-
1
-
-
0022751283
-
Site-directed mutagenesis
-
Carter P (1986) Site-directed mutagenesis. Biochem J 237: 1-7
-
(1986)
Biochem J
, vol.237
, pp. 1-7
-
-
Carter, P.1
-
2
-
-
0029683933
-
Site-directed mutagenesis using a rapid PCR-based method
-
Costa GL, Bauer JC, McGowan B, Angert M, Weiner MP (1996) Site-directed mutagenesis using a rapid PCR-based method. Methods Mol Biol 57: 239-248
-
(1996)
Methods Mol Biol
, vol.57
, pp. 239-248
-
-
Costa, G.L.1
Bauer, J.C.2
McGowan, B.3
Angert, M.4
Weiner, M.P.5
-
3
-
-
0030048583
-
Construction and evolution of antibodyphage libraries by DNA shuffling
-
Crameri A, Cwirla S, Stemmer WP (1996) Construction and evolution of antibodyphage libraries by DNA shuffling. Nat Med 2: 100-102
-
(1996)
Nat Med
, vol.2
, pp. 100-102
-
-
Crameri, A.1
Cwirla, S.2
Stemmer, W.P.3
-
5
-
-
0031574019
-
Approaches to DNA mutagenesis: An overview
-
Ling MM, Robinson BH (1997) Approaches to DNA mutagenesis: an overview. Anal Biochem 254: 157-178
-
(1997)
Anal Biochem
, vol.254
, pp. 157-178
-
-
Ling, M.M.1
Robinson, B.H.2
-
6
-
-
3142685154
-
In-vitro protein evolution by ribosome display and mRNA display
-
Lipovsek D, Plückthun A (2004) In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods 290: 51-67
-
(2004)
J Immunol Methods
, vol.290
, pp. 51-67
-
-
Lipovsek, D.1
Plückthun, A.2
-
7
-
-
33747356025
-
Latest technologies for the enhancement of antibody affinity
-
Wark KL, Hudson PJ (2006) Latest technologies for the enhancement of antibody affinity. Adv Drug Deliv Rev 58: 657-670
-
(2006)
Adv Drug Deliv Rev
, vol.58
, pp. 657-670
-
-
Wark, K.L.1
Hudson, P.J.2
-
9
-
-
36749056244
-
Dissecting protein structure and function using directed evolution
-
Yuen CM, Liu DR (2007) Dissecting protein structure and function using directed evolution. Nat Methods 4: 995-997
-
(2007)
Nat Methods
, vol.4
, pp. 995-997
-
-
Yuen, C.M.1
Liu, D.R.2
-
10
-
-
0029043683
-
Antibody VH domains as small recognition units
-
Davies J, Riechmann L (1995) Antibody VH domains as small recognition units. Biotechnology (N Y) 13: 475-479
-
(1995)
Biotechnology (N Y)
, vol.13
, pp. 475-479
-
-
Davies, J.1
Riechmann, L.2
-
11
-
-
27144432842
-
Engineered antibody fragments and the rise of single domains
-
Holliger P, Hudson PJ (2005) Engineered antibody fragments and the rise of single domains. Nat Biotechnol 23: 1126-1136
-
(2005)
Nat Biotechnol
, vol.23
, pp. 1126-1136
-
-
Holliger, P.1
Hudson, P.J.2
-
12
-
-
0142080390
-
Domain antibodies: Proteins for therapy
-
Holt LJ, Herring C, Jespers LS, Woolven BP, Tomlinson IM (2003) Domain antibodies: proteins for therapy. Trends Biotechnol 21: 484-490
-
(2003)
Trends Biotechnol
, vol.21
, pp. 484-490
-
-
Holt, L.J.1
Herring, C.2
Jespers, L.S.3
Woolven, B.P.4
Tomlinson, I.M.5
-
14
-
-
54849403487
-
Single-domain antibodies as building blocks for novel therapeutics
-
Saerens D, Ghassabeh GH, Muyldermans S (2008) Single-domain antibodies as building blocks for novel therapeutics. Curr Opin Pharmacol 8: 600-608
-
(2008)
Curr Opin Pharmacol
, vol.8
, pp. 600-608
-
-
Saerens, D.1
Ghassabeh, G.H.2
Muyldermans, S.3
-
15
-
-
64949167425
-
Selection of non-aggregating VH binders from synthetic VH phage display libraries
-
Arbabi-Ghahroudi M, MacKenzie R, Tanha J (2009) Selection of non-aggregating VH binders from synthetic VH phage display libraries. Methods Mol Biol 525: 187-216, xiii
-
(2009)
Methods Mol Biol
, vol.525
, Issue.187-216
-
-
Arbabi-Ghahroudi, M.1
MacKenzie, R.2
Tanha, J.3
-
16
-
-
61849131077
