Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies

Protein Expression and Purification

Volumn 47, Issue 2, 2006, Pages 498-505

  Li, Yifeng ^a   Li, Xia ^a   Wang, Guangshun ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Antimicrobial peptide; Chemical cleavage; Escherichia coli; Formic acid; Isotope labeling; LL 37; MUC1 cytoplasmic tail; NMR; P LL37

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; CAP18 LIPOPOLYSACCHARIDE BINDING PROTEIN; CAP18 LIPOPOLYSACCHARIDE-BINDING PROTEIN; HYBRID PROTEIN; MUC1 PROTEIN, HUMAN; MUCIN; NITROGEN; TUMOR ANTIGEN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE;

ANTIGENS, NEOPLASM; ANTIMICROBIAL CATIONIC PEPTIDES; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; ISOTOPE LABELING; MUCINS; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33646726320     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.10.022     Document Type: Article

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