Growth and productivity impacts of periplasmic nuclease expression in an Escherichia coli Fab' fragment production strain

Biotechnology and Bioengineering

Volumn 109, Issue 2, 2012, Pages 517-527

  Nesbeth, Darren N ^a   Perez Pardo, Miguel Angel ^a   Ali, Shaukat ^a   Ward, John ^a   Keshavarz Moore, Eli ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Cell engineering; Growth kinetics; Nuclease; Periplasm; Productivity

Indexed keywords

BIOPROCESSES; BIOPROCESSING; CELL DISRUPTION; CELL ENGINEERING; CELL INTEGRITY; CLARIFICATION PERFORMANCE; CO-EXPRESSION; E. COLI; FAB' FRAGMENT; GENOMIC DNA; GROWTH PERFORMANCE; HIGH MOLECULAR WEIGHT; HOST CELLS; NUCLEASE; PERIPLASM; PRODUCT MANUFACTURABILITY; PRODUCTION STRAIN; PRODUCTIVITY IMPACTS; SCALE-DOWN; STAPHYLOCOCCAL NUCLEASE; VOLUMETRIC YIELD;

ANIMAL CELL CULTURE; CELLS; CLARIFIERS; DNA; ESCHERICHIA COLI; FEEDSTOCKS; GENES; HYDROLYSIS; PRODUCTIVITY; VISCOSITY;

GROWTH KINETICS;

CALCIUM ION; GENOMIC DNA; IMMUNOGLOBULIN F(AB) FRAGMENT; NUCLEASE;

AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; BIOENGINEERING; CELL DENSITY; CELL DISRUPTION; CELL GROWTH; CYTOPLASM; ENZYME ACTIVITY; ESCHERICHIA COLI; FED BATCH FERMENTATION; GROWTH RATE; HOST CELL; HYDROLYSIS; IMMUNOGLOBULIN PRODUCTION; KINETICS; NONHUMAN; PLASMID; PRODUCTIVITY; PROTEIN EXPRESSION; STEADY STATE; VISCOSITY;

CELL ENGINEERING; CLONING, MOLECULAR; ESCHERICHIA COLI; FERMENTATION; IMMUNOGLOBULIN FAB FRAGMENTS; KINETICS; MICROCOCCAL NUCLEASE; PERIPLASM; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 83555161729     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23316     Document Type: Article

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