Mediator acts upstream of the transcriptional activator Gal4

PLoS Biology

Volumn 10, Issue 3, 2012, Pages

  Ang, Keven ^a   Ee, Gary ^a   Ang, Edwin ^a   Koh, Elvin ^a   Siew, Wee Leng ^a   Chan, Yu Mun ^a   Nur, Sabrina ^a   Tan, Yee Sun ^a   Lehming, Norbert ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

F BOX PROTEIN; GAL80 PROTEIN; GALACTOSE; GLUCOSE; PROTEIN; PROTEIN KINASE; PROTEIN KINASE SNF1; RNA POLYMERASE; TRANSCRIPTION FACTOR GAL4; UBIQUITIN; UBIQUITIN PROTEIN LIGASE E3; UNCLASSIFIED DRUG; CYCLINE; DNA BINDING PROTEIN; GAL4 PROTEIN, S CEREVISIAE; GAL80 PROTEIN, S CEREVISIAE; MDM30 PROTEIN, S CEREVISIAE; MED21 PROTEIN, HUMAN; MEDIATOR COMPLEX; REPRESSOR PROTEIN; S PHASE KINASE ASSOCIATED PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; SKP1 PROTEIN, HUMAN; SSN8 PROTEIN, S CEREVISIAE; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; GENE CONTROL; GENE DELETION; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; GENETIC CODE; MUTAGENESIS; NONHUMAN; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; CULTURE MEDIUM; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; HELA CELL; HUMAN; IMMUNOPRECIPITATION; METABOLISM; PROTEIN BINDING; PROTEIN STABILITY; SIGNAL TRANSDUCTION; TRANSCRIPTION INITIATION;

CULTURE MEDIA; CYCLINS; DNA-BINDING PROTEINS; F-BOX PROTEINS; GALACTOSE; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; MEDIATOR COMPLEX; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PROTEIN STABILITY; PROTEOLYSIS; REPRESSOR PROTEINS; S-PHASE KINASE-ASSOCIATED PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION; UBIQUITIN;

EID: 84858963661     PISSN: 15449173     EISSN: 15457885     Source Type: Journal    
DOI: 10.1371/journal.pbio.1001290     Document Type: Article

References (58)

