Identification and characterization of an immunogenic hybrid epitope formed by both HIV gp120 and human CD4 proteins

Journal of Virology

Volumn 85, Issue 24, 2011, Pages 13097-13104

  Lewis, George K ^a   Fouts, Timothy R ^b   Ibrahim, Sani ^c   Taylor, Brian M ^a   Salkar, Rachita ^a   Guan, Yongjun ^a   Kamin Lewis, Roberta ^a   DeVico, Anthony L ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b Profectus BioSciences Inc   (United States)

^c AHMADU BELLO UNIVERSITY   (Nigeria)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; EPITOPE; GLYCOPROTEIN GP 120; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 19E; MONOCLONAL ANTIBODY 21C; UNCLASSIFIED DRUG;

ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ANTIGEN DETECTION; ANTIGEN STRUCTURE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; GENE MUTATION; HUMAN; HUMAN CELL; HYBRID; IMMUNOCHEMISTRY; IMMUNOGENETICS; IMMUNOREACTIVITY; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN INTERACTION; VIRUS CELL INTERACTION; VIRUS EXAMINATION;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIGENS, CD4; EPITOPES; HIV ANTIBODIES; HIV ENVELOPE PROTEIN GP120; HUMANS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; PROTEIN BINDING;

EID: 84855846601     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.05072-11     Document Type: Article

References (57)

