, M. Medicinal Plants of the World, 1st ed.; BRIZA: Pretoria, South Africa, 2004.
10. Heinrich, M.; Leonti, M.; Nebel, S.; Peschel, W. "Local Food-Nutraceuticals": An example of a multidisciplinary research project on local knowledge. J. Physiol. Pharmacol. 2005, 56, 5-22.
11. Heinrich, M.; Lee Teoh, H. Galanthamine from snowdrop-The development of a modern drug against Alzheimer's disease from local Caucasian knowledge. J. Ethnopharmacol. 2004, 92, 147-162.
12. Wink, M.; Schimmer, O. Molecular modes of action of defensive secondary metabolites. In Annual Plant Reviews Volume 39: Functions and Biotechnology of Plant Secondary Metabolites, 2ne ed.; Wink, M., Ed.; Wiley-Blackwell: Oxford, UK, 2010; Chapter 2, pp. 21-161.
13. Dr. Duke's Phytochemical and Ethnobotanical Databases. Fulton, Maryland, USA, 2011. Available online: http://www.ars-grin.gov/duke/plants.html (accessed on 05 August 2011).
14. Tanikawa, K. Pathogenesis and treatment of hepatitis C virus-related liver diseases. Hepatobiliary Pancreat. Dis. Int. 2004, 3, 17-20.
15. Liang, T.J.; Rehermann, B.; Seeff, L.B.; Hoofnagle, J.H. Pathogenesis, natural history, treatment, and prevention of hepatitis C. Ann. Intern. Med. 2000, 132, 296-305.
16. McHutchison, J.G.; Gordon, S.C.; Schiff, E.R.; Shiffman, M.L.; Lee, W.M.; Rustgi, V.K.; Goodman, Z.D.; Ling, M.H.; Cort, S.; Albrecht, J.K. Interferon alfa-2b alone or in combination with ribavirin as initial treatment for chronic hepatitis C. Hepatitis Interventional Therapy Group. N. Engl. J. Med. 1998, 339, 1485-1492.
17. Coon, J.T.; Ernst, E. Complementary and alternative therapies in the treatment of chronic hepatitis C: A systematic review. J. Hepatol. 2004, 40, 491-500.
18. Buckwold, V.E.; Beer, B.E.; Donis, R.O. Bovine viral diarrhea virus as a surrogate model of hepatitis C virus for the evaluation of antiviral agents. Antivir. Res. 2003, 60, 1-15.
19. Romero, M.R.; Serrano, M.A.; Efferth, T.; Alvarez, M.; Marin, J.J. Effect of cantharidin, cephalotaxine and homoharringtonine on "in vitro" models of hepatitis B virus (HBV) and bovine viral diarrhoea virus (BVDV) replication. Planta Med. 2007, 73, 552-558.
20. Kim, T.G.; Kang, S.Y.; Jung, K.K.; Kang, J.H.; Lee, E.; Han, H.M.; Kim, S.H. Antiviral activities of extracts isolated from Terminalis chebula Retz., Sanguisorba officinalis L., Rubus coreanus Miq. and Rheum palmatum L. against hepatitis B virus. Phytother. Res. 2001, 15, 718-720.
21. Pang, R.; Tao, J.; Zhang, S.; Zhu, J.; Yue, X.; Zhao, L.; Ye, P.; Zhu, Y. In vitro anti-hepatitis B virus effect of Hypericum perforatum L. J. Huazhong Univ. Sci. Technol. Med. Sci. 2010, 30, 98-102.
22. Li, Z.; Li, L.J.; Sun, Y.; Li, J. Identification of natural compounds with anti-hepatitis B virus activity from Rheum palmatum L. ethanol extract. Chemotherapy 2007, 53, 320-326.
23. Chiang, L.C.; Ng, L.T.; Liu, L.T.; Shieh, D.E.; Lin, C.C. Cytotoxicity and anti-hepatitis B virus activities of saikosaponins from Bupleurum species. Planta Med. 2003, 69, 705-709.
