Relative quantitation of proteins in expressed prostatic secretion with a stable isotope labeled secretome standard

Journal of Proteome Research

Volumn 11, Issue 2, 2012, Pages 1089-1099

  Zhao, Ting ^a,b   Zeng, Xuemei ^b   Bateman, Nicholas W ^a   Sun, Mai ^b   Teng, Pang Ning ^a,b   Bigbee, William L ^a,b   Dhir, Rajiv ^a   Nelson, Joel B ^a   Conrads, Thomas P ^a,b,c   Hood, Brian L ^a,b,c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH CANCER INSTITUTE   (United States)

^c BETTY AND GUY BEATTY CENTER FOR INTEGRATED RESEARCH   (United States)

Author keywords

expressed prostatic secretion; mass spectrometry; prostate cancer; proteomics; SILAC

Indexed keywords

ALPHA ENOLASE; AMYLOID BETA A4 PROTEIN; BETA 2 MICROGLOBULIN; BRAIN ACID SOLUBLE PROTEIN 1; CALMODULIN; CATHEPSIN D; CATHEPSIN X; CD9 ANTIGEN; CLUSTERIN; CYSTATIN C; DYSTROGLYCAN; FIBRONECTIN; FILAMIN A; FOLLISTATIN RELATED PROTEIN 1; GALECTIN 3 BINDING PROTEIN; GLUTATHIONE TRANSFERASE P1; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GRANULIN; HEAT SHOCK PROTEIN 90 ALPHA; L LACTATE DEHYDROGENASE A CHAIN; LATENT TRANSFORMING GROWTH FACTOR BETA BINDING PROTEIN 1; LIPOCORTIN 2; NEUROPILIN 1; PENTRAXIN RELATED PROTEIN 3; PEPTIDES AND PROTEINS; PEROXIREDOXIN 1; PEROXIREDOXIN 2; PHOSPHOGLYCERATE KINASE 1; PROFILIN I; RIBONUCLEASE 4; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CANCER CELL; CANCER PATIENT; CONTROLLED STUDY; CULTURE MEDIUM; EXPRESSED PROSTATIC SECRETION; HUMAN; HUMAN CELL; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MALE; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE SECRETION; PROTEIN ANALYSIS; STABLE ISOTOPE LABELED PROTEOME STANDARD; STANDARD; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; BODILY SECRETIONS; CELL LINE, TUMOR; HUMANS; ISOTOPE LABELING; MALE; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROSTATE; PROSTATIC NEOPLASMS; PROTEIN INTERACTION MAPS; PROTEINS; PROTEOME; PROTEOMICS; REFERENCE STANDARDS;

EID: 84856669219     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr200829f     Document Type: Article

References (77)

