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Volumn 1, Issue 6, 2007, Pages 2650-2660

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGININE; PROLINE; PROTEIN;

EID: 34247396011     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.427     Document Type: Article
Times cited : (733)

References (43)
  • 1
    • 0037435030 scopus 로고    scopus 로고
    • Mass spectrometry-based proteomics
    • Aebersold, R. & Mann, M. Mass spectrometry-based proteomics. Nature 422, q198-207 (2003).
    • (2003) Nature , vol.422
    • Aebersold, R.1    Mann, M.2
  • 2
    • 33644524918 scopus 로고    scopus 로고
    • Mass spectrometry-based proteomics turns quantitative
    • Ong, S.E. & Mann, M. Mass spectrometry-based proteomics turns quantitative. Nat. Chem. Biol. 1, 252-262 (2005).
    • (2005) Nat. Chem. Biol , vol.1 , pp. 252-262
    • Ong, S.E.1    Mann, M.2
  • 3
    • 0036583926 scopus 로고    scopus 로고
    • Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics
    • Ong, S.E. et al. Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 1, 376-386 (2002).
    • (2002) Mol. Cell. Proteomics , vol.1 , pp. 376-386
    • Ong, S.E.1
  • 4
    • 0036665484 scopus 로고    scopus 로고
    • Quantitative analysis of the yeast proteome by incorporation of isotopically labeled leucine
    • Jiang, H. & English, A.M. Quantitative analysis of the yeast proteome by incorporation of isotopically labeled leucine. J. Proteome Res. 1, 345-350 (2002).
    • (2002) J. Proteome Res , vol.1 , pp. 345-350
    • Jiang, H.1    English, A.M.2
  • 5
    • 0036428393 scopus 로고    scopus 로고
    • Amino acid residue specific stable isotope labeling for quantitative proteomics
    • Zhu, H., Pan, S., Gu, S., Bradbury, E.M. & Chen, X. Amino acid residue specific stable isotope labeling for quantitative proteomics. Rapid Commun. Mass Spectrom. 16, 2115-2123 (2002).
    • (2002) Rapid Commun. Mass Spectrom , vol.16 , pp. 2115-2123
    • Zhu, H.1    Pan, S.2    Gu, S.3    Bradbury, E.M.4    Chen, X.5
  • 6
    • 0012351964 scopus 로고    scopus 로고
    • 13C-substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC)
    • 13C-substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC). J. Proteome Res. 2, 173-181 (2003).
    • (2003) J. Proteome Res , vol.2 , pp. 173-181
    • Ong, S.E.1    Kratchmarova, I.2    Mann, M.3
  • 7
    • 0033535961 scopus 로고    scopus 로고
    • Accurate quantitation of protein expression and site-specific phosphorylation
    • Oda, Y., Huang, K., Cross, F.R., Cowburn, D. & Chait, B.T. Accurate quantitation of protein expression and site-specific phosphorylation. Proc. Natl. Acad. Sci. USA 96, 6591-6596 (1999).
    • (1999) Proc. Natl. Acad. Sci. USA , vol.96 , pp. 6591-6596
    • Oda, Y.1    Huang, K.2    Cross, F.R.3    Cowburn, D.4    Chait, B.T.5
  • 8
    • 33845329203 scopus 로고    scopus 로고
    • Functional and quantitative proteomics using SILAC
    • Mann, M. Functional and quantitative proteomics using SILAC. Nat. Rev. Mol. Cell. Biol. 7, 952-958 (2006).
    • (2006) Nat. Rev. Mol. Cell. Biol , vol.7 , pp. 952-958
    • Mann, M.1
  • 9
    • 15944377809 scopus 로고    scopus 로고
    • Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway
    • Gruhler, A. et al. Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway. Mol. Cell. Proteomics 4, 310-327 (2005).
    • (2005) Mol. Cell. Proteomics , vol.4 , pp. 310-327
    • Gruhler, A.1
  • 10
    • 22744447508 scopus 로고    scopus 로고
    • Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli
    • Kerner, M.J. et al. Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli. Cell 122, 209-220 (2005).
    • (2005) Cell , vol.122 , pp. 209-220
    • Kerner, M.J.1
  • 11
    • 3142671580 scopus 로고    scopus 로고
    • Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition
    • Nirmalan, N., Sims, P.F. & Hyde, J.E. Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition. Mol. Microbiol. 52 1187-1199 (2004).
