A novel method for purifying recombinant human host defense cathelicidin LL-37 by utilizing its inherent property of aggregation

Protein Expression and Purification

Volumn 54, Issue 1, 2007, Pages 157-165

  Li, Yifeng ^a   Li, Xia ^a   Li, He ^a   Lockridge, Oksana ^a   Wang, Guangshun ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Antimicrobial peptides; Cathelicidins; Chemical cleavage; Enzyme cleavage; Escherichia coli; FPLC; HPLC; LL 37; NMR; Protein aggregation

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; CAP18 LIPOPOLYSACCHARIDE BINDING PROTEIN; CAP18 LIPOPOLYSACCHARIDE-BINDING PROTEIN; FORMIC ACID; FORMIC ACID DERIVATIVE; NITROGEN; RECOMBINANT PROTEIN; THROMBIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; NUCLEAR MAGNETIC RESONANCE;

ANTIMICROBIAL CATIONIC PEPTIDES; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; FORMIC ACIDS; HUMANS; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; RECOMBINANT PROTEINS; THROMBIN;

ESCHERICHIA COLI;

EID: 34247370223     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.02.003     Document Type: Article

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