Construction of a Large Phage-Displayed Human Antibody Domain Library with a Scaffold Based On a Newly Identified Highly Soluble, Stable Heavy Chain Variable Domain

Journal of Molecular Biology

Volumn 382, Issue 3, 2008, Pages 779-789

  Chen, Weizao ^a   Zhu, Zhongyu ^a,b   Feng, Yang ^a   Xiao, Xiaodong ^a   Dimitrov, Dimiter S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SAIC FREDERICK INC   (United States)

Author keywords

antibody library; framework scaffold; human; phage display; VH

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; VIRUS ENVELOPE PROTEIN;

ARTICLE; BACTERIOPHAGE; COMPLEMENTARITY DETERMINING REGION; ESCHERICHIA COLI; HEAVY CHAIN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STABILITY;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS;

EID: 50149083288     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2008.07.054     Document Type: Article

References (13)

1. Ward E.S., Gussow D., Griffiths A.D., Jones P.T., and Winter G. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 341 (1989) 544-546
2. Holt L.J., Herring C., Jespers L.S., Woolven B.P., and Tomlinson I.M. Domain antibodies: proteins for therapy. Trends Biotechnol. 21 (2003) 484-490
3. Muyldermans S., Cambillau C., and Wyns L. Recognition of antigens by single-domain antibody fragments: the superfluous luxury of paired domains. Trends Biochem. Sci. 26 (2001) 230-235
4. Soderlind E., Strandberg L., Jirholt P., Kobayashi N., Alexeiva V., Aberg A.M., et al. Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries. Nat. Biotechnol. 18 (2000) 852-856
5. Lee C.V., Liang W.C., Dennis M.S., Eigenbrot C., Sidhu S.S., and Fuh G. High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold. J. Mol. Biol. 340 (2004) 1073-1093
6. Zhu Z., Dimitrov A.S., Bossart K.N., Crameri G., Bishop K.A., Choudhry V., et al. Potent neutralization of Hendra and Nipah viruses by human monoclonal antibodies. J. Virol. 80 (2006) 891-899
7. Zhang M.Y., Shu Y., Phogat S., Xiao X., Cham F., Bouma P., et al. Broadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library. J. Immunol. Methods 283 (2003) 17-25
8. H3-encoded Igs. J. Immunol. 157 (1996) 2982-2988
9. H domain of anti-neuraminidase antibody NC41. J. Protein Chem. 14 (1995) 167-178
10. Ewert S., Cambillau C., Conrath K., and Pluckthun A. Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. Biochemistry 41 (2002) 3628-3636
11. Skerra A. Alternative non-antibody scaffolds for molecular recognition. Curr. Opin. Biotechnol. 18 (2007) 295-304
12. Jirholt P., Ohlin M., Borrebaeck C.A., and Soderlind E. Exploiting sequence space: shuffling in vivo formed complementarity determining regions into a master framework. Gene 215 (1998) 471-476
13. HH domain stability and the design of monobody scaffolds for naive antibody libraries. J. Mol. Biol. 332 (2003) 643-655