High level expression and purification of antimicrobial human cathelicidin LL-37 in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 88, Issue 1, 2010, Pages 167-175

  Krahulec, Ján ^a,b   Hyršová, Marcela ^a   Pepeliaev, Stanislav ^a   Jílková, Jana ^a   Černý, Zbyněk ^a   MacHálková, Jana ^a  

^a Comenius University in Bratislava ^*  (Czech Republic)

^b Faculty of Natural Sciences   (Slovakia)

Author keywords

Antimicrobial peptide; Cathelicidin; Human; Recombinant

Indexed keywords

ANTI-MICROBIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; CANDIDA ALBICANS; CATHELICIDIN; CATIONIC PEPTIDES; E. COLI; ENTEROCOCCUS FAECALIS; EXPRESSION SYSTEM; EXTRACELLULAR SPACE; FUSION PROTEINS; GALACTOSIDASES; HIGH LEVEL EXPRESSION; HUMAN; IN-CELL; RECOMBINANT; REGULATION MECHANISMS; SELECTION PRESSURES; STAPHYLOCOCCUS AUREUS;

BIOINFORMATICS; CELL CULTURE; CELL MEMBRANES; ESCHERICHIA COLI; PEPTIDES; POLYPEPTIDES; SCANNING ELECTRON MICROSCOPY;

MICROORGANISMS;

ARABINOSE; BACTERIAL PROTEIN; BETA GALACTOSIDASE; CATHELICIDIN ANTIMICROBIAL PEPTIDE LL 37; PARB PROTEIN; UNCLASSIFIED DRUG;

ANTIBIOTICS; ANTIMICROBIAL ACTIVITY; COLIFORM BACTERIUM; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; INHIBITION; MEMBRANE; PEPTIDE; PLASMID; PROTEIN; RECOMBINATION; SCANNING ELECTRON MICROSCOPY;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL STRAIN; CANDIDA ALBICANS; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; EXTRACELLULAR SPACE; GENE EXPRESSION SYSTEM; GENE LOCUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REGULATORY MECHANISM; SCANNING ELECTRON MICROSCOPY; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS;

ANTIMICROBIAL CATIONIC PEPTIDES; BACTERIOPHAGE T7; CANDIDA ALBICANS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA-DIRECTED RNA POLYMERASES; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; MICROBIAL SENSITIVITY TESTS; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS; VIRAL PROTEINS;

CANDIDA ALBICANS; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 77955849956     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2736-7     Document Type: Article

References (32)

