Gambogenic acid induced mitochondrial-dependent apoptosis and referred to Phospho-Erk1/2 and Phospho-p38 MAPK in human hepatoma HepG2 cells

Environmental Toxicology and Pharmacology

Volumn 33, Issue 2, 2012, Pages 181-190

  Yan, Fenggen ^a,b   Wang, Mei ^a   Li, Jiaming ^a   Cheng, Hui ^a   Su, Jingjing ^a   Wang, Xiaoshan ^a   Wu, Haiyun ^a   Xia, Lunzhu ^a   Li, Xiaoxiang ^c   Chang, Hebron C ^d   Li, Qinglin ^a  

^a ANHUI UNIVERSITY OF CHINESE MEDICINE   (China)

^b MACAU UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Macao)

^c Anhui Newstar Pharmaceutical Development Co Ltd   (China)

^d ASIA UNIVERSITY   (Taiwan)

Author keywords

Apoptosis; Gambogenic acid; HepG2; Mitochondrial oxidative stress; Phospho Erk1 2 MAPK; Phospho p38 MAPK

Indexed keywords

ACRIDINE ORANGE; ANTINEOPLASTIC AGENT; CASPASE 3; CASPASE 9; ETHIDIUM BROMIDE; GAMBOGENIC ACID; LIPOCORTIN 5; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE P38; NATURAL PRODUCT; PROTEIN BAX; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOWN REGULATION; DRUG TARGETING; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; MEMBRANE PERMEABILITY; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAINING; TRANSMISSION ELECTRON MICROSCOPY; UPREGULATION; WESTERN BLOTTING;

EID: 84855235682     PISSN: 13826689     EISSN: 18727077     Source Type: Journal    
DOI: 10.1016/j.etap.2011.12.006     Document Type: Article

References (36)