1. Jemal, A.; Siegel, R.; Xu, J.; Ward, E. Cancer Statistics, 2010 Ca-Cancer J. Clin. 2010, 60 (5) 277-300
2. Lilja, H.; Ulmert, D.; Vickers, A. J. Prostate-specific antigen and prostate cancer: prediction, detection and monitoring Nat. Rev. Cancer 2008, 8 (4) 268-78
3. Shariat, S. F.; Scardino, P. T.; Lilja, H. Screening for prostate cancer: an update Can. J. Urol. 2008, 15 (6) 4363-74
4. Piersma, S. R.; Fiedler, U.; Span, S.; Lingnau, A.; Pham, T. V.; Hoffmann, S.; Kubbutat, M. H. G.; Jiménez, C. R. Workflow comparison for label-free, quantitative secretome proteomics for cancer biomarker discovery: method evaluation, differential analysis, and verification in serum J. Proteome Res. 2010, 9 (4) 1913-22
5. Chenau, J.; Michelland, S.; de Fraipont, F.; Josserand, V.; Coll, J. L.; Favrot, M. C.; Seve, M. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells J. Proteome Res. 2009, 8 (10) 4579-91
6. Lawlor, K.; Nazarian, A.; Lacomis, L.; Tempst, P.; Villanueva, J. Pathway-based biomarker search by high-throughput proteomics profiling of secretomes J. Proteome Res. 2009, 8 (3) 1489-503
7. Xue, H.; Lu, B.; Lai, M. The cancer secretome: a reservoir of biomarkers J. Transl. Med. 2008, 6 (1) 52
8. Greenbaum, D.; Luscombe, N. M.; Jansen, R.; Qian, J.; Gerstein, M. Interrelating different types of genomic data, from proteome to secretome: 'oming in on function Genome Res. 2001, 11 (9) 1463-8
9. Li, R.; Guo, Y.; Han, B. M.; Yan, X.; Utleg, A. G.; Li, W.; Tu, L. C.; Wang, J.; Hood, L.; Xia, S.; Lin, B. Proteomics cataloging analysis of human expressed prostatic secretions reveals rich source of biomarker candidates Proteomics Clin. Appl. 2008, 2 (4) 543-55
10. Drake, R. R.; Elschenbroich, S.; Lopez-Perez, O.; Kim, Y.; Ignatchenko, V.; Ignatchenko, A.; Nyalwidhe, J. O.; Basu, G.; Wilkins, C. E.; Gjurich, B.; Lance, R. S.; Semmes, O. J.; Medin, J. A.; Kislinger, T. In-depth proteomic analyses of direct expressed prostatic secretions J. Proteome Res. 2010, 9 (5) 2109-16
11. Ong, S. E.; Foster, L. J.; Mann, M. Mass spectrometric-based approaches in quantitative proteomics Methods 2003, 29 (2) 124-30
12. Ong, S. E.; Mann, M. Mass spectrometry-based proteomics turns quantitative Nat. Chem. Biol. 2005, 1 (5) 252-62
13. Vaudel, M.; Sickmann, A.; Martens, L. Peptide and protein quantification: A map of the minefield Proteomics 2010, 10 (4) 650-70
14. Ong, S. E.; Blagoev, B.; Kratchmarova, I.; Kristensen, D. B.; Steen, H.; Pandey, A.; Mann, M. Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics Mol. Cell. Proteomics 2002, 1, 376-86
15. Ong, S. E.; Mann, M. A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) Nat. Protoc. 2007, 1 (6) 2650-60
16. Ishihama, Y.; Sato, T.; Tabata, T.; Miyamoto, N.; Sagane, K.; Nagasu, T.; Oda, Y. Quantitative mouse brain proteomics using culture-derived isotope tags as internal standards Nat. Biotechnol. 2005, 23 (5) 617-21
17. Yan, Y.; Weaver, V. M.; Blair, I. A. Analysis of protein expression during oxidative stress in breast epithelial cells using a stable isotope labeled proteome internal standard J. Proteome Res. 2005, 4 (6) 2007-14
18. Yu, K. H.; Barry, C. G.; Austin, D.; Busch, C. M.; Sangar, V.; Rustgi, A. K.; Blair, I. A. Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery J. Proteome Res. 2009, 8 (3) 1565-76