1. B Agerberth J Charo J Werr B Olson F Idali L Lindbom R Kiessling H Jornvall H Wigzell GH Gudmundsson 2000 The human antimicrobial and chemotactic peptides LL-37 and alpha-defensins are expressed by specific lymphocyte populations Blood 96 3086 3093 1:CAS:528:DC%2BD3cXnslGnt74%3D
2. R Bals JM Wilson 2003 Cathelicidins-a family of multifunctional antimicrobial peptides Cell Mol Life Sci 60 711 720 10.1007/s00018-003-2186-9 1:CAS:528:DC%2BD3sXktlyjurk%3D (Pubitemid 36570762)
3. DM Bowdish DJ Davidson RE Hancock 2006 Immunomodulatory properties of defensins and cathelicidins Curr Top Microbiol Immunol 306 27 66 10.1007/3-540-29916-5-2 1:CAS:528:DC%2BD28Xos1ymtb8%3D
4. RA Dorschner VK Pestonjamasp S Tamakuwala T Ohtake J Rudisill V Nizet B Agerberth GH Gudmundsson RL Gallo 2001 Cutaneous injury induces the release of cathelicidin anti-microbial peptides active against group A Streptococcus J Invest Dermatol 117 91 97 10.1046/j.1523-1747.2001.01340.x 1:CAS:528: DC%2BD3MXlsFagt7k%3D (Pubitemid 32652444)
5. T Ganz 2004 Defensins: antimicrobial peptides of vertebrates C R Biol 327 539 549 10.1016/j.crvi.2003.12.007 1:CAS:528:DC%2BD2cXls1Wlt7s%3D (Pubitemid 38979504)
6. BAD promoter J Bacteriol 177 4121 4130 1:CAS:528:DyaK2MXmvV2gtL8%3D
7. IP Hong SJ Lee YS Kim SG Choi 2007 Recombinant expression of the human cathelicidin (hCAP18/LL-37) in Pichia pastoris Biotechnol Lett 29 73 78 10.1007/s10529-006-9202-8 1:CAS:528:DC%2BD28Xht12gs7fL (Pubitemid 44950160)
8. U Horn W Strittmatter A Krebber U Knüpfer R Wenderoth K Müller S Matzku A Plückthun D Riesenberg 1996 High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions Appl Microbiol Biotechnol 46 524 532 10.1007/s002530050855 1:CAS:528: DyaK2sXht1Sqtb8%3D (Pubitemid 26426411)
9. E Isogai H Isogai K Matuo K Hirose Y Kowashi K Okumuara M Hirata 2003 Sensitivity of genera Porphyromonas and Prevotella to the bactericidal action of C-terminal domain of human CAP18 and its analogues Oral Microbiol Immunol 18 329 332 10.1034/j.1399-302X.2003.00083.x 1:CAS:528:DC%2BD3sXnvF2ltbc%3D (Pubitemid 37087268)
10. R Koczulla G von Degenfeld C Kupatt F Krotz S Zahler T Gloe K Issbrucker P Unterberger M Zaiou A Karl P Raake A Pfosser P Boekstegers U Welsch PS Hiemstra C Vogelmeier RL Gallo M Clauss R Bals 2003 An angiogenic role for the human peptide antibiotic LL-37/hCAP-18 J Clin Invest 111 1665 1672 1:CAS:528:DC%2BD3sXksVKrsL4%3D
11. RI Lehrer T Granz 1998 Antimicrobial peptides in mammalian and insect host defence Curr Opin Immunol 11 23 27 10.1016/S0952-7915(99)80005-3
12. RI Lehrer A Barton T Ganz 1988 Concurrent assessment of inner and outer membrane permeabilization and bacteriolysis in E. coli by multiple-wavelength spectrophotometry J Immunol Methods 108 153 158 10.1016/0022-1759(88)90414-0 1:CAS:528:DyaL1cXktlClu7o%3D
13. RI Lehrer M Rosenman SSSL Harwing R Jackson 1991 Ultrasensitive assays for endogenous antimicrobial polypeptides J Immunol Methods 137 167 173 10.1016/0022-1759(91)90021-7 1:CAS:528:DyaK3MXisVGmtrc%3D
14. Y Li X Li G Wang 2006 Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies Protein Expr Purif 47 498 505 10.1016/j.pep.2005.10.022 1:CAS:528:DC%2BD28XltVKit78%3D (Pubitemid 43744452)
15. Y Li X Li H Li O Lockridge G Wang 2007 A novel method for purifying recombinant human host defense cathelicidin LL-37 by utilizing its inherent property of aggregation Protein Expr Purif 54 157 165 10.1016/j.pep.2007.02.003 1:CAS:528:DC%2BD2sXkslCntL4%3D (Pubitemid 46636313)
16. E Maguin H Prevost SD Ehrlich A Gruss 1996 Efficient insertion mutagenesis in Lactococci and other Gram-positive bacteria J Bacteriol 178 931 935 1:CAS:528:DyaK28XnsFWhug%3D%3D
