Evaluation of the effects and interactions of mixing and oxygen transfer on the production of Fab' antibody fragments in Escherichia coli fermentation with gas blending

Bioprocess and Biosystems Engineering

Volumn 27, Issue 6, 2005, Pages 365-374

  García Arrazola, Roeb ^a   Dawson, Pierre ^a   Buchanan, Ian ^a   Doyle, Billy ^a   Fearn, Tom ^a   Titchener Hooker, Nigel ^a   Baganz, Frank ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ESCHERICHIA COLI; FERMENTATION; MIXING; OXYGEN;

CELL DENSITIES; DISSOLVED OXYGEN TENSION (DOT); GAS BLENDING;

ANTIBODIES;

ANTIBODY;

AGITATION; ARTICLE; CELL DENSITY; CYTOPLASM; DISSOLUTION; ESCHERICHIA COLI; FERMENTATION; GAS ANALYSIS; INDUSTRY; NONHUMAN; OXYGEN TENSION; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; BIOLOGICAL TRANSPORT, ACTIVE; BIOREACTORS; CELL PROLIFERATION; COMPUTER SIMULATION; ESCHERICHIA COLI; GASES; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MICROFLUIDICS; MODELS, BIOLOGICAL; OXYGEN; OXYGEN CONSUMPTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 28244480375     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-005-0414-4     Document Type: Article

References (40)