1. Ptashne M, (2005) Regulation of transcription, from lambda to eukaryotes. Trends Biochem Sci 30: 275-279.
2. Bryant G. O, Prabhu V, Floer M, Wang X, Spagna D, et al. (2008) Activator control of nucleosome occupancy in activation and repression of transcription. PLoS Biol 6: 2928-2939 doi:10.1371/journal.pbio.0060317.
3. Floer M, Wang X, Prabhu V, Berrozpe G, Narayan S, et al. (2010) A RSC/nucleosome complex determines chromatin architecture and facilitates activator binding. Cell 141: 407-418.
4. Kadosh D, Struhl K, (1997) Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters. Cell 89: 365-371.
5. Jiang C, Pugh B. F, (2009) Nucleosome positioning and gene regulation: advances through genomics. Nat Rev Genet 10: 161-172.
6. Wellhausen A, Lehming N, (1999) Analysis of the in vivo interaction between a basic repressor and an acidic activator. FEBS Lett 453: 299-304.
7. Santangelo G. M, (2006) Glucose signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 70: 253-282.
8. Maniatis T, Reed R, (2002) An extensive network of coupling among gene expression machines. Nature 416: 499-506.
9. Traven A, Jelicic B, Sopta M, (2006) Yeast Gal4, a transcriptional paradigm revisited. EMBO Rep 7: 496-499.
10. Diep C. Q, Tao X, Pilauri V, Losiewicz M, Blank T. E, et al. (2008) Genetic evidence for sites of interaction between the Gal3 and Gal80 proteins of the Saccharomyces cerevisiae GAL gene switch. Genetics 178: 725-736.
11. Treitel M. A, Kuchin S, Carlson M, (1998) Snf1 protein kinase regulates phosphorylation of the Mig1 repressor in Saccharomyces cerevisiae. Mol Cell Biol 18: 6273-6280.
12. De Vit M. J, Waddle J. A, Johnston M, (1997) Regulated nuclear translocation of the Mig1 glucose repressor. Mol Biol Cell 8: 1603-1618.
13. Jiang F, Frey B. R, Evans M. L, Friel J. C, Hopper J. E, (2009) Gene activation by dissociation of an inhibitor from a transcriptional activation domain. Mol Cell Biol 29: 5604-5610.
14. Sellick C. A, Reece R. J, (2005) Eukaryotic transcription factors as direct nutrient sensors. Trends Biochem Sci 30: 405-412.
15. Muratani M, Tansey W. P, (2003) How the ubiquitin-proteasome system controls transcription. Nat Rev Mol Cell Biol 4: 1-10.
16. Hu R. G, Wang H, Xia Z, Varshavsky A, (2008) The N-end rule pathway is a sensor of heme. Proc Natl Acad Sci U S A 105: 76-81.
17. Lipford J. R, Smith G. T, Chi Y, Deshaies R. J, (2005) A putative stimulatory role for activator turnover in gene expression. Nature 438: 113-116.
18. Varshavsky A, (1997) The ubiquitin system. Trends Biochem Sci 22: 383-387.
19. Skowyra D, Craig K. L, Tyers M, Elledge S. J, Harper J. W, (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91: 209-219.
20. Muratani M, Kung C, Shokat K. M, Tansey W. P, (2005) The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell 120: 887-899.
21. Nalley K, Johnston S. A, Kodadek T, (2006) Proteolytic turnover of the Gal4 transcription factor is not required for function in vivo. Nature 442: 1054-1057.
22. Collins G. A, Lipford J. R, Deshaies R. J, Tansey W. P, (2009) Gal4 turnover and transcription activation. Nature 461: E7.
23. Nalley K, Johnston S. A, Kodadek T, (2009) Nalleyet al. reply. Nature 461: E8.
24. Gonzalez F, Delahodde A, Kodadek T, Johnston S. A, (2002) Recruitment of a 19S proteasome subcomplex to an activated promoter. Science 296: 548-550.
25. Archer C. T, Delahodde A, Gonzalez F, Johnston S. A, Kodadek T, (2008) Activation domain-dependent monoubiquitylation of Gal4 protein is essential for promoter binding in vivo. J Biol Chem 283: 12614-12623.
26. Archer C. T, Kodadek T, (2010) The hydrophobic patch of ubiquitin is required to protect transactivator-promoter complexes from destabilization by the proteasomal ATPases. Nucleic Acids Res 38: 789-796.
27. Wang X, Muratani M, Tansey W. P, Ptashne M, (2010) Proteolytic instability and the action of nonclassical transcriptional activators. Curr Biol 20: 868-871.
28. Malik S, Roeder R. G, (2010) The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation. Nat Rev Genet 11: 761-772.
29. Kornberg R. D, (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30: 235-239.
30. Holstege F. C, Jennings E. G, Wyrick J. J, Lee T. I, Hengartner C. J, et al. (1998) Dissecting the regulatory circuitry of a eukaryotic genome. Cell 95: 717-728.