1. Asano J., Chiba K., Tada M., Yoshii T. Cytotoxic xanthones from Garcinia hanburyi. Phytochemistry 1996, 41:815-820.
2. Baskic D., Popovic S., Ristic P., Arsenijevic N.N. Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biol. Int. 2006, 30:924-932.
3. Calviño E., Manjón J.L., Sancho P., Tejedor M.C., Herráez A., Diez J.C. Ganoderma lucidum induced apoptosis in NB4 human leukemia cells: involvement of Akt and Erk. J. Ethnopharmacol. 2010, 128:71-78.
4. Danial N.N., Korsmeyer S.J. Cell death: critical control points. Cell 2004, 116:205-219.
5. Granado-Serrano A.B., Martin M.A., Bravo L., Goya L., Ramos S. Quercetin induces apoptosis via caspase activation, regulation of Bcl-2, and inhibition of PI-3-kinase/Akt and ERK pathways in a human hepatoma cell line (HepG2). J. Nutr. 2006, 136:2715-2721.
6. Han L.-L., Xie L.-P., Li L.-H., Zhang X.-W., Zhang R.-Q., Wang H.-Z. Reactive oxygen species production and Bax/Bcl-2 regulation in honokiol-induced apoptosis in human hepatocellular carcinoma SMMC-7721 cells. Environ. Toxicol. Pharmacol. 2009, 28:97-103.
7. Handayani T., Sakinah S., Nallappan M., Pihie A.h.l. Regulation of p53-, Bcl-2- and caspase-dependent signaling pathway in xanthorrhizol-induced apoptosis of HepG2 hepatoma cells. Anticancer Res. 2007, 27:965-971.
8. Hashem B.E.S., Rudolph K.L. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology 2007, 132:2557-2576.
9. Hockenbery D.M. Targeting mitochondria for cancer therapy. Environ. Mol. Mutagen. 2010, 51:476-489.
10. Hu W., Kavanagh J.J. Anticancer therapy targeting the apoptotic pathway. Lancet Oncol. 2003, 4:721-729.
11. Huang S.-P., Chen J.-C., Wu C.-C., Chen C.-T., Tang N.-Y., Ho Y.-T., Lo C., Lin J.-P., Chung J.-G., Lin J.-G. Capsaicin-induced apoptosis in human hepatoma HepG2 cells. Anticancer Res. 2009, 29:165-174.
12. Ji Y.B., Gao S.Y., Ji C.F., Zou X. Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein. J. Ethnopharmacol. 2008, 115:194-202.
13. Kim J.-S., He L., Lemasters J.J. Mitochondrial permeability transition: a common pathway to necrosis and apoptosis. Biochem. Biophys. Res. Commun. 2003, 304:463-470.
14. Kroemer G., Galluzzi L., Brenner C. Mitochondrial membrane permeabilization in cell death. Physiol. Rev. 2007, 87:99-163.
15. Li Q., Cheng H., Zhu G., Yang L., Zhou A., Wang X., Fang N., Xia L., Su J., Wang M., Peng D., Xu Q. Gambogenic acid inhibits proliferation of A549 cells through apoptosis-inducing and cell cycle arresting. Biol. Pharm. Bull. 2010, 33:415-420.
16. Liu L., Cao Y., Chen C., Zhang X., McNabola A., Wilkie D., Wilhelm S., Lynch M., Carter C. Sorafenib blocks the RAF/MEK/ERK pathway, inhibits tumor angiogenesis, and induces tumor cell apoptosis in hepatocellular carcinoma model PLC/PRF/5. Cancer Res. 2006, 66:11851-11858.
17. Llovet J.M. Updated treatment approach to hepatocellular carcinoma. J. Gastroenterol. 2005, 40:225-235.
18. Luk J.M., Wang X., Liu P., Wong K.F., Chan K.L., Tong Y., Hui C.K., Lau G.K., Fan S.T. Traditional Chinese herbal medicines for treatment of liver fibrosis and cancer: from laboratory discovery to clinical evaluation. Liver Int 2007, 27:879-890.
19. Maiuri M.C., Zalckvar E., Kimchi A., Kroemer G. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat. Rev. Mol. Cell Biol. 2007, 8:741-752.
20. Mielgo A., Torres V.A., Clair K., Barbero S., Stupack D.G. Paclitaxel promotes a caspase 8-mediated apoptosis through death effector domain association with microtubules. Oncogene 2009, 28:3551-3562.
21. Momeny M., Khorramizadeh M.R., Ghaffari S.H., Yousefi M., Yekaninejad M.S., Esmaeili R., Jahanshiri Z., Nooridaloii M.R. Effects of silibinin on cell growth and invasive properties of a human hepatocellular carcinoma cell line, HepG-2, through inhibition of extracellular signal-regulated kinase 1/2 phosphorylation. Eur. J. Pharmacol. 2008, 591:13-20.
22. Motomura M., Kwon K.M., Suh S.-J., Lee Y.-C., Kim Y.-K., Lee I.-S., Kim M.-S., Kwon D.Y., Suzuki I., Kim C.-H. Propolis induces cell cycle arrest and apoptosis in human leukemic U937 cells through Bcl-2/Bax regulation. Environ. Toxicol. Pharmacol. 2008, 26:61-67.
23. Nie F., Zhang X., Qi Q., Yang L., Yang Y., Liu W., Lu N., Wu Z., You Q., Guo Q. Reactive oxygen species accumulation contributes to gambogic acid-induced apoptosis in human hepatoma SMMC-7721 cells. Toxicology 2009, 260:60-67.
24. Nigam M., Ranjan V., Srivastava S., Sharma R., Balapure A.K. Centchroman induces G0/G1 arrest and caspase-dependent apoptosis involving mitochondrial membrane depolarization in MCF-7 and MDA MB-231 human breast cancer cells. Life Sci. 2008, 82:577-590.
25. Olson J.M., Hallahan A.R. p38 MAP kinase: a convergence point in cancer therapy. Trends Mol. Med. 2004, 10:125-129.
26. Seidman R., Gitelman I., Sagi O., Horwitz S.B., Wolfson M. The role of ERK 1/2 and p38 MAP-kinase pathways in taxol-induced apoptosis in human ovarian carcinoma cells. Exp. Cell Res. 2001, 268:84-92.
27. Shabnam M.S., Srinivasan R., Wali A., Majumdar S., Joshi K., Behera D. Expression of p53 protein and the apoptotic regulatory molecules Bcl-2, Bcl-XL, and Bax in locally advanced squamous cell carcinoma of the lung. Lung Cancer 2004, 45:181-188.
28. Sharifah Sakinah S., Tri Handayani S., Azimahtol Hawariah L. Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio. Cancer Cell Int. 2007, 7:4.
29. Tiwari M., Kakkar P. Plant derived antioxidants-geraniol and camphene protect rat alveolar macrophages against t-BHP induced oxidative stress. Toxicol. In Vitro 2009, 23:295-301.
30. Tsagarakis N.J., Drygiannakis I., Batistakis A.G., Kolios G., Kouroumalis E.A. A concentration-dependent effect of ursodeoxycholate on apoptosis and caspases activities of HepG2 hepatocellular carcinoma cells. Eur. J. Pharmacol. 2010, 640:1-7.
31. Tsuruo T., Naito M., Tomida A., Fujita N., Mashima T., Sakamoto H., Haga N. Molecular targeting therapy of cancer: drug resistance, apoptosis and survival signal. Cancer Sci. 2003, 94:15-21.
32. Whang-Peng J., Cheng A.-L., Hsu C., Chen C.-M. Clinical development and future direction for the treatment of hepatocellular carcinoma. J. Exp. Clin. Med. 2010, 2:93-103.
33. Yadavilli S., Martinez-Ceballos E., Snowden-Aikens J., Hurst A., Joseph T., Albrecht T., Muganda P.M. Diepoxybutane activates the mitochondrial apoptotic pathway and mediates apoptosis in human lymphoblasts through oxidative stress. Toxicol. In Vitro 2007, 21:1429-1441.
34. Yan F., Wang M., Chen H., Su J., Wang X., Wang F., Xia L., Li Q. Gambogenic acid mediated apoptosis through the mitochondrial oxidative stress and inactivation of Akt signaling pathway in human nasopharyngeal carcinoma CNE-1 cells. Eur. J. Pharmacol. 2011, 652:23-32.
35. Yeh C.T., Yen G.C. Effect of sulforaphane on metallothionein expression and induction of apoptosis in human hepatoma HepG2 cells. Carcinogenesis 2005, 26:2138-2148.
36. Zhao M., Guo T., Wang M., Zhao Q., Liu Y., Sun X., Wang H., Hou Y. The course of uncarinic acid E-induced apoptosis of HepG2 cells from damage to DNA and p53 activation to mitochondrial release of cytochrome c. Biol. Pharm. Bull. 2006, 29:1639-1644.