-
Aggregation-resistant VHs selected by in vitro evolution tend to have disulfidebonded loops and acidic isoelectric points
-
Arbabi-Ghahroudi M, To R, Gaudette N, Hirama T, Ding W, MacKenzie R, Tanha J (2009) Aggregation-resistant VHs selected by in vitro evolution tend to have disulfidebonded loops and acidic isoelectric points. Protein Eng Des Sel 22: 59-66
-
(2009)
Protein Eng des Sel
, vol.22
, pp. 59-66
-
-
Arbabi-Ghahroudi, M.1
To, R.2
Gaudette, N.3
Hirama, T.4
Ding, W.5
MacKenzie, R.6
Tanha, J.7
-
17
-
-
50149083288
-
Construction of a large phagedisplayed human antibody domain library with a scaffold based on a newly identified highly soluble, stable heavy chain variable domain
-
Chen W, Zhu Z, Feng Y, Xiao X, Dimitrov DS (2008) Construction of a large phagedisplayed human antibody domain library with a scaffold based on a newly identified highly soluble, stable heavy chain variable domain. J Mol Biol 382: 779-789
-
(2008)
J Mol Biol
, vol.382
, pp. 779-789
-
-
Chen, W.1
Zhu, Z.2
Feng, Y.3
Xiao, X.4
Dimitrov, D.S.5
-
18
-
-
4444302074
-
Aggregation-resistant domain antibodies selected on phage by heat denaturation
-
Jespers L, Schon O, Famm K, Winter G (2004) Aggregation-resistant domain antibodies selected on phage by heat denaturation. Nat Biotechnol 22: 1161-1165
-
(2004)
Nat Biotechnol
, vol.22
, pp. 1161-1165
-
-
Jespers, L.1
Schon, O.2
Famm, K.3
Winter, G.4
-
19
-
-
33750287537
-
Improving solubility and refolding efficiency of human VHs by a novel mutational approach
-
Tanha J, Nguyen TD, Ng A, Ryan S, Ni F, MacKenzie R (2006) Improving solubility and refolding efficiency of human VHs by a novel mutational approach. Protein Eng Des Sel 19: 503-509
-
(2006)
Protein Eng des Sel
, vol.19
, pp. 503-509
-
-
Tanha, J.1
Nguyen, T.D.2
Ng, A.3
Ryan, S.4
Ni, F.5
MacKenzie, R.6
-
20
-
-
34548810380
-
Repertoires of aggregation-resistant human antibody domains
-
Christ D, Famm K, Winter G (2007) Repertoires of aggregation-resistant human antibody domains. Protein Eng Des Sel 20: 413-416
-
(2007)
Protein Eng des Sel
, vol.20
, pp. 413-416
-
-
Christ, D.1
Famm, K.2
Winter, G.3
-
21
-
-
39049173446
-
Thermodynamically stable aggregation- resistant antibody domains through directed evolution
-
Famm K, Hansen L, Christ D, Winter G (2008) Thermodynamically stable aggregation- resistant antibody domains through directed evolution. J Mol Biol 376: 926-931
-
(2008)
J Mol Biol
, vol.376
, pp. 926-931
-
-
Famm, K.1
Hansen, L.2
Christ, D.3
Winter, G.4
-
22
-
-
0035816720
-
Optimal design features of camelized human singledomain antibody libraries
-
Tanha J, Xu P, Chen ZG, Ni F, Kaplan H, Narang SA, MacKenzie CR (2001) Optimal design features of camelized human singledomain antibody libraries. J Biol Chem 276: 24774-24780
-
(2001)
J Biol Chem
, vol.276
, pp. 24774-24780
-
-
Tanha, J.1
Xu, P.2
Chen, Z.G.3
Ni, F.4
Kaplan, H.5
Narang, S.A.6
MacKenzie, C.R.7
-
23
-
-
29244442688
-
Isolation of monomeric human VHs by a phage selection
-
To R, Hirama T, Arbabi-Ghahroudi M, MacKenzie R, Wang P, Xu P, Ni F, Tanha J (2005) Isolation of monomeric human VHs by a phage selection. J Biol Chem 280: 41395-41403
-
(2005)
J Biol Chem
, vol.280
, pp. 41395-41403
-
-
To, R.1
Hirama, T.2
Arbabi-Ghahroudi, M.3
MacKenzie, R.4
Wang, P.5
Xu, P.6
Ni, F.7
Tanha, J.8
-
24
-
-
77957219390
-
A simple method for site-directed mutagenesis using the polymerase chain reaction
-
Hemsley A, Arnheim N, Toney MD, Cortopassi G, Galas DJ (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res 17: 6545-6551