    • (2004) Mol. Microbiol , vol.52 , pp. 1187-1199
    • Nirmalan, N.1    Sims, P.F.2    Hyde, J.E.3
  • 12
    • 28644448658 scopus 로고    scopus 로고
    • Stable isotope labeling of Arabidopsis thaliana cells and quantitative proteomics by mass spectrometry
    • Gruhler, A., Schulze, W.X., Matthiesen, R., Mann, M. & Jensen, O.N. Stable isotope labeling of Arabidopsis thaliana cells and quantitative proteomics by mass spectrometry. Mol. Cell. Proteomics 4, 1697-1709 (2005).
    • (2005) Mol. Cell. Proteomics , vol.4 , pp. 1697-1709
    • Gruhler, A.1    Schulze, W.X.2    Matthiesen, R.3    Mann, M.4    Jensen, O.N.5
  • 13
    • 1642314524 scopus 로고    scopus 로고
    • A novel proteomic screen for peptide-protein interactions
    • Schulze, W.X. & Mann, M. A novel proteomic screen for peptide-protein interactions. J. Biol. Chem. 279, 10756-10764 (2004).
    • (2004) J. Biol. Chem , vol.279 , pp. 10756-10764
    • Schulze, W.X.1    Mann, M.2
  • 14
    • 2542477014 scopus 로고    scopus 로고
    • RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers
    • de Hoog, C.L., Foster, L.J. & Mann, M. RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers. Cell 117, 649-662 (2004).
    • (2004) Cell , vol.117 , pp. 649-662
    • de Hoog, C.L.1    Foster, L.J.2    Mann, M.3
  • 15
    • 0037337310 scopus 로고    scopus 로고
    • A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling
    • Blagoev, B. et al. A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling. Nat. Biotechnol. 21, 315-318 (2003).
    • (2003) Nat. Biotechnol , vol.21 , pp. 315-318
    • Blagoev, B.1
  • 16
    • 11944274520 scopus 로고    scopus 로고
    • Nucleolar proteome dynamics
    • Andersen, J.S. et al. Nucleolar proteome dynamics. Nature 433, 77-83 (2005).
    • (2005) Nature , vol.433 , pp. 77-83
    • Andersen, J.S.1
  • 17
    • 4444371652 scopus 로고    scopus 로고
    • Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics
    • Blagoev, B., Ong, S.E., Kratchmarova, I. & Mann, M. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat. Biotechnol. 22, 1139-1145 (2004).
    • (2004) Nat. Biotechnol , vol.22 , pp. 1139-1145
    • Blagoev, B.1    Ong, S.E.2    Kratchmarova, I.3    Mann, M.4
  • 18
    • 22844436250 scopus 로고    scopus 로고
    • Quantitative mouse brain proteomics using culture-derived isotope tags as internal standards
    • Ishihama, Y. et al. Quantitative mouse brain proteomics using culture-derived isotope tags as internal standards. Nat. Biotechnol. 23, 617-621 (2005).
    • (2005) Nat. Biotechnol , vol.23 , pp. 617-621
    • Ishihama, Y.1
  • 19
    • 34548819163 scopus 로고    scopus 로고
    • In gel digestion for mass spectrometric characterization of proteins and proteomes
    • in the press
    • Shevchenko, A., Tomas, H., Havlis, J., Olsen, J.V. & Mann, M. In gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protocols (in the press).
    • Nat. Protocols
    • Shevchenko, A.1    Tomas, H.2    Havlis, J.3    Olsen, J.V.4    Mann, M.5
  • 20
    • 31644434206 scopus 로고    scopus 로고
    • Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach
    • Gronborg, M. et al. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol. Cell. Proteomics 5, 157-171 (2006).
    • (2006) Mol. Cell. Proteomics , vol.5 , pp. 157-171
    • Gronborg, M.1
  • 21
    • 33750141410 scopus 로고    scopus 로고
    • Secreted proteome profiling in human RPE cell cultures derived from donors with age related macular degeneration and age matched healthy donors
    • An, E. et al. Secreted proteome profiling in human RPE cell cultures derived from donors with age related macular degeneration and age matched healthy donors. J. Proteome Res. 5, 2599-2610 (2006).
    • (2006) J. Proteome Res , vol.5 , pp. 2599-2610
    • An, E.1
  • 22
    • 19944432197 scopus 로고    scopus 로고
    • Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents
    • Ross, P.L. et al. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol. Cell. Proteomics (2004).
    • (2004) Mol. Cell. Proteomics
    • Ross, P.L.1
  • 23
    • 4444335470 scopus 로고    scopus 로고
    • The ABC's (and XYZ's) of peptide sequencing
    • Steen, H. & Mann, M. The ABC's (and XYZ's) of peptide sequencing. Nat. Rev. Mol. Cell Biol. 5, 699-711 (2004).