24. Pink, R.; Hudson, A.; Mouriès, M.A.; Bending, M. Opportunities and challenges in antiparasitic drug discovery. Nat. Rev. Drug Discov. 2005, 4, 727-740.
25. Trouiller, P.; Olliaro, P.; Torreele, E.; Orbinski, J.; Laing, R.; Ford, N. Drug development for neglected diseases: A deficient market and a public-health policy failure. Lancet 2002, 359, 2188-2194.
26. Hoet, S.; Opperdoes, F.; Brun, R.; Quetin-Leclerq, J. Natural products active against African trypanosomes: A step towards new drugs. Nat. Prod. Rep. 2004, 21, 353-364.
27. Räz, B.; Iten, M.; Grether-Bühler, Y.; Kaminsky, R.; Brun, R. The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro. Acta Trop. 1997, 68, 139-147.
28. Kristjanson, P.M.; Swallow, B.M.; Rowlands, G.J.; Kruska, R.L.; de Leeuw, P.N. Measuring the costs of African animal trypanosomiasis, the potential benefits of control and returns to research. Agric. Syst. 1999, 59, 79-98.
29. Fairlamb, A.H. Chemotherapy of human African trypanosomiasis: Current status and future prospects. Trends Parasitol. 2003, 19, 488-494.
30. Fairlamb, A.H. Target discovery and validation with special reference to trypanothione. In Drugs Against Parasitic Diseases: R&D Methodologies and Issues, 1st ed.; Fairlamb, A.H., Ridley, R.G., Vial, H.J., Eds.; WHO TDR: Geneva, Switzerland, 2003; pp. 107-118.
31. Croft, S.L. The current status of antiparasitic chemotherapy. Parasitology 1997, Suppl. 114, 3-15.
32. Ross, C.A.; Sutherland, D.V. Drug resistance in trypanosomatids. In Trypanosomiasis and Leishmaniasis: Biology and Control, 1st ed.; Hide, G., Mottram, J.C., Coombs, G.H., Holmes, P.H., Eds.; CAB international: Wallinford, Oxon, 1997; pp. 259-269.
33. Newman, D.J.; Cragg, G.M.; Snader, K.M. Natural products as sources of new drugs over the period 1981-2002. J. Nat. Prod. 2003, 66, 1022-1037.
34. Legros, D.; Ollivier, G.; Gastellu-Etchegorry, M.; Paquet, C.; Burri, C.; Jannin, J.; Büscher, P. Treatment of human African trypanosomiasis-Present situation and needs for research and development. Lancet Infect. Dis. 2002, 2, 437-440.
35. Brun, R.; Schumacher, R.; Schmid, C.; Kunz, C.; Burri, C. The phenomenon of treatment failures in Human African Trypanosomiasis. Trop. Med. Int. Health. 2001, 6, 906-914.
36. Marin, J.J.; Castaño, B.; Blazquez, A.G.; Rosales, R.; Efferth, T.; Monte, M.J. Strategies for overcoming chemotherapy resistance in enterohepatic tumours. Curr. Mol. Med. 2010, 10, 467-485.
37. Vallejo, M.; Castro, M.A.; Medarde, M.; Macias, R.I.; Romero, M.R.; El-Mir, M.Y.; Monte, M.J.; Briz, O.; Serrano, M.A.; Marin, J.J. Novel bile acid derivatives (BANBs) with cytostatic activity obtained by conjugation of their side chain with nitrogenated bases. Biochem. Pharmacol. 2007, 73, 1394-1404.
38. Baltz, T.; Baltz, D.; Giroud, C.; Crockett, J. Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. EMBO J. 1985, 4, 1273-1277.
39. Kniazeff, A.J.; Wopschall, L.J.; Hopps, H.E.; Morris, C.S. Detection of bovine viruses in fetal bovine serum used in cell culture. In Vitro 1975, 11, 400-403.
40. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
41. Korba, B.E.; Gerin, J.L. Use of a standardized cell culture assay to assess activities of nucleoside analogs against hepatitis B virus replication. Antivir. Res. 1992, 19, 55-70.
