Inclusion body anatomy and functioning of chaperone-mediated in vivo inclusion body disassembly during high-level recombinant protein production in Escherichia coli

Journal of Biotechnology

Volumn 127, Issue 2, 2007, Pages 244-257

  Rinas, Ursula ^a   Hoffmann, Frank ^b   Betiku, Eriola ^a   Estapé, David ^a   Marten, Sabine ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Chaperones; Disaggregation; Inclusion bodies; Recombinant protein production

Indexed keywords

AGGLOMERATION; CELLS; CONFORMATIONS; ENZYMES; GENETIC ENGINEERING; PHYSIOLOGY; PROTEINS;

CHAPERONES; CYTOPLASMIC INCLUSION BODIES; DENATURANTS; DISAGGREGATION; FIBROBLAST GROWTH FACTOR; RECOMBINANT PROTEIN PRODUCTION;

ESCHERICHIA COLI;

BASIC FIBROBLAST GROWTH FACTOR; CHAPERONE; CHAPERONIN; DEXTRO TAGATOSE 1,6 BISPHOSPHATE ALDOLASE; DIHYDROLIPOAMINE DEHYDROGENASE; ELONGATION FACTOR TU; ISOENZYME; PROTEIN CLPB; PROTEIN DNAK; RECOMBINANT BASIC FIBROBLAST GROWTH FACTOR; RECOMBINANT BASIC FIBROBLAST GROWTH FACTOR 2; TRYPTOPHANASE; UNCLASSIFIED DRUG;

ARTICLE; CELL INCLUSION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; INCUBATION TIME; MOLECULAR INTERACTION; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN CONTENT; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; THERMODYNAMICS;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FIBROBLAST GROWTH FACTOR 2; GROEL PROTEIN; GROES PROTEIN; HSP40 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; INCLUSION BODIES; MOLECULAR CHAPERONES; MUTATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEOME; RECOMBINANT PROTEINS; SOLUBILITY; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 33846222898     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.07.004     Document Type: Article

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