Phosphoproteomic analysis of Salmonella-infected cells identifies key kinase regulators and SopB-dependent host phosphorylation events

Science Signaling

Volumn 4, Issue 191, 2011, Pages

  Rogers, Lindsay D ^a   Brown, Nat F ^b   Fang, Yuan ^a   Pelech, Steven ^a,c   Foster, Leonard J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c Kinexus Bioinformatics Corporation   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH FACTOR; PROTEIN KINASE B; PROTEIN KINASE C; PROTEIN KINASE PIM 1; PROTEIN SERINE THREONINE KINASE; PROTEIN SOPB; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; PROTEIN KINASE; SOPB PROTEIN, SALMONELLA;

ARTICLE; BACTERIAL GROWTH; CONTROLLED STUDY; GASTROENTERITIS; HOST; HOST CELL; HUMAN; HUMAN CELL; IMMUNE RESPONSE; INFORMATION SCIENCE; INTRACELLULAR SIGNALING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEOMICS; QUANTITATIVE ANALYSIS; SALMONELLA ENTERICA; SALMONELLOSIS; TYPE III SECRETION SYSTEM; VIRULENCE; ENZYMOLOGY; HELA CELL; HOST PATHOGEN INTERACTION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PHOSPHORYLATION; PHYSIOLOGY; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); SALMONELLA; SALMONELLA ENTERICA;

BACTERIAL PROTEINS; HELA CELLS; HOST-PATHOGEN INTERACTIONS; HUMANS; PHOSPHORYLATION; PROTEIN KINASES; PROTEOMICS; SALMONELLA ENTERICA; SALMONELLA INFECTIONS; SIGNAL TRANSDUCTION;

EID: 80053111807     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.2001668     Document Type: Article

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81. Acknowledgments: We thank B. Finlay for the ΔsopB strain and for reading the manuscript, as well as other members of our group for discussions, advice, and technical support, particularly H. Zhang and J. Safaei for their contributions. Funding: A Canadian Institutes of Health Research (CIHR) operating grant (MOP-77688) to L.J.F. supported this work. Infrastructure used in this work was supported by the Canada Foundation for Innovation, the British Columbia (BC) Knowledge Development Fund, and the BC Proteomics Network. L.J.F. is the Canada Research Chair in Quantitative Proteomics. L.D.R. is supported by a CIHR CGS-D award and a Michael Smith Foundation for Health Research Trainee Award. Author contributions: L.D.R. and L.J.F. designed the phosphoproteomics experiments. L.D.R. conducted the phosphoproteomics experiments and analyzed the data, with assistance from Y.F. N.F.B. and L.D.R. conducted the IL-8 ELISA experiments, and N.F.B. constructed Fig. 6. Kinase predictions were done by S.P. L.D.R. wrote the initial draft of the manuscript. Competing interests: S.P. owns Kinexus Bioinformatics, a company involved in analyzing phosphorylation. Data availability: Raw MS/MS spectra acquired in this study are available at the PRoteomics IDEntification (PRIDE) database (http://www.ebi.ac.uk/pride/) under experiment accession numbers 18477 and 18485.