Development of a specific ELISA to measure BACE1 levels in human tissues

Journal of Neuroscience Methods

Volumn 202, Issue 1, 2011, Pages 70-76

  Gonzales, Amanda ^a   Decourt, Boris ^a   Walker, Aaron ^a   Condjella, Rachel ^a   Nural, Hikmet ^a   Sabbagh, Marwan N ^a  

^a SUN HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

BACE1; Brain; ELISA; Human; Plasma; Platelets

Indexed keywords

MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 195101; MONOCLONAL ANTIBODY 195102; MONOCLONAL ANTIBODY 28051; MONOCLONAL ANTIBODY AF931; MONOCLONAL ANTIBODY B0681; MONOCLONAL ANTIBODY B0806; MONOCLONAL ANTIBODY MAB 931; MONOCLONAL ANTIBODY MAB 9311; MONOCLONAL ANTIBODY MAB5308; MONOCLONAL ANTIBODY PA1 757; RECOMBINANT BETA SECRETASE 1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ALZHEIMER DISEASE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY DETECTION; ANTIBODY SCREENING; ANTIBODY SPECIFICITY; ARTICLE; BRAIN TISSUE; CONTROLLED STUDY; DILUTION; ENZYME ACTIVE SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN TISSUE; INCUBATION TEMPERATURE; MALE; MOUSE; NONHUMAN; PLASMA; PRIORITY JOURNAL; REPRODUCIBILITY; SIGNAL NOISE RATIO; THROMBOCYTE; WESTERN BLOTTING;

ALZHEIMER DISEASE; AMYLOID PRECURSOR PROTEIN SECRETASES; ANIMALS; ANTIBODIES; ANTIBODY SPECIFICITY; ASPARTIC ACID ENDOPEPTIDASES; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MICE; MICE, INBRED C57BL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 80053570056     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.08.030     Document Type: Article

References (34)

