Combinatorial engineering to enhance amylosucrase performance: Construction, selection, and screening of variant libraries for increased activity

FEBS Letters

Volumn 560, Issue 1-3, 2004, Pages 91-97

  Van Der Veen, Bart A ^a   Potocki Véronèse, Gabrielle ^a   Albenne, Cécile ^a   Joucla, Gilles ^a   Monsan, Pierre ^a   Remaud Simeon, Magali ^a  

^a UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

Author keywords

Amylosucrase; Efficiency screening; Error prone polymerase chain reaction; Gene shuffling; Positive selection; Zero background expression cloning

Indexed keywords

AMINO ACID; AMYLOSUCRASE; CARBON; ENZYME VARIANT; FRUCTOSE; GLUCOSE; GLYCOSIDASE; IODINE; POLYMER; POLYSACCHARIDE; REDUCING AGENT; SALICYLIC ACID; SUGAR; TAQ POLYMERASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CATALYSIS; COMPARATIVE STUDY; DNA LIBRARY; ENZYME ACTIVITY; ENZYME STABILITY; GENE ACTIVITY; GENE LIBRARY; GENE SEQUENCE; GENE TECHNOLOGY; GENETIC ENGINEERING; GENETIC SELECTION; GLYCOSYLATION; HYDROLYSIS; ISOMER; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STAINING; SYNTHESIS; WILD TYPE;

EID: 1342286824     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(04)00077-8     Document Type: Article

References (24)

1. Buttcher V., Welsh T., Willmitzer L., Kossmann J. J. Bacteriol. 179:1997;3324-3330.
2. Potocki de Montalk G., Remaud-Simeon M., Willemot R.M., Planchot V., Monsan P. J. Bacteriol. 181:1999;375-381.
3. Potocki de Montalk G., Remaud-Simeon M., Willemot R.M., Sarçabal P., Planchot V., Monsan P. FEBS Lett. 471:2000;219-223.
4. Coutinho, P.M. and Henrissat, B. (1999) Carbohydrate-Active Enzyme server at URL: http://afmb.cnrs-mrs.fr/~cazy/CAZY/index.html.
5. Preiss J., Ozbun J.L., Hawker J.S., Greenberg E., Lammel C. Ann. NY Acad. Sci. 210:1973;265-278.
6. Hehre E.J., Hamilton D.M., Carlson A.S. J. Biol. Chem. 177:1949;267-279.
7. Skov L.K., Mirza O., Henriksen A., Potocki de Montalk G., Remaud-Simeon M., Sarçabal P., Willemot R.M., Monsan P., Gajhede M. J. Biol. Chem. 276:2001;25273-25278.
8. Potocki de Montalk G., Remaud-Simeon M., Willemot Monsan P. FEMS Microbiol. Lett. 186:2000;103-108.
9. Albenne C., Skov L.K., Mirza O., Gajhede M., Potocki-Veronese G., Monsan P., Remaud-Simeon M. FEBS Lett. 527:2002;67-70.
10. Mirza O., Skov L.K., Remaud-Simeon M., Potocki de Montalk G., Albenne C., Monsan P., Gajhede M. Biochemistry. 40:2001;9032-9039.
11. Skov L.K., Mirza O., Sprogoe D., Dar L., Remaud-Simeon M., Albenne C., Monsan P., Gajhede M. J. Biol. Chem. 277:2002;47741-47747.
12. Sarçabal P., Remaud-Simeon M., Willemot R.M., Potocki de Montalk G., Svensson B., Monsan P. FEBS Lett. 474:2000;33-37.
13. Albenne C., Potocki-Veronese G., Monsan P., Skov L.K., Mirza O., Gajhede M., Remaud-Simeon M. Biologia (Bratislava). 57:2002;119-128.
14. Albenne C., Skov L.K., Mirza O., Gajhede M., Feller G., D'Amico S., Andre G., Potocki-Véronèse G., van der Veen B.A., Monsan P., Remaud-Simeon M. J. Biol. Chem. 279:2004;726-734.
15. Lehman M., Wijss M. Curr. Opin. Biotechnol. 12:2001;371-375.
16. Cadwell R.C., Joyce G.F. PCR Methods Appl. 2:1992;28-33.
17. Stemmer W.P. Proc. Natl. Acad. Sci. USA. 91:1994;10747-10751.
18. Sanger F., Nicklen S., Coulson A.R. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
19. van de Guchte M., Kok J., Venema G. Mol. Gen. Genet. 227:1991;65-71.
20. Zhao H., Arnold F.H. Nucleic Acids Res. 25:1997;1307-1308.
21. Kikuchi M., Ohnishi K., Harayama S. Gene. 236:1999;159-167.
22. Sumner J.B., Howell S.F. J. Biol. Chem. 108:1935;51-54.
23. Banks W., Greenwood C.T., Kahn K.M. Carbohydr. Res. 17:1971;25-33.
24. Bradford M.M. Anal. Biochem. 72:1976;248-254.