    • (2004) Nat. Rev. Mol. Cell Biol , vol.5 , pp. 699-711
    • Steen, H.1    Mann, M.2
  • 24
    • 30144443840 scopus 로고    scopus 로고
    • Peptide sequence analysis
    • Medzihradszky, K.F. Peptide sequence analysis. Methods Enzymol. 402, 209-244 (2005).
    • (2005) Methods Enzymol , vol.402 , pp. 209-244
    • Medzihradszky, K.F.1
  • 25
    • 4043141477 scopus 로고    scopus 로고
    • Quantitative cancer proteomics: Stable isotope labeling with amino acids in cell culture (SILAC) as a tool for prostate cancer research
    • Everley, P.A., Krijgsveld, J., Zetter, B.R. & Gygi, S.P. Quantitative cancer proteomics: Stable isotope labeling with amino acids in cell culture (SILAC) as a tool for prostate cancer research. Mol. Cell. Proteomics 3, 729-735 (2004).
    • (2004) Mol. Cell. Proteomics , vol.3 , pp. 729-735
    • Everley, P.A.1    Krijgsveld, J.2    Zetter, B.R.3    Gygi, S.P.4
  • 26
    • 18744375196 scopus 로고    scopus 로고
    • Identifying and quantifying in vivo methylation sites by heavy methyl SILAC
    • Ong, S.E., Mittler, G. & Mann, M. Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat. Methods 1, 119-126 (2004).
    • (2004) Nat. Methods , vol.1 , pp. 119-126
    • Ong, S.E.1    Mittler, G.2    Mann, M.3
  • 27
    • 3042815334 scopus 로고    scopus 로고
    • Trypsin cleaves exclusively C-terminal to arginine and lysine residues
    • Olsen, J.V., Ong, S.E. & Mann, M. Trypsin cleaves exclusively C-terminal to arginine and lysine residues. Mol. Cell. Proteomics 3, 608-614 (2004).
    • (2004) Mol. Cell. Proteomics , vol.3 , pp. 608-614
    • Olsen, J.V.1    Ong, S.E.2    Mann, M.3
  • 28
    • 0242569349 scopus 로고    scopus 로고
    • A proteomic approach for quantitation of phosphorylation using stable isotope labeling in cell culture
    • Ibarrola, N., Kalume, D.E., Gronborg, M., Iwahori, A. & Pandey, A. A proteomic approach for quantitation of phosphorylation using stable isotope labeling in cell culture. Anal. Chem. 75, 6043-6049 (2003).
    • (2003) Anal. Chem , vol.75 , pp. 6043-6049
    • Ibarrola, N.1    Kalume, D.E.2    Gronborg, M.3    Iwahori, A.4    Pandey, A.5
  • 29
    • 0037338365 scopus 로고    scopus 로고
    • Proteomic analysis of post-translational modifications
    • Mann, M. & Jensen, O.N. Proteomic analysis of post-translational modifications. Nat. Biotechnol. 21, 255-261 (2003).
    • (2003) Nat. Biotechnol , vol.21 , pp. 255-261
    • Mann, M.1    Jensen, O.N.2
  • 30
    • 33747592823 scopus 로고    scopus 로고
    • Graded regulation of the Kv2.1 potassium channel by variable phosphorylation
    • Park, K.S., Mohapatra, D.P., Misonou, H. & Trimmer, J.S. Graded regulation of the Kv2.1 potassium channel by variable phosphorylation. Science 313, 976-979 (2006).
    • (2006) Science , vol.313 , pp. 976-979
    • Park, K.S.1    Mohapatra, D.P.2    Misonou, H.3    Trimmer, J.S.4
  • 31
    • 20344363684 scopus 로고    scopus 로고
    • Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation
    • Kratchmarova, I., Blagoev, B., Haack-Sorensen, M., Kassem, M. & Mann, M. Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation. Science 308, 1472-1477 (2005).
    • (2005) Science , vol.308 , pp. 1472-1477
    • Kratchmarova, I.1    Blagoev, B.2    Haack-Sorensen, M.3    Kassem, M.4    Mann, M.5
  • 32
    • 0036692065 scopus 로고    scopus 로고
    • Dynamics of protein turnover, a missing dimension in proteomics
    • Pratt, J.M. et al. Dynamics of protein turnover, a missing dimension in proteomics. Mol. Cell. Proteomics 1, 579-591 (2002).