1. Ahmed R.R., Holler C.J., Webb R.L., Li F., Beckett T.L., Murphy M.P. BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J Neurochem 2010, 112:1045-1053.
2. Beach T.G., Sue L.I., Walker D.G., Roher A.E., Lue L., Vedders L., et al. The Sun Health Research Institute Brain Donation Program: description and experience, 1987-2007. Cell Tissue Bank 2008, 9:229-245.
3. Bush A.I., Martins R.N., Rumble B., Moir R., Fuller S., Milward E., et al. The amyloid precursor protein of Alzheimer's disease is released by human platelets. J Biol Chem 1990, 265:15977-15983.
4. Cole S.L., Vassar R. BACE1 structure and function in health and Alzheimer's disease. Curr Alzheimer Res 2008, 5:100-120.
5. Creemers J.W., Ines Dominguez D., Plets E., Serneels L., Taylor N.A., Multhaup G., et al. Processing of beta-secretase by furin and other members of the proprotein convertase family. J Biol Chem 2001, 276:4211-4217.
6. Decourt B., Sabbagh M.N. BACE1 as a potential biomarker for Alzheimer's Disease. J Alzheimers Dis 2011, 2(Suppl.):53-59.
7. Ermolieff J., Loy J.A., Koelsch G., Tang J. Proteolytic activation of recombinant pro-memapsin 2 (pro-beta-secretase) studied with new fluorogenic substrates. Biochemistry 2000, 39:12450-12456.
8. Fukumoto H., Cheung B.S., Hyman B.T., Irizarry M.C. Beta-secretase protein and activity are increased in the neocortex in Alzheimer disease. Arch Neurol 2002, 59:1381-1389.
9. Gruninger-Leitch F., Schlatter D., Kung E., Nelbock P., Dobeli H. Substrate and inhibitor profile of BACE (beta-secretase) and comparison with other mammalian aspartic proteases. J Biol Chem 2002, 277:4687-4693.
10. Hayley M., Perspicace S., Schulthess T., Seelig J. Calcium enhances the proteolytic activity of BACE1: an in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta 2009, 1788:1933-1938.
11. Hoffmeister A., Dietz G., Zeitschel U., Mossner J., Rossner S., Stahl T. BACE1 is a newly discovered protein secreted by the pancreas which cleaves enteropeptidase in vitro. JOP 2009, 10:501-506.
12. Holsinger R.M., Lee J.S., Boyd A., Masters C.L., Collins S.J. CSF BACE1 activity is increased in CJD and Alzheimer disease versus [corrected] other dementias. Neurology 2006, 67:710-712.
13. Holsinger R.M., McLean C.A., Beyreuther K., Masters C.L., Evin G. Increased expression of the amyloid precursor beta-secretase in Alzheimer's disease. Ann Neurol 2002, 51:783-786.
14. Hu X., He W., Diaconu C., Tang X., Kidd G.J., Macklin W.B., et al. Genetic deletion of BACE1 in mice affects remyelination of sciatic nerves. FASEB J 2008, 22:2970-2980.
15. Hussain I., Powell D., Howlett D.R., Tew D.G., Meek T.D., Chapman C., et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci 1999, 14:419-427.
16. Kalvodova L., Kahya N., Schwille P., Ehehalt R., Verkade P., Drechsel D., et al. Lipids as modulators of proteolytic activity of BACE: involvement of cholesterol, glycosphingolipids, and anionic phospholipids in vitro. J Biol Chem 2005, 280:36815-36823.
17. Kienlen-Campard P., Feyt C., Huysseune S., de Diesbach P., N'Kuli F., Courtoy P.J., et al. Lactacystin decreases amyloid-beta peptide production by inhibiting beta-secretase activity. J Neurosci Res 2006, 84:1311-1322.
18. Kitazume S., Tachida Y., Oka R., Shirotani K., Saido T.C., Hashimoto Y. Alzheimer's beta-secretase, beta-site amyloid precursor protein-cleaving enzyme, is responsible for cleavage secretion of a Golgi-resident sialyltransferase. Proc Natl Acad Sci USA 2001, 98:13554-13559.
19. Kume H., Konishi Y., Murayama K.S., Kametani F., Araki W. Expression of reticulon 3 in Alzheimer's disease brain. Neuropathol Appl Neurobiol 2009, 35:178-188.
20. Li Q., Sudhof T.C. Cleavage of amyloid-beta precursor protein and amyloid-beta precursor-like protein by BACE 1. J Biol Chem 2004, 279:10542-10550.
21. Lichtenthaler S.F., Dominguez D.I., Westmeyer G.G., Reiss K., Haass C., Saftig P., et al. The cell adhesion protein P-selectin glycoprotein ligand-1 is a substrate for the aspartyl protease BACE1. J Biol Chem 2003, 278:48713-48719.
22. McKhann G., Drachman D., Folstein M., Katzman R., Price D., Stadlan E.M. Clinical diagnosis of Alzheimer's disease: report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer's Disease. Neurology 1984, 34:939-944.
23. Murayama K.S., Kametani F., Araki W. Extracellular release of BACE1 holoproteins from human neuronal cells. Biochem Biophys Res Commun 2005, 338:800-807.
24. Sinha S., Anderson J.P., Barbour R., Basi G.S., Caccavello R., Davis D., et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999, 402:537-540.
25. Vassar R., Bennett B.D., Babu-Khan S., Kahn S., Mendiaz E.A., Denis P., et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999, 286:735-741.
26. Vassar R., Kovacs D.M., Yan R., Wong P.C. The beta-secretase enzyme BACE in health and Alzheimer's disease: regulation, cell biology, function, and therapeutic potential. J Neurosci 2009, 29:12787-12794.
27. Verheijen J.H., Huisman L.G., van Lent N., Neumann U., Paganetti P., Hack C.E., et al. Detection of a soluble form of BACE-1 in human cerebrospinal fluid by a sensitive activity assay. Clin Chem 2006, 52:1168-1174.
28. von Arnim C.A., Kinoshita A., Peltan I.D., Tangredi M.M., Herl L., Lee B.M., et al. The low density lipoprotein receptor-related protein (LRP) is a novel beta-secretase (BACE1) substrate. J Biol Chem 2005, 280:17777-17785.
29. Wong H.K., Sakurai T., Oyama F., Kaneko K., Wada K., Miyazaki H., et al. Beta Subunits of voltage-gated sodium channels are novel substrates of beta-site amyloid precursor protein-cleaving enzyme (BACE1) and gamma-secretase. J Biol Chem 2005, 280:23009-23017.
30. Yan R., Bienkowski M.J., Shuck M.E., Miao H., Tory M.C., Pauley A.M., et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999, 402:533-537.
31. Yang L.B., Lindholm K., Yan R., Citron M., Xia W., Yang X.L., et al. Elevated beta-secretase expression and enzymatic activity detected in sporadic Alzheimer disease. Nat Med 2003, 9:3-4.
32. Zetterberg H., Andreasson U., Hansson O., Wu G., Sankaranarayanan S., Andersson M.E., et al. Elevated cerebrospinal fluid BACE1 activity in incipient Alzheimer disease. Arch Neurol 2008, 65:1102-1107.
33. Zhao J., Fu Y., Yasvoina M., Shao P., Hitt B., O'Connor T., et al. Beta-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer's disease pathogenesis. J Neurosci 2007, 27:3639-3649.
34. Zhong Z., Ewers M., Teipel S., Burger K., Wallin A., Blennow K., et al. Levels of beta-secretase (BACE1) in cerebrospinal fluid as a predictor of risk in mild cognitive impairment. Arch Gen Psychiatry 2007, 64:718-726.