-
(1989)
Nucleic Acids Res
, vol.17
, pp. 6545-6551
-
-
Hemsley, A.1
Arnheim, N.2
Toney, M.D.3
Cortopassi, G.4
Galas, D.J.5
-
25
-
-
0023684064
-
Ageneral method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interactions
-
Higuchi R, Krummel B, Saiki RK (1988) Ageneral method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 16: 7351-7367
-
(1988)
Nucleic Acids Res
, vol.16
, pp. 7351-7367
-
-
Higuchi, R.1
Krummel, B.2
Saiki, R.K.3
-
26
-
-
0024520745
-
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
-
Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59
-
(1989)
Gene
, vol.77
, pp. 51-59
-
-
Ho, S.N.1
Hunt, H.D.2
Horton, R.M.3
Pullen, J.K.4
Pease, L.R.5
-
27
-
-
0024323295
-
A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase chain reaction
-
Nelson RM, Long GL (1989) A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase chain reaction. Anal Biochem 180: 147-151
-
(1989)
Anal Biochem
, vol.180
, pp. 147-151
-
-
Nelson, R.M.1
Long, G.L.2
-
28
-
-
0024507215
-
Construction of mutant and chimeric genes using the polymerase chain reaction
-
Vallette F, Mege E, Reiss A, Adesnik M (1989) Construction of mutant and chimeric genes using the polymerase chain reaction. Nucleic Acids Res 17: 723-733
-
(1989)
Nucleic Acids Res
, vol.17
, pp. 723-733
-
-
Vallette, F.1
Mege, E.2
Reiss, A.3
Adesnik, M.4
-
29
-
-
0036006146
-
Functional characterization of an anti-estradiol antibody by site-directed mutagenesis and molecular modeling: Modulation of binding properties and prominent role of the VL domain in estradiol recognition
-
Coulon S, Pellequer JL, Blachere T, Chartier M, Mappus E, Chen SW, Cuilleron CY, Baty D (2002) Functional characterization of an anti-estradiol antibody by site-directed mutagenesis and molecular modeling: modulation of binding properties and prominent role of the VL domain in estradiol recognition. J Mol Recognit 15: 6-18
-
(2002)
J Mol Recognit
, vol.15
, pp. 6-18
-
-
Coulon, S.1
Pellequer, J.L.2
Blachere, T.3
Chartier, M.4
Mappus, E.5
Chen, S.W.6
Cuilleron, C.Y.7
Baty, D.8
-
30
-
-
0028020642
-
A high-yield modification of mutation by overlap extension using three primers
-
Dilsiz N, Crabbe MJ (1994) A high-yield modification of mutation by overlap extension using three primers. Anal Biochem 222: 510-511
-
(1994)
Anal Biochem
, vol.222
, pp. 510-511
-
-
Dilsiz, N.1
Crabbe, M.J.2
-
31
-
-
12244251436
-
Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo
-
Dolk E, van der Vaart M, Lutje HD, Vriend G, de Haard H, Spinelli S, Cambillau C, Frenken L, Verrips T (2005) Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo. Appl Environ Microbiol 71: 442-450
-
(2005)
Appl Environ Microbiol
, vol.71
, pp. 442-450
-
-
Dolk, E.1
Van Der Vaart, M.2
Lutje, H.D.3
Vriend, G.4
De Haard, H.5
Spinelli, S.6
Cambillau, C.7
Frenken, L.8
Verrips, T.9
-
32
-
-
0028339125
-
Identification of the IgG binding site of the human low affinity receptor for IgG Fc gamma RII. Enhancement and ablation of binding by sitedirected mutagenesis
-
Hulett MD, Witort E, Brinkworth RI, McKenzie IF, Hogarth PM (1994) Identification of the IgG binding site of the human low affinity receptor for IgG Fc gamma RII. Enhancement and ablation of binding by sitedirected mutagenesis. J Biol Chem 269: 15287-15293
-
(1994)
J Biol Chem
, vol.269
, pp. 15287-15293
-
-
Hulett, M.D.1
Witort, E.2
Brinkworth, R.I.3
McKenzie, I.F.4
Hogarth, P.M.5
-
33
-
-
0029120976
-