19. Rangiah, K.; Tippornwong, M.; Sangar, V.; Austin, D.; Tétreault, M.-P.; Rustgi, A. K.; Blair, I. A.; Yu, K. H. Differential secreted proteome approach in murine model for candidate biomarker discovery in colon cancer J. Proteome Res. 2009, 8 (11) 5153-64
20. Shah, S. J.; Yu, K. H.; Sangar, V.; Parry, S. I.; Blair, I. A. Identification and quantification of preterm birth biomarkers in human cervicovaginal fluid by liquid chromatography/tandem mass spectrometry J. Proteome Res. 2009, 8 (5) 2407-17
21. Geiger, T.; Cox, J.; Ostasiewicz, P.; Wisniewski, J. R.; Mann, M. Super-SILAC mix for quantitative proteomics of human tumor tissue Nat. Methods 2010, 7 (5) 383-5
22. Elias, J. E.; Gygi, S. P. Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry Nat. Methods 2007, 4 (3) 207-14
23. Zeng, X.; Hood, B. L.; Sun, M.; Conrads, T. P.; Day, R. S.; Weissfeld, J. L.; Siegfried, J. M.; Bigbee, W. L. Lung cancer serum biomarker discovery using glycoprotein capture and liquid chromatography mass spectrometry J. Proteome Res. 2010, 9 (12) 6440-9
24. Albrecht, M.; Renneberg, H.; Wennemuth, G.; Möschler, O.; Janssen, M.; Aumüller, G.; Konrad, L. Fibronectin in human prostatic cells in vivo and in vitro: expression, distribution, and pathological significance Histochem. Cell Biol. 1999, 112 (1) 51-61
25. Hu, S.; Delorme, N.; Liu, Z.; Velasco-Gonzalez, C.; Garai, J.; Pullikuth, A.; Koochekpour, S. Prosaposin down-modulation decreases metastatic prostate cancer cell adhesion, migration, and invasion Mol. Cancer 2010, 9, 30
26. O'Brien, J.; Carson, G.; Seo, H.; Hiraiwa, M.; Weiler, S.; Tomich, J.; Barranger, J.; Kahn, M.; Azuma, N.; Kishimoto, Y. Identification of the neurotrophic factor sequence of prosaposin FASEB J. 1995, 9 (8) 681-5
27. Gava, L. M.; Ramos, C. H. I. Human 90 kDa heat shock protein Hsp90 as a target for cancer therapeutics Curr. Chem. Biol. 2009, 3, 330-41
28. Isaacs, J. S.; Xu, W.; Neckers, L. Heat shock protein 90 as a molecular target for cancer therapeutics Cancer Cell 2003, 3 (3) 213-7
29. Vanaja, D. K.; Mitchell, S. H.; Toft, D. O.; Young, C. Y. Effect of geldanamycin on androgen receptor function and stability Cell Stress Chaperones 2002, 7 (1) 55-64
30. Lamoureux, F.; Thomas, C.; Yin, M. J.; Kuruma, H.; Fazli, L.; Gleave, M. E.; Zoubeidi, A. A novel HSP90 inhibitor delays castrate-resistant prostate cancer without altering serum PSA levels and Inhibits osteoclastogenesis Clin. Cancer Res. 2011, 17 (8) 2301-13
31. Bateman, A.; Bennett, H. P. J. The granulin gene family: from cancer to dementia BioEssays 2009, 31 (11) 1245-54
32. Pan, C. X.; Kinch, M. S.; Kiener, P. A.; Langermann, S.; Serrero, G.; Sun, L.; Corvera, J.; Sweeney, C. J.; Li, L.; Zhang, S.; Baldridge, L. A.; Jones, T. D.; Koch, M. O.; Ulbright, T. M.; Eble, J. N.; Cheng, L. PC cell-derived growth factor expression in prostatic intraepithelial neoplasia and prostatic adenocarcinoma Clin. Cancer Res. 2004, 10 (4) 1333-7
33. Liu, K. J.; Shih, N. Y. The role of enolase in tissue invasion and metastasis of pathogens and tumor cells J. Cancer Mol. 2007, 3 (2) 45-8
34. Diamandis, E. P.; Goodglick, L.; Planque, C.; Thornquist, M. D. Pentraxin-3 is a novel biomarker of lung carcinoma Clin. Cancer Res. 2011, 17 (8) 2395-9