17. JY Moon KA Henzler-Wildman A Ramamoorthy 2006 Expression and purification of a recombinant LL-37 from Escherichia coli Biochim Biophys Acta 1758 1351 1358 10.1016/j.bbamem.2006.02.003 1:CAS:528:DC%2BD28XhtVanu7nO (Pubitemid 44436070)
18. PY Ong T Ohtake C Brandt I Strickland M Boguniewicz T Ganz RL Gallo DY Leung 2002 Endogenous antimicrobial peptides and skin infection in atopic dermatitis N Engl J Med 347 1151 1160 10.1056/NEJMoa021481 1:CAS:528: DC%2BD38XnslShsb0%3D
19. Z Oren JC Lerman GH Gudmundson B Agerberth Y Shai 1999 Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molar basis for its non-cell-selective activity Biochem J 341 501 513 10.1042/0264-6021:3410501 1:CAS:528:DyaK1MXltlarurk%3D (Pubitemid 29389178)
20. S Schaller-Bals A Schulze R Bals 2002 Increased levels of antimicrobial peptides in tracheal aspirates of newborn infants during infection Am J Respir Crit Care Med 165 992 995
21. B Schittek R Hipfel B Sauer J Bauer H Kalbacher S Stevanovic M Schirle K Schroeder N Blin F Meier G Rasner C Garbe 2001 Dermcidin: a novel human antibiotic peptide secreted by sweat glands Nat Immunol 2 1133 1137 10.1038/ni732 1:CAS:528:DC%2BD3MXptVaitrc%3D (Pubitemid 33130147)
22. R Shaykhiev C Beisswenger K Kandler J Sneske A Puchner T Damm J Behr R Bals 2005 Human endogenous antibiotic LL-37 stimulates airway epithelial cell proliferation and wound closure Am J Physiol Lung Cell Mol Physiol 289 L842 L848 10.1152/ajplung.00286.2004 1:CAS:528:DC%2BD2MXht1Cnt7vM (Pubitemid 41504416)
23. V Smeianov K Scott G Reid 2000 Activity of cecropin P1 and FA-LL-37 against urogenital microflora Microbes Infect 2 773 777 10.1016/S1286-4579(00) 90359-9 1:CAS:528:DC%2BD3cXlvFelsrs%3D (Pubitemid 30451218)
24. OE Sorensen P Follin AH Johnsen J Calafat GS Tjabringa PS Hiemstra N Borregaard 2001 Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3 Blood 97 3951 3959 10.1182/blood.V97.12.3951 1:CAS:528:DC%2BD3MXksVOqtrw%3D
25. Stahle-Backdahl M, Heilborn J, Carlsson A, Bogentoft C (2008) Use of the cathelicidin LL-37 and derivatives thereof for wound healing, US Patent 7452864
26. S Tabor CC Richardson 1984 A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proc Natl Acad Sci USA 82 1074 1078 10.1073/pnas.82.4.1074
27. D Tanaka KT Miyasaki RI Lehrer 2000 Sensitivity of Actinobacillus actinomycetemcomitans and Capnocytophaga spp. to the bacterial action of LL-37: a cathelicidin found in human leucocytes and epithelium Oral Microbiol Immunol 15 226 231 10.1034/j.1399-302x.2000.150403.x 1:CAS:528:DC%2BD3cXlsFSrsL0%3D
28. GS Tjabringa DK Ninaber JW Drijfhout KF Rabe PS Hiemstra 2006 Human cathelicidin LL-37 is a chemoattractant for eosinophils and neutrophils that acts via formyl-peptide receptors Int Arch Allergy Immunol 140 103 112 10.1159/000092305 1:CAS:528:DC%2BD28Xktlyiur0%3D (Pubitemid 43725804)
29. J Turner Y Cho NN Dinh AJ Waring 1998 Activities of LL-37, a cathelin-associated antimicrobial peptide of human neutrophils Antimicrob Agents Chemother 42 2206 2214 1:CAS:528:DyaK1cXmtVWqs78%3D
30. C Yanisch-Perron J Vieira J Messing 1985 Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors Gene 33 103 119 10.1016/0378-1119(85)90120-9 1:CAS:528:DyaL2MXktVWgtL0%3D (Pubitemid 15122816)
31. M Zanetti 2004 Cathelicidins, multifunctional peptides of the innate immunity J Leukoc Biol 75 39 48 10.1189/jlb.0403147 (Pubitemid 38166778)
32. M Zanetti 2005 The role of cathelicidins in the innate host defenses of mammals Curr Issues Mol Biol 7 179 196 1:CAS:528:DC%2BD2MXotFarurY%3D