High translation efficiency is mediated by the encephalomyocarditis virus internal ribosomal entry sites if the natural sequence surrounding the eleventh AUG is retained

Human Gene Therapy

Volumn 13, Issue 7, 2002, Pages 881-887

  Qiao, Jian ^a   Roy, Vincent ^a   Girard, Marie Hélène ^a   Caruso, Manuel ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; GREEN FLUORESCENT PROTEIN; LUCIFERASE; RETROVIRUS VECTOR;

ANIMAL CELL; ARTICLE; CELL TYPE; CONTROLLED STUDY; EMBRYO; ENCEPHALOMYOCARDITIS VIRUS; FEMALE; GENE SEQUENCE; GENE STRUCTURE; GENETIC ANALYSIS; HUMAN; HUMAN CELL; INTERNAL RIBOSOME ENTRY SITE; MOUSE; NONHUMAN; RNA TRANSLATION; SEQUENCE ANALYSIS; TRANSGENE;

ANIMALS; BETA-GALACTOSIDASE; BLOTTING, NORTHERN; CHLORAMPHENICOL O-ACETYLTRANSFERASE; ENCEPHALOMYOCARDITIS VIRUS; FLOW CYTOMETRY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUCIFERASES; LUMINESCENT PROTEINS; MICE; MODELS, GENETIC; PLASMIDS; PROTEIN BIOSYNTHESIS; RETROVIRIDAE; RIBOSOMES; TRANSFECTION; TRANSGENES; TUMOR CELLS, CULTURED;

ANIMALIA; ENCEPHALOMYOCARDITIS VIRUS; RNA VIRUSES; UNIDENTIFIED RETROVIRUS;

EID: 0036257417     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/10430340252899046     Document Type: Article

References (26)

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3. Adenovirus-mediated interleukin-12 gene therapy for metastatic colon carcinoma
4. The sequence context of the initiation codon in the encephalomyocarditis virus leader modulates efficiency of internal translation initiation
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6. Quantitative analysis of gene suppression in integrated retrovirus vectors
7. The internal ribosomal entry site of the encephalomyocarditis virus enables reliable coexpression of two transgenes in human primary T lymphocytes
8. The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos
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10. Cap-independent translation of encephalomyocarditis virus RNA: Structural elements of the internal ribosomal entry site and involvement of a cellular 57-kD RNA-binding protein
11. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes
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14. IRES-dependent second gene expression is significantly lower than cap- dependent first gene expression in a bicistronic vector
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