Site-directed mutagenesis of the conserved Ala348 and Gly350 residues at the putative active site of Bacillus kaustophilus leucine aminopeptidase

Biochimie

Volumn 90, Issue 5, 2008, Pages 811-819

  Chi, Meng Chun ^a   Liu, Jai Shin ^b   Wang, Wen Ching ^b   Lin, Long Liu ^c   Huang, Hsien Bin ^a  

^a NATIONAL CHUNG CHENG UNIVERSITY   (Taiwan)

^b NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^c NATIONAL CHIAYI UNIVERSITY   (Taiwan)

Author keywords

Bacillus kaustophilus; Circular dichroism; Leucine aminopeptidase; Protein modeling; Site directed mutagenesis; Tryptophan emission fluorescence

Indexed keywords

CYTOSOL AMINOPEPTIDASE; MUTANT PROTEIN; ZINC;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACILLUS KAUSTOPHILUS; BACTERIAL GROWTH; CIRCULAR DICHROISM; COMPUTER MODEL; ENZYME ACTIVE SITE; ENZYME ASSAY; FLUORESCENCE SPECTROSCOPY; GEL ELECTROPHORESIS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; WILD TYPE;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; BINDING SITES; DNA PRIMERS; LEUCYL AMINOPEPTIDASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, FLUORESCENCE;

GEOBACILLUS KAUSTOPHILUS;

EID: 42649132153     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2007.11.011     Document Type: Article

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