Residues threonine 346 and leucine 352 are critical for the proper function of Bacillus kaustophilus leucine aminopeptidase

FEMS Microbiology Letters

Volumn 260, Issue 2, 2006, Pages 156-161

  Chi, Meng Chun ^a   Huang, Hsien Ben ^a   Liu, Jai Shin ^b   Wang, Wen Ching ^b   Liang, Wan Chi ^c   Lin, Long Liu ^c  

^a NATIONAL CHUNG CHENG UNIVERSITY   (Taiwan)

^b NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^c NATIONAL CHIAYI UNIVERSITY   (Taiwan)

Author keywords

Bacillus kaustophilus; Homology modeling; Leucine aminopeptidase; Site directed mutagenesis; Zinc coordination

Indexed keywords

CYTOSOL AMINOPEPTIDASE; LEUCINE; THREONINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS; BACILLUS KAUSTOPHILUS; BETA SHEET; ENZYME ACTIVITY; ENZYME ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DEGRADATION; RESIDUE ANALYSIS; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACILLUS; KINETICS; LEUCINE; LEUCYL AMINOPEPTIDASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP; THREONINE; ZINC;

ESCHERICHIA COLI; GEOBACILLUS KAUSTOPHILUS;

EID: 33745517267     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00309.x     Document Type: Article

References (20)

1. Altschul SF, Gish W, Miller W, Myers EW & Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
2. Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing ;the principle of protein-dye binding. Anal Biochem 72: 248-254.
3. Burley SK, David PR, Sweet RM, Taylor A & Lipscomb WN (1992) Structure determination and refinement of bovine lens leucine aminopeptidase and its complex with bestatin. J Mol Biol 224: 113-140.
4. 2+ of zinc metalloenzyme, amino acid composition, and sulfhydryl content. J Biol Chem 248: 294-304.
5. Chi MC, Chou WM, Hsu WH & Lin LL (2004) Identification of amino acid residues essential for the catalytic reaction of Bacillus kaustophilus leucine aminopeptidase. Biosci Biotechnol Biochem 68: 1784-1797.
6. Goldberg AL, Cascio P, Saric T & Rock KL (2002) The importance of the proteasome and subsequent proteolytic steps in the generation of antigenic peptides. Mol Immunol 39: 147-164.
7. Gonzales T & Robert-Baudouy J (1996) Bacterial aminopeptidases: properties and functions. FEMS Microbiol Lett 18: 319-344.
8. Kamphuis J, Meijer EM, Boesten WHJ, Broxterman QB, Kaptein B, Hermes HFM & Schoemaker HE (1992) Production of natural and synthetic L- and D-amino acids by aminopeptidases and amino amidases. Biocatalytic Production of Amino Acids and Derivatives (Rozzell JD & Wagner F, eds), pp. 178-206. Wiley, New York.
9. Kim H & Lipscomb WN (1993) Differentiation and identification of the two catalytic metal and binding sites in bovine lens leucine aminopeptidase by X-ray crystallography. Proc Natl Acad Sci USA 90: 5006-5010.
10. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.
11. Lin LL, Hsu WH, Wu CP, Chi MC, Chou WM & Hu HY (2004) A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223. Extremophiles 8: 79-87.
12. Melbye SW & Carpenter FH (1971) Leucine aminopeptidase (bovine lens): stability and size of subunits. J Biol Chem 246: 2459-2463.
13. Rao MB, Tanksale AM, Ghatge MS & Desphande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62: 597-635.
14. Sambrook J & Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. pp. 1.31-1.125. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
15. Sträter N & Lipscomb WN (1995) Two-metal ion mechanism of bovine lens leucine aminopeptidase: active site solvent structure and binding mode of L-leucinal, a gem-diolate transition state analogue, by X-ray crystallography. Biochemistry 34: 14792-14800.
16. Sträter N & Lipscomb WN (1998) Leucyl aminopeptidase (animal and plant). Handbook of Proteolytic Enzymes (Barrett AJ, Rawlings ND & Woessner JF, eds), pp. 1384-1389. Academic Press, New York.
17. Sträter N, Sun L, Kantrowitz ER & Lipscomb WN (1999) A bicarbonate ion as a general base in the mechanism of peptide hydrolysis by zinc leucine aminopeptidase. Proc Natl Acad Sci USA 96: 11151-11155.
18. Taylor A, Sanford D & Nowell T (1996) Structure and function of bovine lens aminopeptidase and comparison with homologous aminopeptidases. Aminopeptidases (Taylor A, ed), pp. 174-219. R.G. Landes Co., Austin, TX.
19. Terenius L, Sandin J & Sakurada T (2000) Nociceptin/orphanin FQ metabolism and bioactive metabolites. Peptides 21: 919-922.
20. Wallace AC, Laskowski RA & Thornton JM (1995) LIGPLOT: a program to generate schematic diagrams of protein - ligand interactions. Protein Eng 8: 127-134.