The manganese binding site of manganese peroxidase: Characterization of an Asp179Asn site-directed mutant protein
-
Kusters-van Someren M, Kishi K, Lundell T, Gold MH (1995) The manganese binding site of manganese peroxidase: characterization of an Asp179Asn site-directed mutant protein. Biochemistry 34: 10620-10627
-
(1995)
Biochemistry
, vol.34
, pp. 10620-10627
-
-
Kusters-Van Someren, M.1
Kishi, K.2
Lundell, T.3
Gold, M.H.4
-
34
-
-
33750329048
-
A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis
-
Peng RH, Xiong AS, Yao QH (2006) A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Appl Microbiol Biotechnol 73: 234-240
-
(2006)
Appl Microbiol Biotechnol
, vol.73
, pp. 234-240
-
-
Peng, R.H.1
Xiong, A.S.2
Yao, Q.H.3
-
35
-
-
0035399396
-
A novel reagentless sensing system for measuring glucose based on the galactose/ glucose-binding protein
-
Salins LL, Ware RA, Ensor CM, Daunert S (2001) A novel reagentless sensing system for measuring glucose based on the galactose/ glucose-binding protein. Anal Biochem 294: 19-26
-
(2001)
Anal Biochem
, vol.294
, pp. 19-26
-
-
Salins, L.L.1
Ware, R.A.2
Ensor, C.M.3
Daunert, S.4
-
36
-
-
0038350454
-
Functional relationships of the sialyltransferases involved in expression of the polysialic acid capsules of Escherichia coli K1 and K92 and Neisseria meningitidis groups B or C
-
Steenbergen SM, Vimr ER (2003) Functional relationships of the sialyltransferases involved in expression of the polysialic acid capsules of Escherichia coli K1 and K92 and Neisseria meningitidis groups B or C. J Biol Chem 278: 15349-15359
-
(2003)
J Biol Chem
, vol.278
, pp. 15349-15359
-
-
Steenbergen, S.M.1
Vimr, E.R.2
-
37
-
-
0030896669
-
Mutational analysis of the adeno-associated virus Rep68 protein: Identification of critical residues necessary for site-specific endonuclease activity
-
Walker SL, Wonderling RS, Owens RA (1997) Mutational analysis of the adeno-associated virus Rep68 protein: identification of critical residues necessary for site-specific endonuclease activity. J Virol 71: 2722-2730
-
(1997)
J Virol
, vol.71
, pp. 2722-2730
-
-
Walker, S.L.1
Wonderling, R.S.2
Owens, R.A.3
-
38
-
-
0031714699
-
Interferon-gamma variants with deletions in the AB surface loop. Flexibility is a critical point for receptor binding
-
Waschutza G, Dengler U, Villmann C, Bottinger H, Otto B (1998) Interferon-gamma variants with deletions in the AB surface loop. Flexibility is a critical point for receptor binding. Eur J Biochem 256: 303-309
-
(1998)
Eur J Biochem
, vol.256
, pp. 303-309
-
-
Waschutza, G.1
Dengler, U.2
Villmann, C.3
Bottinger, H.4
Otto, B.5
-
39
-
-
0024556150
-
Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
-
Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77: 61-68
-
(1989)
Gene
, vol.77
, pp. 61-68
-
-
Horton, R.M.1
Hunt, H.D.2
Ho, S.N.3
Pullen, J.K.4
Pease, L.R.5
-
40
-
-
0028966467
-
Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen
-
Dan MD, Earley EM, Griffin MC, Maiti PK, Prashar AK, Yuan XY, Friesen AD, Kaplan HA (1995) Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen. J Neurosurg 82: 475-480
-
(1995)
J Neurosurg
, vol.82
, pp. 475-480
-
-
Dan, M.D.1
Earley, E.M.2
Griffin, M.C.3
Maiti, P.K.4
Prashar, A.K.5
Yuan, X.Y.6
Friesen, A.D.7
Kaplan, H.A.8
-
42
-
-
0036570726
-
Selection by phage display of llama conventional VH fragments with heavy chain antibody VHH properties
-
Tanha J, Dubuc G, Hirama T, Narang SA, MacKenzie CR (2002) Selection by phage display of llama conventional VH fragments with heavy chain antibody VHH properties. J Immunol Methods 263: 97-109