42. Fautz, R.; Husein, B.; Hechenberger, C. Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: Rapid colorimetric viability determination for the unscheduled DNA synthesis test (UDS). Mutat. Res. 1991, 253, 173-179.
43. Balasubrahmanyam, A.; Baranwal, V.K.; Lodha, M.L.; Varma, A.; Kapoor, H.C. Purification and properties of growth stage-dependent antiviral proteins from the leaves of Celosia cristata. Plant Sci. 2000, 154, 13-21.
44. Begam, M.; Kumar, S.; Roy, S.; Campanella, J.J.; Kapoor, H.C. Molecular cloning and functional identification of a ribosome inactivating/antiviral protein from leaves of post-flowering stage of Celosia cristata and its expression in E. coli. Phytochemistry 2006, 67, 2441-2449.
45. Begam, M.; Narwal, S.; Roy, S.; Kumar, S.; Lodha, M.L.; Kapoor, H.C. An antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata. Biochemistry (Mosc). 2006, Suppl. 71, 44-48.
46. Gholizadeh, A.; Kohnehrouz, B.B.; Santha, I.M.; Lodha, M.L.; Kapoor, H.C. Cloning and expression of small cDNA fragment encoding strong antiviral peptide from Celosia cristata in Escherichia coli. Biochemistry (Mosc). 2005, 70, 1005-1010.
47. Gholizadeh, A.; Santha, I.M.; Kohnehrouz, B.B.; Lodha, M.L.; Kapoor, H.C. Cystatins may confer viral resistance in plants by inhibition of a virus-induced cell death phenomenon in which cysteine proteinases are active: Cloning and molecular characterization of a cDNA encoding cysteine-proteinase inhibitor (celostatin) from Celosia cristata (crested cock's comb). Biotechnol. Appl. Biochem. 2005, 42, 197-204.
48. Gholizadeh, A.; Kapoor, H.C. Modifications in the purification protocol of Celosia cristata antiviral proteins lead to protein that can be N-terminally sequenced. Protein Pept. Lett. 2004, 11, 551-561.
49. Baranwal, V.K.; Tumer, N.E.; Kapoor, H.C. Depurination of ribosomal RNA and inhibition of viral RNA translation by an antiviral protein of Celosia cristata. Indian J. Exp. Biol. 2002, 40, 1195-1197.
50. Lin, Y.L.; Shen, C.C.; Huang, Y.J.; Chang, Y.Y. Homoflavonoids from Ophioglossum Petiolatum. J. Nat. Prod. 2005, 68, 381-384.
51. Hayashi, K.; Kamiya, M.; Hayashi, T. Virucidal effects of the steam distillate from Houttuynia cordata and its components on HSV-1, influenza virus, and HIV. Planta Med. 1995, 61, 237-241.
52. Lin, T.Y.; Liu, Y.C.; Jheng, J.R.; Tsai, H.P.; Jan, J.T.; Wong, W.R.; Horng, J.T. Anti-enterovirus 71 activity screening of chinese herbs with anti-infection and inflammation activities. Am. J. Chin. Med. 2009, 37, 143-158.
53. Lau, K.M.; Lee, K.M.; Koon, C.M.; Cheung, C.S.; Lau, C.P.; Ho, H.M.; Lee, M.Y.; Au, S.W.; Cheng, C.H.; Lau, C.B.; et al. Immunomodulatory and anti-SARS activities of Houttuynia cordata. J. Ethnopharmacol. 2008, 118, 79-85.
54. Ren, X.; Sui, X.; Yin, J. The effect of Houttuynia cordata injection on pseudorabies herpesvirus (PrV) infection in vitro. Pharm. Biol. 2011, 49, 161-166.
55. Chou, S.C.; Su, C.R.; Ku, Y.C.; Wu, T.S. The constituents and their bioactivities of Houttuynia cordata. Chem. Pharm. Bull. (Tokyo). 2009, 57, 1227-1230.