1. Ashkenazi, A., et al. 1990. Mapping the CD4 binding site for human immunodeficiency virus by alanine-scanning mutagenesis. Proc. Natl. Acad. Sci. U. S. A. 87:7150-7154.
2. Brodsky, M. H., M. Warton, R. M. Myers, and D. R. Littman. 1990. Analysis of the site in CD4 that binds to the HIV envelope glycoprotein. J. Immunol. 144:3078-3086.
3. Burton, D. R., et al. 2004. HIV vaccine design and the neutralizing antibody problem. Nat. Immunol. 5:233-236.
4. Burton, D. R., R. L. Stanfield, and I. A. Wilson. 2005. Antibody vs. HIV in a clash of evolutionary titans. Proc. Natl. Acad. Sci. U. S. A. 102:14943-14948.
5. Chen, B., et al. 2005. Structure of an unliganded simian immunodeficiency virus gp120 core. Nature 433:834-841.
6. Cocchi, F., et al. 1996. The V3 domain of the HIV-1 gp120 envelope glycoprotein is critical for chemokine-mediated blockade of infection. Nat. Med. 2:1244-1247.
7. Crooks, E. T., et al. 2005. Characterizing anti-HIV monoclonal antibodies and immune sera by defining the mechanism of neutralization. Hum. Antibodies 14:101-113.
8. Dalgleish, A. G., et al. 1984. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 312:763-767.
9. Decker, J. M., et al. 2005. Antigenic conservation and immunogenicity of the HIV coreceptor binding site. J. Exp. Med. 201:1407-1419.
10. DeVico, A. L. 2007. CD4-induced epitopes in the HIV envelope glycoprotein, gp120. Curr. HIV Res. 5:561-571.
11. Diskin, R., P. M. Marcovecchio, and P. J. Bjorkman. 2010. Structure of a clade C HIV-1 gp120 bound to CD4 and CD4-induced antibody reveals anti-CD4 polyreactivity. Nat. Struct. Mol. Biol. 17:608-613.
12. Douagi, I., et al. 2010. Influence of novel CD4 binding-defective HIV-1 envelope glycoprotein immunogens on neutralizing antibody and T-cell responses in non-human primates. J. Virol. 84:1683-1695.
13. Ferrari, G., et al. 2011. An HIV-1 gp120 envelope human monoclonal antibody that recognizes a C1 conformational epitope mediates potent antibody-dependent cellular cytotoxicity (ADCC) activity and defines a common ADCC epitope in human HIV-1 serum. J. Virol. 85:7029-7036.
14. Finnegan, C. M., W. Berg, G. K. Lewis, and A. L. DeVico. 2001. Antigenic properties of the human immunodeficiency virus envelope during cell-cell fusion. J. Virol. 75:11096-11105.
15. Forsell, M. N., et al. 2008. B cell recognition of the conserved HIV-1 coreceptor binding site is altered by endogenous primate CD4. PLoS Pathog. 4:e1000171.
16. Fouts, T., et al. 2002. Crosslinked HIV-1 envelope-CD4 receptor complexes elicit broadly cross-reactive neutralizing antibodies in rhesus macaques. Proc. Natl. Acad. Sci. U. S. A. 99:11842-11847.
17. Fouts, T. R., et al. 2000. Expression and characterization of a single-chain polypeptide analogue of the human immunodeficiency virus type 1 gp120-CD4 receptor complex. J. Virol. 74:11427-11436.
18. Gilljam, G. 1993. Envelope glycoproteins of HIV-1, HIV-2, and SIV purified with Galanthus nivalis agglutinin induce strong immune responses. AIDS Res. Hum. Retroviruses 9:431-438.
19. Gnanakaran, S., et al. 2010. Genetic signatures in the envelope glycoproteins of HIV-1 that associate with broadly neutralizing antibodies. PLoS Comput. Biol. 6:e1000955.
20. Gray, E. S., et al. 2007. Neutralizing antibody responses in acute human immunodeficiency virus type 1 subtype C infection. J. Virol. 81:6187-6196.
21. Guan, Y., et al. 2009. Discordant memory B cell and circulating anti-Env antibody responses in HIV-1 infection. Proc. Natl. Acad. Sci. U. S. A. 106:3952-3957.
22. Hart, T. K., et al. 1991. Binding of soluble CD4 proteins to human immunodeficiency virus type 1 and infected cells induces release of envelope glycoprotein gp120. Proc. Natl. Acad. Sci. U. S. A. 88:2189-2193.
23. Huang, C. C., et al. 2005. Structure of a V3-containing HIV-1 gp120 core. Science 310:1025-1028.
24. Huang, C. C., et al. 2004. Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120. Proc. Natl. Acad. Sci. U. S. A. 101:2706-2711.
25. Kirchherr, J. L., et al. 2011. Identification of amino acid substitutions associated with neutralization phenotype in the human immunodeficiency virus type-1 subtype C gp120. Virology 409:163-174.
26. Kwong, P. D., et al. 2000. Structures of HIV-1 gp120 envelope glycoproteins from laboratory-adapted and primary isolates. Structure 8:1329-1339.
27. Kwong, P. D., et al. 1998. Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. Nature 393:648-659.
28. Kwong, P. D., R. Wyatt, Q. J. Sattentau, J. Sodroski, and W. A. Hendrickson. 2000. Oligomeric modeling and electrostatic analysis of the gp120 envelope glycoprotein of human immunodeficiency virus. J. Virol. 74:1961-1972.
29. Labrijn, A. F., et al. 2003. Access of antibody molecules to the conserved coreceptor binding site on glycoprotein gp120 is sterically restricted on primary human immunodeficiency virus type 1. J. Virol. 77:10557-10565.
