Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide

Cell Biology International

Volumn 30, Issue 11, 2006, Pages 924-932

  Baskić, Dejan ^a   Popović, Suzana ^a   Ristić, Petar ^b   Arsenijević, Nebojša N ^a  

^a UNIVERSITY OF KRAGUJEVAC   (Serbia)

^b Institute of Public Health of Montenegro   (Serbia)

Author keywords

Apoptosis; Cycloheximide; Leukocytes; Method; Microscopy

Indexed keywords

ACRIDINE ORANGE; CYCLOHEXIMIDE; ETHIDIUM BROMIDE; GENOMIC DNA; LIPOCORTIN 5; PROPIDIUM IODIDE; TRYPAN BLUE;

AGAR GEL ELECTROPHORESIS; APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL TYPE; CELL VIABILITY; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; INCUBATION TIME; INTERMETHOD COMPARISON; LEUKOCYTE; MICROSCOPY; MORPHOLOGICAL TRAIT; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; POLYMORPHONUCLEAR CELL; REPRODUCIBILITY; STAINING; ULTRAVIOLET B RADIATION;

EID: 33750354408     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cellbi.2006.06.016     Document Type: Article

References (36)

1. Arends M.J., and Wyllie A.H. Apoptosis: mechanisms and roles in pathology. Int Rev Exp Pathol 32 (1991) 223-254
2. Bossy-Wetzel E., and Green D.R. Detection of apoptosis by annexin-V. Methods Enzymol 322 (2000) 15-39
3. Brach M.A., Devos S., Gruss H.J., and Herrmann F. Prolongation of survival of human polymorphonuclear neutrophils by granulocyte-macrophage colony-stimulating factor is caused by inhibition of programmed cell death. Blood 80 (1992) 2920-2924
4. Brunetti M., Martelli N., Colasante A., Piantelli M., Musiani P., and Aiello F.B. Spontaneous and glucocorticoid-induced apoptosis in human mature T lymphocytes. Blood 86 (1995) 4199-4204
5. Chacon-Cruz E., Oelberg D.G., Davis P., and Buescher E.S. Membrane depolarization and depletion of intracellular calcium stores are associated with delay of apoptosis in human neutrophils. J Leukoc Biol 64 (1998) 759-766
6. Collins R.J., Harmon B.V., Souvlis T., Pope J.H., and Kerr J.F. Effects of cycloheximide on B-chronic lymphocytic leukemic and normal lymphocytes in vitro: induction of apoptosis. Br J Cancer 64 (1991) 518-522
7. Collins R.J., Harmon B.V., Gobe B.C., and Kerr F. Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. Int J Radiat Biol 61 (1992) 451-453
8. Columbano L., Coni P., Faa G., Manenti G., and Columbano A. Rapid induction of apoptosis in rat liver by cycloheximide. Am J Pathol 140 (1992) 545-549
9. Frankfurt O.S., Seckinger D., and Sugarbaker E.V. Apoptosis and growth inhibition in sensitive and resistant leukemic cells treated with anticancer drugs. Int J Oncol 4 (1994) 481-489
10. Gasiorowski K., Brokos B., Kulma A., Ogorzalek A., and Skorkowska K. A comparison of the methods applied to detect apoptosis in genotoxically-damaged lymphocytes cultured in the presence of four antimutagens. Cell Mol Biol Lett 6 (2001) 141-159
11. Cohen G.M., Sun X.M., Snowden R.T., Dinsdale D., and Skilleter D.N. Key morphological features of apoptosis may occur in the absence of internucleosomal fragmentation. Biochem J 286 (1992) 331-334
12. Golstein P., Ojcius D.M., and Young J.D. Cell death mechanisms and the immune system. Immunol Rev 121 (1991) 29-65
13. Gupta S., Aggarwal S., and Nguyen T. Increased spontaneous apoptosis in T lymphocytes in DiGeorge anomaly. Clin Exp Immunol 113 (1998) 65-71
14. Cohen J.J., Duke R.C., Fadok V.A., and Sellins K.S. Apoptosis and programmed cell death in immunity. Annu Rev Immunol 10 (1992) 267-293
15. Kipps T.J. Chronic lymphocytic leukemia. Curr Opin Hematol 5 (2000) 244-253
16. Krammer P.H., Behrman N., Daniel P., Dhein J., and Debatin K.M. Regulation of apoptosis in the immune system. Curr Opin Immunol 6 (1994) 279-289