56. Gonzalez, O.; Fontanes, V.; Raychaudhuri, S.; Loo, R.; Loo, J.; Arumugaswami, V.; Sun, R.; Dasgupta, A.; French, S.W. The heat shock protein inhibitor Quercetin attenuates hepatitis C virus production. Hepatology. 2009, 50, 1756-1764.
57. Choi, H.J.; Song, J.H.; Park, K.S.; Kwon, D.H. Inhibitory effects of quercetin 3-rhamnoside on influenza A virus replication. Eur. J. Pharm. Sci. 2009, 37, 329-333.
58. Jung, H.J.; Sung, W.S.; Yeo, S.H.; Kim, H.S.; Lee, I.S.; Woo, E.R.; Lee, D.G. Antifungal effect of amentoflavone derived from Selaginella tamariscina. Arch. Pharm. Res. 2006, 29, 746-751.
59. Lee, J.; Choi, Y.; Woo, E.R.; Lee, D.G. Antibacterial and synergistic activity of isocryptomerin isolated from Selaginella tamariscina. J. Microbiol. Biotechnol. 2009, 19, 204-207.
60. Lee, J.; Choi, Y.; Woo, E.R.; Lee, D.G. Isocryptomerin, a novel membrane-active antifungal compound from Selaginella tamariscina. Biochem. Biophys. Res. Commun. 2009, 379, 676-680.
61. Lee, J.S.; Lee, M.S.; Oh, W.K.; Sul, J.Y. Fatty acid synthase inhibition by amentoflavone induces apoptosis and antiproliferation in human breast cancer cells. Biol. Pharm. Bull. 2009, 32, 1427-1432.
62. Gao, L.L.; Yin, S.L.; Li, Z.L.; Sha, Y.; Pei, Y.H.; Shi, G.; Jing, Y.K.; Hua, H.M. Three novel sterols isolated from Selaginella tamariscina with antiproliferative activity in leukemia cells. Planta Med. 2007, 73, 1112-1115.
63. Lee, C.W.; Choi, H.J.; Kim, H.S.; Kim, D.H.; Chang, I.S.; Moon, H.T.; Lee, S.Y.; Oh, W.K.; Woo, E.R. Biflavonoids isolated from Selaginella tamariscina regulate the expression of matrix metalloproteinase in human skin fibroblasts. Bioorg. Med. Chem. 2008, 16, 732-738.
64. Rao, K.; Ch, B.; Narasu, L.M.; Giri, A. Antibacterial activity of Alpinia galanga (L) Willd crude extracts. Appl. Biochem. Biotechnol. 2010, 162, 871-884.
65. Weerakkody, N.S.; Caffin, N.; Lambert, L.K.; Turner, M.S.; Dykes, G.A. Synergistic antimicrobial activity of galangal (Alpinia galanga), rosemary (Rosmarinus officinalis) and lemon iron bark (Eucalyptus staigerana) extracts. J. Sci. Food Agric. 2011, 91, 461-468.
66. Kaur, A.; Singh, R.; Dey, C.S.; Sharma, S.S.; Bhutani, K.K.; Singh, I.P. Antileishmanial phenylpropanoids from Alpinia galanga (Linn.) Willd. Indian J. Exp. Biol. 2010, 48, 314-317.
67. Tamura, S.; Shiomi, A.; Kimura, T.; Murakami, N. Halogenated analogs of 1'-acetoxychavicol acetate, Rev-export inhibitor from Alpinia galanga, designed from mechanism of action. Bioorg. Med. Chem. Lett. 2010, 20, 2082-2085.
68. Tamura, S.; Shiomi, A.; Kaneko, M.; Ye, Y.; Yoshida, M.; Yoshikawa, M.; Kimura, T.; Kobayashi, M.; Murakami, N. New Rev-export inhibitor from Alpinia galanga and structure-activity relationship. Bioorg. Med. Chem. Lett. 2009, 19, 2555-2557.
69. Liu, Y.; Murakami, N.; Zhang, S.; Xu, T. Structure-activity relationships of 1'-acetoxychavicol acetate homologues as new nuclear export signal inhibitors. Pharmazie 2007, 62, 659-662.