30. Lenert, P., D. Kroon, H. Spiegelberg, E. S. Golub, and M. Zanetti. 1990. Human CD4 binds immunoglobulins. Science 248:1639-1643.
31. Li, Y., et al. 2009. Analysis of neutralization specificities in polyclonal sera derived from human immunodeficiency virus type 1-infected individuals. J. Virol. 83:1045-1059.
32. McMichael, A. J., P. Borrow, G. D. Tomaras, N. Goonetilleke, and B. F. Haynes. 2010. The immune response during acute HIV-1 infection: clues for vaccine development. Nat. Rev. Immunol. 10:11-23.
33. Moebius, U., L. K. Clayton, S. Abraham, S. C. Harrison, and E. L. Reinherz. 1992. The human immunodeficiency virus gp120 binding site on CD4: delineation by quantitative equilibrium and kinetic binding studies of mutants in conjunction with a high-resolution CD4 atomic structure. J. Exp. Med. 176:507-517.
34. Moore, J. P., and J. Sodroski. 1996. Antibody cross-competition analysis of the human immunodeficiency virus type 1 gp120 exterior envelope glycoprotein. J. Virol. 70:1863-1872.
35. Moore, J. P., R. L. Willey, G. K. Lewis, J. Robinson, and J. Sodroski. 1994. Immunological evidence for interactions between the first, second, and fifth conserved domains of the gp120 surface glycoprotein of human immunodeficiency virus type 1. J. Virol. 68:6836-6847.
36. Pal, R., A. DeVico, S. Rittenhouse, and M. G. Sarngadharan. 1993. Conformational perturbation of the envelope glycoprotein gp120 of human immunodeficiency virus type 1 by soluble CD4 and the lectin succinyl Con A. Virology 194:833-837.
37. Poignard, P., E. O. Saphire, P. W. Parren, and D. R. Burton. 2001. Gp120: biologic aspects of structural features. Annu. Rev. Immunol. 19:253-274.
38. Robinson, J. E., H. Yoshiyama, D. Holton, S. Elliott, and D. D. Ho. 1992. Distinct antigenic sites on HIV gp120 identified by a panel of human monoclonal antibodies. J. Cell. Biochem. 50(Suppl. 16E):71.
39. Sattentau, Q. J., J. P. Moore, F. Vignaux, F. Traincard, and P. Poignard. 1993. Conformational changes induced in the envelope glycoproteins of the human and simian immunodeficiency viruses by soluble receptor binding. J. Virol. 67:7383-7393.
40. Scheid, J. F., et al. 2009. Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals. Nature 458:636-640.
41. Schneidman-Duhovny, D., Y. Inbar, R. Nussinov, and H. J. Wolfson. 2005. PatchDock and SymmDock: servers for rigid and symmetric docking. Nucleic Acids Res. 33:W363-W367.
42. Schneidman-Duhovny, D., et al. 2003. Taking geometry to its edge: fast unbound rigid (and hinge-bent) docking. Proteins 52:107-112.
43. Steckbeck, J. D., et al. 2005. Kinetic rates of antibody binding correlate with neutralization sensitivity of variant simian immunodeficiency virus strains. J. Virol. 79:12311-12320.
44. Sullivan, N., et al. 1998. CD4-induced conformational changes in the human immunodeficiency virus type 1 gp120 glycoprotein: consequences for virus entry and neutralization. J. Virol. 72:4694-4703.
45. Taylor, B. M., et al. 2008. An alteration of HIV gp41 leading to reduced CCR5 dependence and CD4 independence. J. Virol. 82:5460-5471.
46. Thali, M., et al. 1993. Characterization of conserved human immunodeficiency virus type 1 gp120 neutralization epitopes exposed upon gp120-CD4 binding. J. Virol. 67:3978-3986.
47. Tomaras, G. D., et al. 2008. Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia. J. Virol. 82:12449-12463.
48. Trkola, A., et al. 1996. CD4-dependent, antibody-sensitive interactions between HIV-1 and its co-receptor CCR-5. Nature 384:184-186.
49. Trkola, A., et al. 1995. Cross-clade neutralization of primary isolates of human immunodeficiency virus type 1 by human monoclonal antibodies and tetrameric CD4-IgG. J. Virol. 69:6609-6617.
50. Trkola, A., et al. 1996. Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70:1100-1108.
51. Vita, C., et al. 1999. Rational engineering of a miniprotein that reproduces the core of the CD4 site interacting with HIV-1 envelope glycoprotein. Proc. Natl. Acad. Sci. U. S. A. 96:13091-13096.
52. Whitelegg, N., and A. R. Rees. 2004. Antibody variable regions: toward a unified modeling method. Methods Mol. Biol. 248:51-91.
53. Whitelegg, N. R., and A. R. Rees. 2000. WAM: an improved algorithm for modelling antibodies on the WEB. Protein Eng. 13:819-824.
54. Wu, L., et al. 1996. CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5. Nature 384:179-183.
55. Wyatt, R., et al. 1998. The antigenic structure of the HIV gp120 envelope glycoprotein. Nature 393:705-711.
56. Wyatt, R., and J. Sodroski. 1998. The HIV-1 envelope glycoproteins: fusogens, antigens, and immunogens. Science 280:1884-1888.
57. Xiang, S. H., N. Doka, R. K. Choudhary, J. Sodroski, and J. E. Robinson. 2002. Characterization of CD4-induced epitopes on the HIV type 1 gp120 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS Res. Hum. Retroviruses 18:1207-1217.