Directed evolution improves the fibrinolytic activity of nattokinase from Bacillus natto

FEMS Microbiology Letters

Volumn 325, Issue 2, 2011, Pages 155-161

  Yongjun, Cai ^a   Wei, Bao ^a   Shujun, Jiang ^a   Meizhi, Weng ^a   Yan, Jia ^a   Yan, Yin ^a   Zhongliang, Zheng ^a   Goulin, Zou ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Directed evolution; DNA family shuffling; Fibrinolytic activity; Nattokinase; Subtilisin

Indexed keywords

NATTOKINASE; SUBTILISIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; APRN GENE; ARTICLE; BACILLUS AMYLOLIQUEFACIENS; BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM MUTANT; CATALYSIS; CONFORMATION; CONTROLLED STUDY; DIRECTED EVOLUTION; ENZYME ACTIVITY; ENZYME ANALYSIS; FIBRINOLYSIS; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOLECULAR MODEL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; WILD TYPE;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; BINDING SITES; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; FIBRINOLYSIS; GENE LIBRARY; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; OLIGOPEPTIDES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBTILISINS;

EID: 81855185465     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2011.02423.x     Document Type: Article

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