Interferon-γ variants with deletions in the AB surface loop. Flexibility is a critical point for receptor binding

European Journal of Biochemistry

Volumn 256, Issue 2, 1998, Pages 303-309

  Waschütza, Gero ^a   Dengler, Uwe ^b   Villmann, Carmen ^a   Böttinger, Heiner ^c   Otto, Bernd ^a  

^a FRAUNHOFER INSTITUTE FOR INTERFACIAL ENGINEERING AND BIOTECHNOLOGY IGB   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Computer modelling; Interferon ; Protein loops

Indexed keywords

GAMMA INTERFERON; GAMMA INTERFERON RECEPTOR; HLA DR ANTIGEN; RECOMBINANT GAMMA INTERFERON;

ANTIVIRAL ACTIVITY; ARTICLE; BINDING SITE; CELL LINE; CIRCULAR DICHROISM; COLON CANCER; CONTROLLED STUDY; DELETION MUTANT; DRUG STABILITY; ENCEPHALOMYOCARDITIS VIRUS; ESCHERICHIA COLI; GENE CONSTRUCT; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IN VITRO STUDY; LUNG FIBROBLAST; MOLECULAR DYNAMICS; MOLECULAR MODEL; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RECEPTOR AFFINITY; STRUCTURE ACTIVITY RELATION;

ENCEPHALOMYOCARDITIS VIRUS; ESCHERICHIA COLI;

EID: 0031714699     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2560303.x     Document Type: Article

References (27)

1. Günther, G. & Otto, B. (1993) Interferon. An overview of the state of basic research with special regard to interferon-gamma, Arzneimittelforschung 43, 182-186.
2. Ealick, S. E., Cook, W. J., Vijay-Kumar, S., Carson, M., Nagabhushan, T. L., Trotta, P. P. & Bugg, C. E. (1991) Three-dimensional structure of recombinant human interferon-gamma, Science 252, 698-702.
3. Samudzi, C. T., Burton, L. E. & Rubin, J. R. (1991) Crystal structure of recombinant rabbit interferon-gamma at 2,7 Å resolution, J. Biol. Chem. 266, 21791-21797.
4. Walter, M. R., Windsor, W. T., Nagabhushan, T. L., Lundell, D. J., Lunn, C. A., Zauodny, P. J. & Narula, S. K. (1995) Crystal structure of a complex between interferon-gamma and its soluble high-affinity receptor, Nature 376, 230-235.
5. Lunn, C. A., Fossetta, J., Dalgarno, D., Murgolo, N., Windsor, W., Zauodny, P. J., Narula, S. K. & Lundell, D. (1992) A point mutation of human interferon-gamma abolishes receptor recognition, Protein Eng. 5, 253-257.
6. Waschütza, G., Li, V., Schäfer, T., Schomburg, D., Villmann, C., Zakaria, H. & Otto, B. (1996) Engineered disulfide bonds in recombinant human interferon-gamma: the impact of the N-terminal helix A and the AB loop on protein stability, Protein Eng. 9, 905-912.
7. Fetrow, J. S. (1995) Omega loops: nonregular secondary structures significant in protein function and stability, FASEB J. 9, 708-717
8. Lundell, D., Lunn, C., Senior, M., Zauodny, P. & Narula, S. (1994) Importance of the loop connecting A and B helices of human interferon-gamma in recognition by interferon-gamma receptor, J. Biol. Chem. 269, 16159-16162.
9. Schomburg, D. & Reichelt, J. (1988) BRAGI: a comprehensive protein modeling program system, J. Mol. Graph. 6, 161-168.
10. Fechteler, T., Dengler, U. & Schomburg, D. (1995) Prediction of protein three-dimensional structures in insertion and deletion regions: a procedure for searching data bases of representative protein fragments using geometric scoring criteria, J. Mol. Biol. 253, 114-131.
11. Weiner, S., Kollman, P., Nguyen, D. & Case, D. (1986) An all atom force field tor simulations of proteins and nucleic acids, J. Comp. Chem. 7, 230-252.
12. Slodowski, O., Schöne, B., Böhm, J. & Otto, B. (1991) Carboxy-terminal truncated rhu-IFN-gamma with a substitution of Gln133 or Ser132 to leucine leads to higher biological activity than in the wild type, Eur. J. Biochem. 202, 1133-1140.
13. Higuchi, R., Krummel, B. & Saiki, R. K. (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions, Nucleic Acids Res. 16, 7351-7367.
14. Sanger, F., Nicklen, S. & Coulsen, A. R. (1977) DNA sequencing with chain-terminating inhibitors, Proc. Natl Acad. Sci. USA 74, 5463-5467.
15. Rubinstein, S., Familletti, P. C. & Pestka, S. (1981) Convenient assay for interferons, J. Virol. 37, 755-758.
16. Provencher, S. W. & Glöckner, J. (1981) Estimation of globular protein secondary structure from circular dichroism, Biochemistry 20, 33-37.
17. Fischer, T., Wiegmann, K., Böttinger, H., Morens, K., Burmester, G. & Pfizenmaier, K. (1990) Regulation of IFN-gamma-receptor expression in human monocytes by granulocyte-macrophage colony-stimulating factor, J. Immunol. 145, 2914-2919.
18. Fischer, T., Thoma, B., Scheurich, P. & Pfizenmaier, K. (1990) Glycosylation of the human interferon-gamma receptor. N-linked carbohydrates contribute to structural heterogeneity and are required for ligand binding, J. Biol. Chem. 265, 1710-1717.
19. Maiorov, V. N. & Crippen, G. M. (1994) Significance of root-mean-square deviation in comparing three-dimensional structures of globular proteins, J. Mol. Biol. 235, 625-634.
20. i) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction, Biochem. Pharmacol. 2, 3099-3108.
21. Sareneva, T., Pirhonen, J., Cantell, K. & Julkunen, I. (1995) N-glycosylation of human interferon-gamma: glycans at Asn-25 are critical for protease resistance, Biochem, J. 308, 9-14.
22. 125I-interferon-gamma to receptors on human cells, J. Biol. Chem. 260, 1191-1195.
23. 125I-labeled recombinant human gamma interferon to receptors on WISH cells, Proc. Natl Acad. Sci. USA 81, 5160-5164.
24. 125I-human interferon-gamma to high affinity receptors on human fibroblasts, J. Biol. Chem. 257, 11301-11304.
25. Slodowski, O. (1991) Carboxyterminal verkürzte Varianten des humanen Interferon-γ: Genkonstruktion, Expression in E. coli, Reinigung und Charakterisierung (PhD thesis), Universität Hannover, Germany.
26. Liang, S.-M., Lee, N., Zoon, K. C., Manischewitz, J. F., Chollet, A., Liang, C.-M. & Quinnan, G. V. (1988) Biological characterization of human Interleukin-2 mutant proteins, J. Biol. Chem. 263, 4768-4772.
27. Soh, J., Donelly, R. J., Kotenko, S., Mariano, T. M., Cook, R. J., Wang, N., Emanuel, S., Schwartz, B., Miki, T. & Petska, S. (1994) Identification and sequence of an accessory factor required for activation of the human interferon gamma receptor, Cell 76, 793-802.