Autoinduction of protein expression

Current Protocols in Protein Science

Volumn , Issue SUPPL. 56, 2009, Pages

  Fox, Brian G ^a   Blommel, Paul G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Escherichia coli; Expression; High throughput methods; Recombinant protein; X ray crystallography

Indexed keywords

LACTOSE; RECOMBINANT PROTEIN; SELENOMETHIONINE; AMINO ACID; ANTIBIOTIC AGENT; CYANOCOBALAMIN; GLUCOSE; GLYCEROL; MAGNESIUM SULFATE; METHIONINE; NITROGEN; PHOSPHORUS; SUCCINIC ACID; SUGAR; SULFUR; TRACE METAL; VITAMIN;

AGAR MEDIUM; BACTERIUM CULTURE; CULTURE MEDIUM; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; REVIEW; ARTICLE; AUTOINDUCTION; CELL SUSPENSION; CHEMICAL LABELING; CHEMICAL MODIFICATION; CHEMICAL PROCEDURES; CONCENTRATION (PARAMETERS); HIGH THROUGHPUT SEQUENCING; PROCESS DEVELOPMENT; PROTEIN EXPRESSION ASSAY; PROTEIN FUNCTION; STRUCTURE ANALYSIS; X RAY ANALYSIS;

BACTERIAL PROTEINS; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; CULTURE MEDIA; ESCHERICHIA COLI; LACTOSE; RECOMBINANT PROTEINS; SELENOMETHIONINE;

ESCHERICHIA COLI;

EID: 65249138782     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps0523s56     Document Type: Review

References (33)

