Biochemical and molecular characterization of a detergent-stable serine alkaline protease from Bacillus pumilus CBS with high catalytic efficiency

Biochimie

Volumn 90, Issue 9, 2008, Pages 1291-1305

  Jaouadi, Bassem ^a   Ellouz Chaabouni, Semia ^b   Rhimi, Moez ^a   Bejar, Samir ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

^b UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Bacillus pumilus CBS; Catalytic efficiency; MALDI TOF MS; NH2 terminal sequence; SAPB

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); ALKALINE PROTEINASE; BENZAMIDE; CASEIN; DETERGENT; DODECYL SULFATE SODIUM; DYFLOS; EDETIC ACID; GELATIN; HYDROGEN PEROXIDE; IODOACETAMIDE; LEUPEPTIN; METAL ION; OXIDIZING AGENT; PEPSTATIN; POLYOL; POLYSORBATE 20; POLYSORBATE 80; RECOMBINANT ENZYME; SERINE ALKALINE PROTEASE; SODIUM CHLORIDE; SUBTILISIN; TOSYLLYSYL CHLOROMETHYL KETONE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; TRITON X 100; UREA;

ARTICLE; BACILLUS PUMILUS; ELECTROPHORESIS; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; SEQUENCE ANALYSIS; ZYMOGRAPHY;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; BASE SEQUENCE; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DETERGENTS; ENDOPEPTIDASES; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; MOLECULAR STRUCTURE; OXIDANTS; PROTEASE INHIBITORS; PROTEIN DENATURATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACILLUS PUMILUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 47749138577     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2008.03.004     Document Type: Article

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