35. Ravenna, L.; Sale, P.; Di Vito, M.; Russo, A.; Salvatori, L.; Tafani, M.; Mari, E.; Sentinelli, S.; Petrangeli, E.; Gallucci, M.; Di Silverio, F.; Russo, M. A. Up-regulation of the inflammatory-reparative phenotype in human prostate carcinoma Prostate 2009, 69 (11) 1245-55
36. Gross, M.; Top, I.; Laux, I.; Katz, J.; Curran, J.; Tindell, C.; Agus, D. β-2-Microglobulin is an androgen-regulated secreted protein elevated in serum of patients with advanced prostate cancer Clin. Cancer Res. 2007, 13 (7) 1979-86
37. Garraway, I. P.; Seligson, D.; Said, J.; Horvath, S.; Reiter, R. E. Trefoil factor 3 is overexpressed in human prostate cancer Prostate 2004, 61 (3) 209-14
38. Wang, J.; Ying, G.; Wang, J.; Jung, Y.; Lu, J.; Zhu, J.; Pienta, K. J.; Taichman, R. S. Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression Cancer Res. 2010, 70 (2) 471-80
39. Thim, L. Trefoil peptides: from structure to function Cell. Mol. Life Sci. 1997, 53 (11) 888-903
40. Vestergaard, E. M.; Borre, M.; Poulsen, S. S.; Nexø, E.; Tørring, N. Plasma levels of trefoil factors are increased in patients with advanced prostate cancer Clin. Cancer Res. 2006, 12 (3) 807-12
41. Waisman, D. M. Annexin II tetramer: structure and function Mol. Cell. Biochem. 1995, 149 (1) 301-22
42. Rothhut, B. Participation of annexins in protein phosphorylation Cell. Mol. Life Sci. 1997, 53 (6) 522-6
43. Yee, D. S.; Narula, N.; Ramzy, I.; Boker, J.; Ahlering, T. E.; Skarecky, D. W.; Ornstein, D. K. Reduced annexin II protein expression in high-grade prostatic intraepithelial neoplasia and prostate cancer Arch. Pathol. Lab. Med. 2007, 131 (6) 902-8
44. Downing, S. R.; Russell, P. J.; Jackson, P. Alterations of p53 are common in early stage prostate cancer Can. J. Urol. 2003, 10 (4) 1924-33
45. Sun, C.; Shi, Y.; Xu, L. L.; Nageswararao, C.; Davis, L. D.; Segawa, T.; Dobi, A.; McLeod, D. G.; Srivastava, S. Androgen receptor mutation (T877A) promotes prostate cancer cell growth and cell survival Oncogene 2006, 25 (28) 3905-13
46. Yardy, G. W.; Brewster, S. F. Wnt signalling and prostate cancer Prostate Cancer Prostatic Dis. 2005, 8 (2) 119-26
47. Brand, T. C.; Bermejo, C.; Canby-Hagino, E.; Troyer, D. A.; Baillargeon, J.; Thompson, I. M.; Leach, R. J.; Naylor, S. L. Association of polymorphisms in TGFB1 and prostate cancer prognosis J. Urol. 2008, 179 (2) 754-8
48. Takayama, K.; Tsutsumi, S.; Suzuki, T.; Horie-Inoue, K.; Ikeda, K.; Kaneshiro, K.; Fujimura, T.; Kumagai, J.; Urano, T.; Sakaki, Y.; Shirahige, K.; Sasano, H.; Takahashi, S.; Kitamura, T.; Ouchi, Y.; Aburatani, H.; Inoue, S. Amyloid precursor protein Is a primary androgen target gene that promotes prostate cancer growth Cancer Res. 2009, 69 (1) 137-42
49. Chetcuti, A.; Margan, S. H.; Russell, P.; Mann, S.; Millar, D. S.; Clark, S. J.; Rogers, J.; Handelsman, D. J.; Dong, Q. Loss of annexin II heavy and light chains in prostate cancer and its precursors Cancer Res. 2001, 61 (17) 6331-4
50. Gross, M.; Top, I.; Laux, I.; Katz, J.; Curran, J.; Tindell, C.; Agus, D. β-2-microglobulin Is an androgen-regulated secreted protein elevated in derum of patients with advanced prostate cancer Clin. Cancer Res. 2007, 13 (7) 1979-86