    • (2002) Mol. Cell. Proteomics , vol.1 , pp. 579-591
    • Pratt, J.M.1
  • 33
    • 29144480597 scopus 로고    scopus 로고
    • Analysis of protein expression during oxidative stress in breast epithelial cells using a stable isotope labeled proteome internal standard
    • Yan, Y., Weaver, V.M. & Blair, I.A. Analysis of protein expression during oxidative stress in breast epithelial cells using a stable isotope labeled proteome internal standard. J. Proteome Res. 4 2007-2014 (2005).
    • (2005) J. Proteome Res , vol.4 , pp. 2007-2014
    • Yan, Y.1    Weaver, V.M.2    Blair, I.A.3
  • 35
    • 0036523809 scopus 로고    scopus 로고
    • Minimizing resolution of isotopically coded peptides in comparative proteomics
    • Zhang, R. & Regnier, F.E. Minimizing resolution of isotopically coded peptides in comparative proteomics. J. Proteome Res. 1, 139-147 (2002).
    • (2002) J. Proteome Res , vol.1 , pp. 139-147
    • Zhang, R.1    Regnier, F.E.2
  • 36
    • 0037270746 scopus 로고    scopus 로고
    • Precise peptide sequencing and protein quantification in the human proteome through in vivo lysine-specific mass tagging
    • Gu, S., Pan, S., Bradbury, E.M. & Chen, X. Precise peptide sequencing and protein quantification in the human proteome through in vivo lysine-specific mass tagging. J. Am. Soc. Mass Spectrom. 14, 1-7 (2003).
    • (2003) J. Am. Soc. Mass Spectrom , vol.14 , pp. 1-7
    • Gu, S.1    Pan, S.2    Bradbury, E.M.3    Chen, X.4
  • 37
    • 0033822017 scopus 로고    scopus 로고
    • Single amino acid (arginine) deprivation: Rapid and selective death of cultured transformed and malignant cells
    • Scott, L., Lamb, J., Smith, S. & Wheatley, D.N. Single amino acid (arginine) deprivation: Rapid and selective death of cultured transformed and malignant cells. Br. J. Cancer 83, 800-810 (2000).
    • (2000) Br. J. Cancer , vol.83 , pp. 800-810
    • Scott, L.1    Lamb, J.2    Smith, S.3    Wheatley, D.N.4
  • 38
    • 0033926022 scopus 로고    scopus 로고
    • Single amino acid (arginine) restriction: Growth and death of cultured HeLa and human diploid fibroblasts
    • Wheatley, D.N., Scott, L., Lamb, J. & Smith, S. Single amino acid (arginine) restriction: Growth and death of cultured HeLa and human diploid fibroblasts. Cell. Physiol. Biochem. 10, 37-55 (2000).
    • (2000) Cell. Physiol. Biochem , vol.10 , pp. 37-55
    • Wheatley, D.N.1    Scott, L.2    Lamb, J.3    Smith, S.4
  • 40
    • 7044229935 scopus 로고    scopus 로고
    • Evaluation of metabolic labeling for comparative proteomics in breast cancer cells
    • Gehrmann, M.L., Hathout, Y. & Fenselau, C. Evaluation of metabolic labeling for comparative proteomics in breast cancer cells. J. Proteome Res. 3, 1063-1068 (2004).
    • (2004) J. Proteome Res , vol.3 , pp. 1063-1068
    • Gehrmann, M.L.1    Hathout, Y.2    Fenselau, C.3
  • 41
    • 0037317228 scopus 로고    scopus 로고
    • Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics
    • Rappsilber, J., Ishihama, Y. & Mann, M. Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal. Chem. 75, 663-670 (2003).
    • (2003) Anal. Chem , vol.75 , pp. 663-670
    • Rappsilber, J.1    Ishihama, Y.2    Mann, M.3
  • 42
    • 3042665732 scopus 로고    scopus 로고
    • Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis
    • Candiano, G. et al. Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 1327-1333 (2004).
    • (2004) Electrophoresis , vol.25 , pp. 1327-1333
    • Candiano, G.1
  • 43
    • 3042735127 scopus 로고    scopus 로고
    • The need for guidelines in publication of peptide and protein identification data: Working Group on Publication Guidelines for Peptide and Protein Identification Data
    • Carr, S. et al. The need for guidelines in publication of peptide and protein identification data: Working Group on Publication Guidelines for Peptide and Protein Identification Data. Mol. Cell. Proteomics 3, 531-533 (2004).
    • (2004) Mol. Cell. Proteomics , vol.3 , pp. 531-533
    • Carr, S.1


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