17. Leite M., Quinta-Costa M., Leite P.S., and Guimaraes J.E. Critical evaluation of techniques to detect and measure cell death-study in a model of UV radiation of the leukemic cell line HL60. Anal Cell Pathol 19 (1999) 139-151
18. Lemaire C., Andreau K., Souvannavong V., and Adam A. Specific dual effect of cycloheximide on B lymphocyte apoptosis: involvement of CPP32/Caspase-3. Biochem Pharmacol 58 (1999) 85-93
19. Marcu M., Gunter H.E., Jugdutt B.I., and Docherty J. Inhibition of apoptosis after ischemia-reperfusion in rat myocardium by cycloheximide. J Mol Cell Cardiol 31 (1999) 1073-1082
20. Nagami K., Kawashima Y., Kuno H., Kemi M., and Matsumoto H. In vitro cytotoxicity assay to screen compounds for apoptosis-inducing potential on lymphocytes and neutrophils. J Toxicol Sci 27 (2002) 191-203
21. Panayiotidis P., Ganeshaguru K., Jabbar S.A.B., and Hoffbrand A.V. Interleukin-4 inhibits apoptotic cell death and loss of bcl-2 protein in B chronic lymphocytic leukemia cells in vitro. Br J Haematol 86 (1993) 169-173
22. Plasier B., Lloyd D.R., Paul G.C., Thomas C.R., and Al-Rubeai M. Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay. J Immunol Methods 229 (1999) 81-95
23. Potter A., Kim C., Gollahon K.A., and Rabinovitch P.S. Apoptotic human lymphocytes have diminished CD4 and CD8 receptor expression. Cell Immunol 193 (1999) 36-47
24. Savitskiy V.P., Shman T., and Potapnev M.P. Comparative measurament of spontaneous apoptosis in pediatric acute leukemia by different techniques. Cytometry 56 (2003) 16-22
25. Schulze-Osthoff K., Ferrari D., Los M., Wesselborg S., and Peter M.E. Apoptosis signaling by death receptors. Eur J Biochem 254 (1998) 439-459
26. Schutte B., Nuydens R., Geerts H., and Ramaekers F. Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. J Neurosci Methods 86 (1998) 63-69
27. Squier M.K., Sehnert A.J., Sellins K.S., Malkinson A.M., Takano E., and Cohen J.J. Calpain and calpastatin regulate neutrophil apoptosis. J Cell Physiol 178 (1999) 311-319
28. Stein G.M., Pfuller U., Schietzel M., and Bussing A. Expression of interleukin-4 in apoptotic cells: stimulation of the type-2 cytokine by different toxins in human peripheral blood mononuclear and tumor cells. Cytometry 41 (2000) 261-270
29. Stewart B.W. Mechanisms of apoptosis: integration of genetic, biochemical and cellular indicators. J Natl Cancer Inst 86 (1994) 1286-1296
30. Suda T., Hashimoto H., Tanaka M., Ochi T., and Nagata S. Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing. J Exp Med 186 (1997) 2045-2050
31. Sullivan G.W., Gelrud A.K., Carper H.T., and Mandell G.L. Interaction of tumor necrosis factor-alpha and granulocyte colony-stimulating factor on neutrophil apoptosis, receptor expression, and bactericidal function. Proc Assoc Am Physicians 108 (1996) 455-466
32. Tessitore L., Tomasi C., and Greco M. Fasting-induced apoptosis in rat liver is blocked by cycloheximide. Eur J Cell Biol 78 (1999) 573-579
33. Ucker D.S., Ashwell J.D., and Nickas G. Activation-driven T cell death. I. Requirement for de novo transcription and translation and association with genome fragmentation. J Immunol 143 (1989) 3461-3469
34. Vaux D.L., and Korsmeyer S.J. Cell death in development. Cell 96 (1999) 245-254
35. Vermes I., Haanen C., Steffens-Nakken H., and Reutelingsperger C. A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V. J Immunol Methods 184 (1995) 39-51
36. Vilpo J., Koski T., and Vilpo L. Calcium antagonists potentiate P-glycoprotein-independent anticancer drugs in chronic lymphocytic leukemia cells in vitro. Haematologica 85 (2000) 806-813