1. Alexeeva S, Hellingwerf KJ, Teixeira de Mattos MJ (2002) Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli. J Bacteriol 184(5):1402-1406
2. Amanullah A, Tuttiet B, Nienow AW (1998) Agitator speed and dissolved oxygen effects in Xanthan fermentations. Biotech Bioeng 57(2):198-210
3. Andersson L, Yang S, Neubauer P, Enfors SO (1996) Impact of plasmid presence and induction on cellular response in fedbacth cultures of Escherichia coli. J Biotechnol 46:255-263
4. Andersson L, Strandberg L, Enfors SO (1996) Cell segregation and lysis have profound effects on the growth of Escherichia coli in high cell density fed batch cultures. Biotechnol Prog 12:190-195
5. Bailey JE, Ollis DF (1986) Biochemical engineering fundamentals, 2nd edn. McGraw-Hill International editions, Singapore
6. Bhattacharya SK, Dubey AK (1997) Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli. Enzyme Microb Technol 20:355-360
7. Berry MJ, Wattam TAK, Willets J, Linder N, De Graaf T, Hunt T, Gani M, Davis PJ, Porter P (1994) Assay and purification of Fv fragments in fermenter cultures: design and evaluation of generic binding reagents. J Immunol Methods 167:173-182
8. Bowering LC, Bracewell DG, Keshavarz-Moore E, Hoare M, Neil A, Weir C (2002) Comparison of techniques for monitoring antibody fragment production in E. coli fermentation cultures. Biotechnol Prog 18:1431-1438
9. Brekke OH, Loset GA (2003) New technologies in therapeutic antibody development. Curr Opin Pharmacol 3:544-550
10. Carter P, Kelly RF, Rodriguez ML, Snedcor B, Covarrubias M, Velligan MD, Wong WLT, Rowland AM, Kotts CE, Carver ME, Yang M, Bourrel JH, Shepard HM, Henner D (1992) High level E. coli expression and production of a bivalent huminizes antibody fragment. Bio Technol 10:163-167
11. De Leon A, Hernandez V, Galindo E, Ramirez OT (2003) Effects of dissolved oxygen tension on the production of recombinant penicillin acylase in Escherichia coli. Enzyme Microb Tech 33:689-697
12. El-Mansi EM, Holms WH (1989) Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous cultures. J Gen Microbiol 135(Pt 11):2875-2883
13. Ferguson GP, Tötemeyer S, MacLean MJ, Booth IR (1998) Methylglyoxal production in bacteria: suicide or survival? Arch Microbiol 170:209-219
14. Flores F, Torres LG, Galindo E (1994) Effect of the dissolved-oxygen tension during cultivation of Xanthomonas campestris on the production and quality of Xanthan gum. J Biotech 34(2):165-173
15. Gibbs PA, Seviour RJ (1996) Does agitation rate and/or oxygen saturation influence exopolysaccharide production by Aurebasidium pullulans in batch culture? Appl Microbiol Biotechnol 46:503-510
16. Gupta A, Rao G (2003) A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnol Bioeng 5(843):351-358
17. Harris B (1999) Exploiting antibody-based technologies to manage environmental pollution. Trends Biotech 17(7):290-296
18. Harrison JS, Keshavarz-Moore E (1996) Production of antibody fragments in Escherichia coli. Recombinant DNA biotechnology III: the integration of biological and engineering sciences 182
19. Holms WH (1986) The central metabolic pathways of E. coli relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion to acetate. Curr Top Cell Reg 28:69-105
20. Holms WH (1996) Flux analysis and control of the central metabolic pathways in E. coli. Microb Rev 19:85-116
21. Hogema BM, Arents JC, Baden R, Postma P (1999) Autoregulation of lactose uptake through the LacYpermease by enzyme UA Glc of the PTS in Escherichia coli K12. Mol Microb 31:1825-1833
22. Horn U, Strittmatter W, Krebber A, Knupfer U, Kujau M, Wenderoth R, Muller K, Matzku S, Pluckthun A, Riesenberg D (1996) High volumetric yields of functional dimeric antibodies miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions. Appl Microbiol Biotechnol 46:524-532
23. Humphreys DP, Carrington B, Bowering LC, Ganesh R, Sehdev M, Smith BJ, King LM, Reeks DG, Lawson A, Popplewell AG (2002) A plasmid system for optimization of Fab' production in Escherichia coli: importance of balance of heavy chain and light chain synthesis. Protein Expr Purif 26(2):309-320
24. Johnston W, Cord-Ruwisch R, Cooney MJ (2002) Industrial control of recombinant E. coli fed-batch culture: new perspectives on traditional controlled variables. Bioprocess Biosystems Eng 25:111-120
25. King DJ, Byron OD, Mountain A, Weir N, Harvey A, Lawson DG, Proudfoot KA, Baldock D, Harding E, Yarranton GT, Owens RJ (1993) Expression, purification and characterisation of B72.3 Fv fragments. Biochem J 290:723-729
26. Korz DJ, Rinas U, Hellmuth K, Sanders E A, Deckwer W-D (1995) Simple fed-batch technique for high cell density cultivation of Escherichia coli. J Biotech 39:59-65
27. Lee SY (1996) High cell-density culture of Escherichia coli. Trends Biotechnol 14:98-105
28. Li X, Robbins JW Jr, Taylor KB (1992) Effect of the levels of dissolved oxygen on the expression of recombinant proteins in four recombinant Escherichia coli strains. J Ind Microbiol 9(1):1-9
29. Lodish H, Berk A, Lawrence Zipursky S, Matsudaira P, Baltimore D, Darnell J (2000) Molecular cell biology, 4th edn. Freeman and Company, New York
30. Luli G, Strohl WR (1990) Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. App Environ Microbiol 56(4):1004-1011
31. Macaloney G, Hall JW, Rollins MJ, Draper I, Anderson KB, Preston J, Thompson BG, McNeil B (1997) The utility and performance of near-infra red spectroscopy in simultaneous monitoring of multiple components in a high cell density recombinant Escherichia coli production process. Bioprocess Eng 17:157-167
32. Montgomery DC (2001) Design and analysis of experiments, 5th edn. Wiley, USA
33. Neubauer P, Lin HY, Mathiszik B (2003) Metabolic load of recombinant protein production: inhibition of cellular capacities for glucose uptake and respiration after induction of a heterologous gene in Escherichia coli. Biotech Bioeng 83:53-64
34. O'Bierne D, Hammer G (2000) Oxygen availability and the growth of Escherichia coli W3110: a problem exacerbated by scale-up. Bioprocess Biosyst Eng 23:487-494
35. Pollard DJ, Kirschner TF, Hernandez D, Hunt G, Olewinski R, Salmon PM (2002) Pilot-scale process sensitivity studies for the scale-up of fungal fermentation for the production of pneumocandis. Biotech Bioeng 78(3):270-279
36. Roque AC, Lowe CR, Taipa M (2004) Antibodies and genetically engineered related molecules production and purification. Biotechnol Prog 20:639-654
37. Rocha I, Ferreira EC (2002) On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli. Anal Chim Acta 462:293-304
38. Sanden AM, Prytz I, Tubulekas I, Forberg C, Le H, Hektor A, Neubauer P, Pragai Z, Harwood C, Ward A, Picon A, De Mattos JT, Postma P, Farewell A, Nystrom T, Reeh S, Pedersen S, Larsson G (2003) Limiting factors in Escherichia coli fed-batch production of recombinant proteins. Biotechnol Bioeng 20;81(2):158-166
39. Straight J, Parulekar S, Jansen N (1989) Bacterial growth on lactose: an experimental investigation. Biotechnol Bioeng 34:705-716
40. Vaara M (1992) Agents that increase the permeability of the outer membrane. Microbiol Rev 56:395-411