51. Bianchi-Frias, D.; Pritchard, C.; Mecham, B. H.; Coleman, I. M.; Nelson, P. S. Genetic background influences murine prostate gene expression: implications for cancer phenotypes Genome Biol. 2007, 8 (6) R117.1-R.15
52. Sivanandam, A.; Murthy, S.; Chinnakannu, K.; Bai, V. U.; Kim, S.-H.; Barrack, E. R.; Menon, M.; Reddy, G. P.-V. Calmodulin protects androgen receptor from calpain-mediated breakdown in prostate cancer cells J. Cell. Physiol. 2011, 226 (7) 1889-96
53. Wang, J. C.; Bégin, L. R.; Bérubé, N. G.; Chevalier, S.; Aprikian, A. G.; Gourdeau, H.; Chevrette, M. Down-regulation of CD9 expression during prostate carcinoma progression is associated with CD9 mRNA modifications Clin. Cancer Res. 2007, 13 (8) 2354-61
54. Rauhala, H. E.; Porkka, K. P.; Saramäki, O. R.; Tammela, T. L. J.; Visakorpi, T. Clusterin is epigenetically regulated in prostate cancer Int. J. Cancer 2008, 123 (7) 1601-9
55. Miyake, H.; Hara, I.; Gleave, M. E. Antisense oligodeoxynucleotide therapy targeting clusterin gene for prostate cancer: Vancouver experience from discovery to clinic Int. J. Urol. 2005, 12 (9) 785-94
56. Tumminello, F. M.; Flandina, C.; Crescimanno, M.; Leto, G. Circulating cathepsin K and cystatin C in patients with cancer related bone disease: Clinical and therapeutic implications Biomed. Pharmacother. 2008, 62 (2) 130-5
57. Khan, A. P.; Poisson, L. M.; Bhat, V. B.; Fermin, D.; Zhao, R.; Kalyana-Sundaram, S.; Michailidis, G.; Nesvizhskii, A. I.; Omenn, G. S.; Chinnaiyan, A. M.; Sreekumar, A. Quantitative proteomic profiling of prostate cancer reveals a role for miR-128 in prostate cancer Mol. Cell. Proteomics 2010, 9 (2) 298-312
58. Santamaria, I.; Velasco, G.; Pendas, A. M.; Fueyo, A.; Lopez-Otin, C.; Cathepsin, Z a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location J. Biol. Chem. 1998, 273 (27) 16816-23
59. Sgambato, A.; De Paola, B.; Migaldi, M.; Di Salvatore, M.; Rettino, A.; Rossi, G.; Faraglia, B.; Boninsegna, A.; Maiorana, A.; Cittadini, A. Dystroglycan expression is reduced during prostate tumorigenesis and is regulated by androgens in prostate cancer cells J. Cell. Physiol. 2007, 213 (2) 528-39
60. Bedolla, R. G.; Wang, Y.; Asuncion, A.; Chamie, K.; Siddiqui, S.; Mudryj, M. M.; Prihoda, T. J.; Siddiqui, J.; Chinnaiyan, A. M.; Mehra, R.; de Vere White, R. W.; Ghosh, P. M. Nuclear versus cytoplasmic localization of filamin A in prostate cancer: immunohistochemical correlation with metastases Clin. Cancer Res. 2009, 15 (3) 788-96
61. Trojan, L.; Schaaf, A.; Steidler, A.; Haak, M.; Thalmann, G.; Knoll, T.; Gretz, N.; Alken, P.; Michel, M. S. Identification of metastasis-associated genes in prostate cancer by genetic profiling of human prostate cancer cell lines Anticancer Res. 2005, 25 (1A) 183-91
62. Rondinelli, R. H.; Epner, D. E.; Tricoli, J. V. Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in late pathological stage human prostate cancer Prostate Cancer Prostatic Dis. 1997, 1 (2) 66-72
63. Nakayama, M.; Gonzalgo, M. L.; Yegnasubramanian, S.; Lin, X.; Marzo, A. M. D.; Nelson, W. G. GSTP1 CpG island hypermethylation as a molecular biomarker for prostate cancer J. Cell. Biochem. 2004, 91 (3) 540-52