70. Ye, Y.; Li, B. 1'S-1'-acetoxychavicol acetate isolated from Alpinia galanga inhibits human immunodeficiency virus type 1 replication by blocking Rev transport. J. Gen. Virol. 2006, 87, 2047-2053.
71. Miyazawa, M.; Nakamura, Y.; Ishikawa, Y. Insecticidal diarylheptanoid from Alpinia oxyphylla against larvae of Drosophila melanogaster. Nat. Prod. Lett. 2001, 15, 75-79.
72. Shin, T.Y.; Won, J.H.; Kim, H.M.; Kim, S.H. Effect of Alpinia oxyphylla fruit extract on compound 48/80-induced anaphylactic reactions. Am. J. Chin. Med. 2001, 29, 293-302.
73. He, Z.H.; Ge, W.; Yue, G.G.; Lau, C.B.; He, M.F.; But, P.P. Anti-angiogenic effects of the fruit of Alpinia oxyphylla. J. Ethnopharmacol. 2010, 132, 443-449.
74. Li, G.L.; Zhu, D.Y. Two dichromenes from Evodia lepta. J. Asian Nat. Prod. Res. 1999, 1, 337-341.
75. Fiore, C.; Eisenhut, M.; Krausse, R.; Ragazzi, E.; Pellati, D.; Armanini, D.; Bielenberg, J. Antiviral effects of Glycyrrhiza species. Phytother. Res. 2008, 22, 141-148.
76. Zhang, Y.; Chen, Y.; Fan, C.; Ye, W.; Luo, J. Two new iridoid glucosides from Hedyotis diffusa. Fitoterapia 2010, 81, 515-517.
77. Li, C.; Xue, X.; Zhou, D.; Zhang, F.; Xu, Q.; Ren, L.; Liang, X. Analysis of iridoid glucosides in Hedyotis diffusa by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry. J. Pharm. Biomed. Anal. 2008, 48, 205-211.
78. Lin, C.C.; Ng, L.T.; Yang, J.J.; Hsu, Y.F. Anti-inflammatory and hepatoprotective activity of peh-hue-juwa-chi-cao in male rats. Am. J. Chin. Med. 2002, 30, 225-234.
79. Jiang, R.W.; Zhou, J.R.; Hon, P.M.; Li, S.L.; Zhou, Y.; Li, L.L.; Ye, W.C.; Xu, H.X.; Shaw, P.C.; But, P.P. Lignans from Dysosma versipellis with inhibitory effects on prostate cancer cell lines. J. Nat. Prod. 2007, 70, 283-286.
80. Mulyaningsih, S.; Youns, M.; El-Readi, M.Z.; Ashour, M.L.; Nibret, E.; Sporer, F.; Herrmann, F.; Reichling, J.; Wink, M. Biological activity of the essential oil of Kadsura longipedunculata (Schisandraceae) and its major components. J. Pharm. Pharmacol. 2010, 62, 1037-1044.
81. Kim, H.J.; Chang, E.J.; Cho, S.H.; Chung, S.K.; Park, H.D.; Choi, S.W. Antioxidative activity of resveratrol and its derivatives isolated from seeds of Paeonia lactiflora. Biosci. Biotechnol. Biochem. 2002, 66, 1990-1993.
82. Fuggetta, M.P.; D'Atri, S.; Lanzilli, G.; Tricarico, M.; Cannavò, E.; Zambruno, G.; Falchetti, R.; Ravagnan, G. In vitro antitumour activity of resveratrol in human melanoma cells sensitive or resistant to temozolomide. Melanoma Res. 2004, 14, 189-196.
83. Kang, J.H.; Park, Y.H.; Choi, S.W.; Yang, E.K.; Lee, W.J. Resveratrol derivatives potently induce apoptosis in human promyelocytic leukemia cells. Exp. Mol. Med. 2003, 35, 467-474.
