Enhancement of the thermostability and the catalytic efficiency of Bacillus pumilus CBS protease by site-directed mutagenesis

Biochimie

Volumn 92, Issue 4, 2010, Pages 360-369

  Jaouadi, Bassem ^a   Aghajari, Nushin ^b   Haser, Richard ^b   Bejar, Samir ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

^b INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

Author keywords

Bacillus pumilus; Homology modeling; Protease; Sequence alignments; Site directed mutagenesis

Indexed keywords

ALKALINE PROTEINASE; SUBTILISIN;

ARTICLE; BACILLUS PUMILUS; BACTERIAL STRAIN; BIOCOMPATIBILITY; CONTROLLED STUDY; ENZYME ACTIVITY; GENE MUTATION; KINETICS; MICHAELIS MENTEN KINETICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; TEMPERATURE; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

AMINO ACID SEQUENCE; BACILLUS; ENZYME STABILITY; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ALIGNMENT; SUBTILISINS;

BACILLUS PUMILUS;

EID: 77649182694     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2010.01.008     Document Type: Article

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