64. Karan, D.; Kelly, D. L.; Rizzino, A.; Lin, M.; Batra, S. K. Expression profile of differentially-regulated genes during progression of androgen-independent growth in human prostate cancer cells Carcinogenesis 2002, 23 (6) 967-76
65. Ashida, S.; Nakagawa, H.; Katagiri, T.; Furihata, M.; Iiizumi, M.; Anazawa, Y.; Tsunoda, T.; Takata, R.; Kasahara, K.; Miki, T.; Fujioka, T.; Shuin, T.; Nakamura, Y. Molecular features of the transition from prostatic intraepithelial neoplasia (PIN) to prostate cancer: genome-wide gene-expression profiles of prostate cancers and PINs Cancer Res. 2004, 64 (17) 5963-72
66. Eklöv, S.; Funa, K.; Nordgren, H.; Olofsson, A.; Kanzaki, T.; Miyazono, K.; Nilsson, S. Lack of the latent transforming growth factor β binding protein in malignant, but not benign prostatic tissue Cancer Res. 1993, 53 (13) 3193-7
67. Latil, A.; Bièche, I.; Pesche, S.; Valéri, A.; Fournier, G.; Cussenot, O.; Lidereau, R. VEGF overexpression in clinically localized prostate tumors and neuropilin-1 overexpression in metastatic forms Int. J. Cancer 2000, 89 (2) 167-71
68. Romanuik, T. L.; Ueda, T.; Le, N.; Haile, S.; Yong, T. M. K.; Thomson, T.; Vessella, R. L.; Sadar, M. D. Novel biomarkers for prostate cancer including noncoding transcripts Am. J. Pathol. 2009, 175 (6) 2264-76
69. Chhipa, R. R.; Lee, K.-S.; Onate, S.; Wu, Y.; Ip, C. Prx1 enhances androgen receptor function in prostate cancer cells by increasing receptor affinity to dihydrotestosterone Mol. Cancer Res. 2009, 7 (9) 1543-52
70. Valdman, A.; Hǎggarth, L.; Cheng, L.; Lopez-Beltran, A.; Montironi, R.; Ekman, P.; Egevad, L. Expression of redox pathway enzymes in human prostatic tissue Anal. Quant. Cytol. Histol. 2009, 31 (6) 367-74
71. Chu, T. M.; Wang, M. C.; Kuciel, R.; Valenzuela, L.; Murphy, G. P. Enzyme markers in human prostatic carcinoma Cancer Treat Rep. 1977, 61 (2) 193-200
72. Rehman, I.; Azzouzi, A. R.; Cross, S. S.; Deloulme, J. C.; Catto, J. W. F.; Wylde, N.; Larre, S.; Champigneuille, J.; Hamdy, F. C. Dysregulated expression of S100A11 (calgizzarin) in prostate cancer and precursor lesions Hum. Pathol. 2004, 35 (11) 1385-91
73. Bostwick, D. G.; Alexander, E. E.; Singh, R.; Shan, A.; Qian, J.; Santella, R. M.; Oberley, L. W.; Yan, T.; Zhong, W.; Jiang, X.; Oberley, T. D. Antioxidant enzyme expression and reactive oxygen species damage in prostatic intraepithelial neoplasia and cancer Cancer 2000, 89 (1) 123-34
74. Wlazlinski, A.; Engers, R.; Hoffmann, M. J.; Hader, C.; Jung, V.; Müller, M.; Schulz, W. A. Downregulation of several fibulin genes in prostate cancer Prostate 2007, 67 (16) 1770-80
75. Pulukuri, S. M.; Patibandla, S.; Patel, J.; Estes, N.; Rao, J. S. Epigenetic inactivation of the tissue inhibitor of metalloproteinase-2 (TIMP-2) gene in human prostate tumors Oncogene 2007, 26 (36) 5229-37
76. Qian, X. L.; Shi, Q. G.; Pang, B.; Wu, R. Q.; Yu, L.; Li, S. H.; Wang, H. T.; Zhou, J. G. Identification and expression of two new secretory proteins associated with prostate cancer Yichuan 2010, 32, 235-41
77. Wu, M.; Bai, X.; Xu, G.; Wei, J.; Zhu, T.; Zhang, Y.; Li, Q.; Liu, P.; Song, A.; Zhao, L.; Gang, C.; Han, Z.; Wang, S.; Zhou, J.; Lu, Y.; Ma, D. Proteome analysis of human androgen-independent prostate cancer cell lines: Variable metastatic potentials correlated with vimentin expression Proteomics 2007, 7 (12) 1973-83