-
(2002)
J Immunol Methods
, vol.263
, pp. 97-109
-
-
Tanha, J.1
Dubuc, G.2
Hirama, T.3
Narang, S.A.4
MacKenzie, C.R.5
-
43
-
-
0028933653
-
A modified medium for efficient electrotransformation of
-
Tung WL, Chow KC (1995) A modified medium for efficient electrotransformation of E. coli. Trends Genet 11: 128-129
-
(1995)
E. Coli. Trends Genet
, vol.11
, pp. 128-129
-
-
Tung, W.L.1
Chow, K.C.2
-
44
-
-
0025735582
-
Synthesis and expression in Escherichia coli of cistronic DNA encoding an antibody fragment specific for a Salmonella serotype B O-antigen
-
Anand NN, Dubuc G, Phipps J, MacKenzie CR, Sadowska J, Young NM, Bundle DR, Narang SA (1991) Synthesis and expression in Escherichia coli of cistronic DNA encoding an antibody fragment specific for a Salmonella serotype B O-antigen. Gene 100: 39-44
-
(1991)
Gene
, vol.100
, pp. 39-44
-
-
Anand, N.N.1
Dubuc, G.2
Phipps, J.3
MacKenzie, C.R.4
Sadowska, J.5
Young, N.M.6
Bundle, D.R.7
Narang, S.A.8
-
45
-
-
0028280127
-
Effect of Cλ- Ck domain switching on Fab activity and yield in Escherichia coli: Synthesis and expression of genes encoding two anti-carbohydrate Fabs
-
MacKenzie CR, Sharma V, Brummell D, Bilous D, Dubuc G, Sadowska J, Young NM, Bundle DR, Narang SA (1994) Effect of Cλ- Ck domain switching on Fab activity and yield in Escherichia coli: synthesis and expression of genes encoding two anti-carbohydrate Fabs. Biotechnology (N Y) 12: 390-395
-
(1994)
Biotechnology (N Y)
, vol.12
, pp. 390-395
-
-
MacKenzie, C.R.1
Sharma, V.2
Brummell, D.3
Bilous, D.4
Dubuc, G.5
Sadowska, J.6
Young, N.M.7
Bundle, D.R.8
Narang, S.A.9
-
46
-
-
0014949207
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
-
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
-
(1970)
Nature
, vol.227
, pp. 680-685
-
-
Laemmli, U.K.1
-
47
-
-
0028871804
-
How to measure and predict the molar absorption coefficient of a protein
-
Pace CN, Vajdos F, Fee L, Grimsley G, Gray T (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci 4: 2411-2423
-
(1995)
Protein Sci
, vol.4
, pp. 2411-2423
-
-
Pace, C.N.1
Vajdos, F.2
Fee, L.3
Grimsley, G.4
Gray, T.5
-
48
-
-
1642364974
-
Crystal structure of HEL4, a soluble, refoldable human VH single domain with a germ-line scaffold
-
Jespers L, Schon O, James LC, Veprintsev D, Winter G (2004) Crystal structure of HEL4, a soluble, refoldable human VH single domain with a germ-line scaffold. J Mol Biol 337: 893-903
-
(2004)
J Mol Biol
, vol.337
, pp. 893-903
-
-
Jespers, L.1
Schon, O.2
James, L.C.3
Veprintsev, D.4
Winter, G.5
-
49
-
-
0032955291
-
Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies
-
van der Linden RHJ, Frenken LGJ, de Geus B, Harmsen MM, Ruuls RC, Stok W, de Ron L, Wilson S, Davis P, Verrips CT (1999) Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochim Biophys Acta 1431: 37-46
-
(1999)
Biochim Biophys Acta
, vol.1431
, pp. 37-46
-
-
Van Der Linden, R.H.J.1
Lgj, F.2
De Geus, B.3
Harmsen, M.M.4
Ruuls, R.C.5
Stok, W.6
De Ron, L.7
Wilson, S.8
Davis, P.9
Verrips, C.T.10
-
50
-
-
0029746203
-
Single antibody domains as small recognition units: Design and in vitro antigen selection of camelized, human VH domains with improved protein stability
-
Davies J, Riechmann L (1996) Single antibody domains as small recognition units: design and in vitro antigen selection of camelized, human VH domains with improved protein stability. Protein Eng 9: 531-537
-
(1996)
Protein Eng
, vol.9
, pp. 531-537
-
-
Davies, J.1
Riechmann, L.2
|