31. Taatjes D. J, (2010) The human Mediator complex: a versatile, genome-wide regulator of transcription. Trends Biochem Sci 35: 315-322.
32. Kagey M. H, Newman J. J, Bilodeau S, Zhan Y, Orlando D. A, et al. (2010) Mediator and cohesin connect gene expression and chromatin architecture. Nature 467: 430-435.
33. Takahashi H, Parmely T. J, Sato S, Tomomori-Sato C, Banks C. A, et al. (2011) Human mediator subunit MED26 functions as a docking site for transcription elongation factors. Cell 146: 92-104.
34. Meyer K. D, Lin S. C, Bernecky C, Gao Y, Taatjes D. J, (2010) p53 activates transcription by directing structural shifts in Mediator. Nat Struct Mol Biol 17: 753-760.
35. Ding N, Zhou H, Esteve P. O, Chin H. G, Kim S, et al. (2008) Mediator links epigenetic silencing of neuronal gene expression with x-linked mental retardation. Mol Cell 31: 347-359.
36. Nasmyth K. A, Reed S. I, (1980) Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci U S A 77: 2119-2123.
37. Lim M. K, Siew W. L, Zhao J, Tay Y. W, Ang E, et al. (2011) Galactose induction of the GAL1 gene requires conditional degradation of the Mig2 repressor. Biochemical J 435: 641-649.
38. Keleher C. A, Redd M. J, Schultz J, Carlson M, Johnson A. D, (1992) Ssn6-Tup1 is a general repressor of transcription in yeast. Cell 68: 709-719.
39. Treitel M. A, Carlson M, (1995) Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein. Proc Natl Acad Sci U S A 92: 3132-3136.
40. Kitagawa K, Skowyra D, Elledge S. J, Harper J. W, Hieter P, (1999) SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. Mol Cell 4: 21-33.
41. Chew B. S, Siew W. L, Xiao B, Lehming N, (2010) Transcriptional activation requires protection of the TATA-binding protein Tbp1 by the ubiquitin-specific protease Ubp3. Biochemical J 431: 391-399.
42. Li Y, Chen G, Liu W, (2010) Alterations in the interaction between GAL4 and GAL80 affect regulation of the yeast GAL regulon mediated by the F box protein Dsg1. Curr Microbiol 61: 210-216.
43. Lim M. K, Tang V, Le Saux A, Schüller J, Bongards C, et al. (2007) Gal11p dosage-compensates transcriptional activator deletions via Taf14p. J Mol Biol 374: 9-23.
44. Kumar P. R, Yu Y, Sternglanz R, Johnston S. A, Joshua-Tor L, (2008) NADP regulates the yeast GAL induction system. Science 319: 1090-1092.
45. Gromöller A, Lehming N, (2000) Srb7p is essential for the activation of a subset of genes. FEBS Lett 484: 48-54.
46. Gromöller A, Lehming N, (2000) Srb7p is a physical and physiological target of Tup1p. EMBO J 19: 6845-6852.
47. Lehming N, (2002) Analysis of protein-protein proximities using the split-ubiquitin system. Brief Funct Genomic Proteomic 1: 230-238.
48. Reichel C, Johnsson N, (2005) The split-ubiquitin sensor: measuring interactions and conformational alterations of proteins in vivo. Methods Enzymol 399: 757-776.
49. Rojo-Niersbach E, Morley D, Heck S, Lehming N, (2000) A new method for the selection of protein interactions in mammalian cells. Biochemical J 348: 585-590.
50. Kuchin S, Treich I, Carlson M, (2000) A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A 97: 7916-7920.
51. Carlson M, Osmond B. C, Neigeborn L, Botstein D, (1984) A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics 107: 19-32.
52. Ansari A. Z, Koh S. S, Zaman Z, Bongards C, Lehming N, et al. (2002) Transcriptional activating regions target a cyclin-dependent kinase. Proc Natl Acad Sci U S A 99: 14706-14709.
53. Finley D, Sadis S, Monia B. P, Boucher P, Ecker D. J, et al. (1994) Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol Cell Biol 14: 5501-5509.
54. Gietz R. D, Sugino A, (1988) New yeast- Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
55. Sikorski R. S, Hieter P, (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
56. Alani E, Cao L, Kleckner N, (1987) A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545.
57. Laser H, Bongards C, Schüller J, Heck S, Johnsson N, et al. (2000) A new screen for protein interactions reveals that the Saccharomyces cerevisiae high mobility group proteins Nhp6A/B are involved in the regulation of the GAL1 promoter. Proc Natl Acad Sci U S A 97: 13732-13737.
58. Dohmen R. J, Stappen R, McGrath J. P, Forrova H, Kolarov J, et al. (1995) An essential yeast gene encoding a homolog of ubiquitin-activating enzyme. J Biol Chem 270: 18099-18109.