Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies

Protein Expression and Purification

Volumn 81, Issue 1, 2012, Pages 11-17

  Chen, Wen ^a   Gamache, Eric ^a   Richardson, Danielle ^a   Du, Zhenming ^a   Wang, Chunyu ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Secretase; Alzheimer's disease; Amyloid precursor protein (APP); APPTM; HSQC; Membrane protein; Micelle; Transmembrane domain

Indexed keywords

AMYLOID PRECURSOR PROTEIN; APP PROTEIN, HUMAN; DODECYLPHOSPHORYLCHOLINE; DRUG DERIVATIVE; NITROGEN; PHOSPHORYLCHOLINE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MICELLE; NUCLEAR MAGNETIC RESONANCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; REPRODUCIBILITY;

AMYLOID BETA-PROTEIN PRECURSOR; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MASS SPECTROMETRY; MICELLES; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHORYLCHOLINE; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS;

TOBACCO ETCH VIRUS;

EID: 80053454072     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.08.006     Document Type: Article

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