Simple defined autoinduction medium for high-level recombinant protein production using T7-based Escherichia coli expression systems

Applied Microbiology and Biotechnology

Volumn 91, Issue 4, 2011, Pages 1203-1213

  Li, Zhaopeng ^a,b   Kessler, Wolfgang ^a   Van Den Heuvel, Joop ^a   Rinas, Ursula ^a,b  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

Autoinduction; Defined medium; Escherichia coli; Recombinant protein production

Indexed keywords

96-WELL MICROTITER PLATE; AUTOINDUCTION; AVERAGE YIELD; CARBON SUBSTRATES; DEFINED MEDIUM; DIAUXIC GROWTH; E. COLI; ESCHERICHIA COLI EXPRESSION SYSTEMS; EXPRESSION SYSTEM; LAC OPERON; LURIA-BERTANI BROTHS; NITROGEN SOURCES; PROTEIN CONCENTRATIONS; PROTEIN PRODUCTION; RECOMBINANT PROTEIN PRODUCTIONS; REGULATORY ELEMENTS; SHAKE FLASKS; TARGET PROTEINS; TEST TUBE;

AMINO ACIDS; BOTTLES; ESCHERICHIA COLI; GLUCOSE; GLYCEROL; SUBSTRATES;

PROTEINS;

GLUCOSE; GLYCEROL; LACTOSE; NITROGEN; RECOMBINANT PROTEIN;

AMINO ACID; BIOMASS; BIOREACTOR; BIOTECHNOLOGY; CARBOHYDRATE; COLIFORM BACTERIUM; GENE EXPRESSION; GROWTH RATE; INDUSTRIAL PRODUCTION; MICROBIAL ACTIVITY; PROTEIN; SUBSTRATE;

ARTICLE; BACTERIAL GROWTH; BIOREACTOR; BROTH DILUTION; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; LACTOSE OPERON; MICROBIAL BIOMASS; MICROTITER PLATE ASSAY; NONHUMAN; PROTEIN INDUCTION; PROTEIN SYNTHESIS; SOLUBILITY;

BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; PODOVIRIDAE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION;

ESCHERICHIA COLI; LURIA;

EID: 80052628175     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3407-z     Document Type: Article

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