84. Kim, H.J.; Chang, E.J.; Bae, S.J.; Shim, S.M.; Park, H.D.; Rhee, C.H.; Park, J.H.; Choi, S.W. Cytotoxic and antimutagenic stilbenes from seeds of Paeonia lactiflora. Arch. Pharm. Res. 2002, 25, 293-299.
85. Loizzo, M.R.; Tundis, R.; Menichini, F.; Saab, A.M.; Statti, G.A.; Menichini, F. Antiproliferative effects of essential oils and their major constituents in human renal adenocarcinoma and amelanotic melanoma cells. Cell Prolif. 2008, 41, 1002-1012.
86. Yang, L.Q.; Singh, M.; Yap, E.H.; Ng, G.C.; Xu, H.X.; Sim, K.Y. In vitro response of Blastocystis hominis against traditional Chinese medicine. J. Ethnopharmacol. 1996, 55, 35-42.
87. Hirano, H.; Osawa, E.; Yamaoka, Y.; Yokoi, T. Gastric-mucous membrane protection activity of coptisine derivatives. Biol. Pharm. Bull. 2001, 24, 1277-1281.
88. Joshi, P.V.; Shirkhedkar, A.A.; Prakash, K.; Maheshwari, V.L. Antidiarrheal activity, chemical and toxicity profile of Berberis aristata. Pharm. Biol. 2011, in press.
89. Liu, X.; Li, G.; Zhu, H.; Huang, L.; Liu, Y.; Ma, C.; Qin, C. Beneficial effect of berberine on hepatic insulin resistance in diabetic hamsters possibly involves in SREBPs, LXRalpha and PPARalpha transcriptional programs. Endocr. J. 2011, in press.
90. Dvorak, Z. Berberine: A multipotent remedy with unknown cellular target? Poult. Sci. 2010, 89, 1787.
91. Patil, J.B.; Kim, J.; Jayaprakasha, G.K. Berberine induces apoptosis in breast cancer cells (MCF-7) through mitochondrial-dependent pathway. Eur. J. Pharmacol. 2010, 645, 70-78.
92. Lin, C.C.; Ng, L.T.; Hsu, F.F.; Shieh, D.E.; Chiang, L.C. Cytotoxic effects of Coptis chinensis and Epimedium sagittatum extracts and their major constituents (berberine, coptisine and icariin) on hepatoma and leukaemia cell growth. Clin. Exp. Pharmacol. Physiol. 2004, 31, 65-69.
93. Miyazawa, M.; Nakamura, Y.; Ishikawa, Y. Insecticidal sesquiterpene from Alpinia oxyphylla against Drosophila melanogaster. J. Agric. Food Chem. 2000, 48, 3639-3641.
94. Pu, J.X.; Gao, X.M.; Lei, C.; Xiao, W.L.; Wang, R.R.; Yang, L.B.; Zhao, Y.; Li, L.M.; Huang, S.X.; Zheng, Y.T.; Sun, H.D. Three new compounds from Kadsura longipedunculata. Chem. Pharm. Bull. (Tokyo). 2008, 56, 1143-1146.
95. Kamath, S.; Skeels, M.; Pai, A. Significant differences in alkaloid content of Coptis chinensis (Huanglian), from its related American species. Chin. Med. 2009, 4, doi:10.1186/1749-8546-4-17.
96. Rosenkranz, V.; Wink, M. Alkaloids induce programmed cell death in bloodstream forms of trypanosomes (Trypanosoma b. brucei). Molecules 2008, 13, 2462-2473.
97. Merschjohann, K.; Sporer, F.; Steverding, D.; Wink, M. In vitro effect of alkaloids on bloodstream forms of Trypanosoma brucei and T. congolense. Planta Med. 2001, 67, 623-627.
98. Rosenkranz, V.; Wink, M. Alkaloids induce programmed cell death in bloodstream forms of trypanosomes (Trypanosoma b. brucei). Molecules. 2008, 13, 2462-2473.
99. Freiburghaus, F.; Kaminsky, R.; Nkunya, M.H.H.; Brun, R. Evaluation of African medicinal plants for their in vitro trypanocidal activity. J. Ethnopharmacol. 1996, 55, 1-11.