1. Bailey, L.J., Elsen, N.L., Pierce, B.S., and Fox, B.G. 2007. Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase. Protein Expr. Purif. 57:9-16.
2. Blommel, P.G. and Fox, B.G. 2005. Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins. Anal. Biochem. 336:75-86.
3. Blommel, P.G. and Fox, B.G. 2007. A combined approach to improving large-scale production of tobacco etch virus protease. Protein Expr. Purif. 55:53-68.
4. Blommel, P.G., Becker, K.J., Duvnjak, P., and Fox, B.G. 2007. Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition. Biotechnol. Prog. 23:585-598.
5. Broadwater, J.A. and Fox, B.G. 1998. Spinach acyl carrier protein: Phosphopantetheinylation in Escherichia coli BL21(DE3), in vitro acylation, and enzymatic desaturation of histidine-tagged isoform I. Protein Expr. Purif. 15:314-326.
6. Dubendorff, J.W. and Studier, F.W. 1991. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J. Mol. Biol. 219:45-59.
7. Fang, L., Jia, K.Z., Tang, Y.L., Ma, D.Y., Yu, M., and Hua, Z.C. 2007. An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization. Protein Expr. Purif. 51:102-109.
8. Frederick, R.O., Bergeman, L., Blommel, P.G., Bailey, L.J., McCoy, J.G., Song, J., Meske, L., Bingman, C.A., Riters, M., Dillon, N.A., Kunert, J., Yoon, J.W., Lim, A., Cassidy, M., Bunge, J., Aceti, D.J., Primm, J.G., Markley, J.L., Phillips Jr, G.N., and Fox, B.G. 2007. Small-scale, semiautomated purification of eukaryotic proteins for structure determination. J. Struct. Funct. Genomics 8:153-156.
9. Galvao-Botton, L.M., Katsuyama, A.M., Guzzo, C.R., Almeida, F.C., Farah, C.S., and Valente, A.P. 2003. High-throughput screening of structural proteomics targets using NMR. FEBS Lett. 552:207-213.
10. Grossman, T.W., Kawasaki, E.S., Punreddy, S.R., and Osborn, M.S. 1998. Spontaneous cAMPdependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene 209:95-103.
11. Haas, J.A., Frederick, M.A., and Fox, B.G. 2000. Chemical and posttranslational modification of Escherichia coli acyl carrier protein for preparation of dansyl-acyl carrier proteins. Protein Expr. Purif. 20:274-284.
12. Hoffman, B.J., Broadwater, J.A., Johnson, P., Harper, J., Fox, B.G., and Kenealy, W.R. 1995. Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21(DE3): Process control yielding high levels of metal incorporated, soluble protein. Protein Expr. Purif. 6:646-654.
13. Kennedy, M.A., Montelione, G.T., Arrowsmith, C.H., and Markley, J.L. 2002. Role for NMR in structural genomics. J. Struct. Funct. Genomics 2:155-169.
14. Kumar, R.A., and Clark, D.S. 2006. Highthroughput screening of biocatalytic activity: Applications in drug discovery. Curr. Opin. Chem. Biol. 10:162-168.
15. Levin, E.J., Elsen, N.L., Seder, K.D., McCoy, J.G., Fox, B.G., and Phillips, G.N., Jr. 2008. X-ray crystal structure of a soluble Rieske-type ferredoxin from Mus musculus. Acta. Crystallogr. D Biol. Crystallogr. 64:933-940.
16. Lion, N., Reymond, F., Girault, H.H., and Rossier, J.S. 2004. Why the move to microfluidics for protein analysis? Curr. Opin. Biotechnol. 15:31-37.
17. Monod, J. 1972. Nobel Lectures, Physiology or Medicine 1963-1970. Elsevier Publishing Company, Amsterdam.
18. Myers, R.H. and Montgomery, D.C. 2002. Response Surface Methodology: Process and Product Optimization Using Designed Experiments. John Wiley & Sons, New York.
19. Neubauer, P., Wolff, C., Hecker, M., Hoffmann, K., Meyer, L., Kruschke, P., and Heinrich, H.W. 1991. Introduction of the tac-promoter by lactose under fermentation conditions. Acta. Biotechnol. 11:23-29.
20. Neubauer, P., Hofmann, K., Holst, O., Mattiasson, B., and Kruschke, P. 1992. Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as an inducer. Appl. Microbiol. Biotechnol. 36:739-744.
21. Segelke, B. 2005. Macromolecular crystallization with microfluidic free-interface diffusion. Expert Rev. Proteomics 2:165-172.
22. Sharp, P.M. and Li, W.-H. 1987. The codon adaptation index - a measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res. 15:1281-1295.
23. Sobrado, P., Goren, M.A., James, D., and Amundson, C.K. 2007. A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. Protein Expr. Purif. 58:229-241.
24. Sreenath, H.K., Bingman, C.A., Buchan, B.W., Seder, K.D., Burns, B.T., Geetha, H.V., Jeon, W.B., Vojtik, F.C., Aceti, D.J., Frederick, R.O., Phillips, G.N., Jr., and Fox, B.G. 2005. Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium. Protein Expr. Purif. 40:256-267.
25. Studier, F.W. 2005. Protein production by autoinduction in high-density shaking cultures. Protein Expr. Purif. 41:207-234.
26. Studts, J.M. and Fox, B.G. 1999. Application of fed-batch fermentation to the preparation of isotopically labeled- or selenomethioninelabeled proteins. Protein Expr. Purif. 16:109-119.
27. Studts, J.M., Mitchell, K.H., Pikus, J.D., McClay, K., Steffan, R.J., and Fox, B.G. 2000. Optimized expression and purification of toluene 4-monooxygenase hydroxylase. Protein Expr. Purif. 20:58-65.
28. Terpe, K. 2006. Overview of bacterial expression systems for heterologous protein production: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 72:211-222.
29. Thao, S., Zhao, Q., Kimball, T., Steffan, E., Newman, C.S., Fox, B.G., and Wrobel, R.L. 2004. Results from high-throughput DNA cloning of Arabidopsis thaliana target genes by site-specific recombination. J. Struct. Funct. Genomics 5:267-276.
30. 15 N]-labeled proteins for NMR screening and structure determination. Protein Expr. Purif. 40:268-278.
31. van den Berg, S., Lofdahl, P.A., Hard, T., and Berglund, H. 2006. Improved solubility of TEV protease by directed evolution. J. Biotechnol. 121:291-298.
32. van der Woerd, M., Ferree, D., and Pusey, M. 2003. The promise of macromolecular crystallization in microfluidic chips. J. Struct. Biol. 142:180-187.
33. Zheng, B., Gerdts, C.J., and Ismagilov, R.F. 2005. Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization. Curr. Opin. Struct. Biol. 15:548-555.