Perspectives on electron cryo-tomography of vitreous cryo-sections

Journal of Electron Microscopy

Volumn 60, Issue SUPPL. 1, 2011, Pages

  Pierson, Jason ^a,b   Vos, Matthijn ^a,b   McIntosh, J Richard ^c   Peters, Peter J ^a,d  

^a ANTONI VAN LEEUWENHOEK HOSPITAL   (Netherlands)

^b Thermo Fisher Scientific   (United States)

^c UNIVERSITY OF COLORADO   (United States)

^d DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

electron cryo tomography; vitreous cryo sectioning

Indexed keywords

ANIMAL; CRYOELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; FREEZING; IMAGE PROCESSING; METHODOLOGY; REVIEW; ULTRAMICROTOMY;

ANIMALS; CRYOELECTRON MICROSCOPY; CRYOULTRAMICROTOMY; ELECTRON MICROSCOPE TOMOGRAPHY; FREEZING; IMAGE PROCESSING, COMPUTER-ASSISTED;

HISTOLOGY; TISSUE; TOMOGRAPHY;

BIOLOGICAL SAMPLES; BIOLOGICAL TISSUES; CELLULAR ORGANIZATION; ELECTRON CRYO-TOMOGRAPHY; MACROMOLECULAR COMPLEXES; SAMPLE PREPARATION; SPATIAL ARRANGEMENTS; STRUCTURAL BIOLOGY; ULTRA-MICROTOMY; VITREOUS CRYO-SECTIONING;

CRYOGENICS;

EID: 80052066186     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfr014     Document Type: Review

References (35)

1. Dubochet J, McDowall A W, Menge B, Schmid E N, and Lickfeld K G (1983) Electron microscopy of frozen-hydrated bacteria. J. Bacteriol. 155: 381-390. (Pubitemid 13070635)
2. Al-Amoudi A, Díez D C, Betts M J, and Frangakis A S (2007) The molecular architecture of cadherins in native epidermal desmosomes. Nature 450: 832-837. (Pubitemid 350231314)
3. Sartori N, Bednar J, and Dubochet J (1996) Electron-beam-induced amorphization of ice III or IX obtained by high-pressure freezing. J. Microsc. 182: 163-168. (Pubitemid 26290753)
4. Sartori Blanc N, Studer D, Ruhl K, and Dubochet J (1998) Electron beam-induced changes in vitreous sections of biological samples. J. Microsc. 192: 194-201. (Pubitemid 28539623)
5. Beck M, Malmstrom J A, Lange V, Schmidt A, Deutsch E W, and Aebersold R (2009) Visual proteomics of the human pathogen Leptospira interrogans. Nat. Meth. 6: 817-823.
6. Brandt F, Etchells S A, Ortiz J O, Elcock A H, Hartl F U, and Baumeister W (2009) The native 3D organization of bacterial polysomes. Cell 136: 261-271.
7. Carlson L A, de Marco A, Oberwinkler H, Habermann A, Briggs J A, Krausslich H G, and Grunewald K (2010) Cryo electron tomography of native HIV-1 budding sites. PLoS Pathog. 6: e1001173.
8. Fischer N, Konevega A L, Wintermeyer W, Rodnina M V, and Stark H (2010) Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy. Nature 466: 329-333.
9. Kuhner S, van Noort V, Betts M J, et al. (2009) Proteome organization in a genome-reduced bacterium. Science 326: 1235-1240.
10. Salje J, Zuber B, and Löwe J (2009) Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation. Science 23: 509-512.
11. Studer D, Graber W, Al-Amoudi A, and Eggli P (2001) A new approach for cryofixation by high-pressure freezing. J. Microsc. 203: 285-294. (Pubitemid 33755967)
12. Kellenberger E (1997) The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation. In: Steinbrecht R A and Zierold K (eds), Cryotechniques in Biological Electron Microscopy, pp 35-63 (Springer, Berlin).
13. Sartori N, Richeter K, and Dubochet J (1993) Vitrification depth can be increased more than 10-fold by high pressure freezing. J. Microsc. 172: 55-61. (Pubitemid 23324040)
14. Gilkey J C and Staehelin L A (1986) Advances in ultrarapid freezing for the preservation of cellular ultrastrucutre. J. Electron Microsc. Tech. 3: 177-210. (Pubitemid 16135202)
15. Dubochet J, Adrian M, Chang J J, Homo J C, Lepault J, McDowall A W, and Schultz P (1988) Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21: 129-228.
16. Al-Amoudi A, Chang J J, Leforestier A, McDowall A W, Salamin L M, Norlen L P O, Richter K, Sartori Blanc N, Studer D, and Dubochet J (2004) Cryo-electron microscopy of vitreous sections. EMBO J. 23: 3583-3588. (Pubitemid 39336280)
17. Marko M, Hsieh C H, Schalek R, Frank J, and Mannella C (2007) Focused-ion-beam thinning of frozen-hydrated biological specimens for cryoelectron microscopy. Nat. Meth. 4: 215-217. (Pubitemid 46358869)
18. Ladinksy M S, Pierson J M, and McIntosh J R (2006) Vitreous cryosectioning of cells facilitated by a micromanipulator. J. Microsc. 224: 129-134. (Pubitemid 44973557)
19. Pierson J, Fernandez J J, Bos E, Amini S, Gnaegi H, Vos M, Bel B, Adolfsen F, Carrascosa J L, and Peters P J (2010) Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box. J. Struct. Biol. 169: 219-225.
20. Pierson J, Ziese U, Sani M, and Peters P J (2010) Exploring vitreous cryo-section-induced compression at the macromolecular level using electron cryo-tomography; 80S yeast ribosomes appear unaffected. J. Struct. Biol. 173: 345-349.
21. Al-Amoudi A, Studer D, and Dubochet J (2005) Cutting artifacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150: 109-121. (Pubitemid 40395307)
22. Zhang P, Bos E, Heymann J, Gnaegi H, Kessel M, Peters P J, and Subramaniam S (2004) Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E.coli engineered to overproduce the chemotaxis receptor Tsr. J Microsc. 216: 76-83. (Pubitemid 39312482)
23. Bouchet-Marquis C, Zuber B, Glynn A M, Eltsov M, Grabenbauer M, Goldie K N, Thomas D, Frangakis A S, Dubochet J, and Chretien D (2007) Visualization of cell microtubules in their native state. Biol. Cell 99: 45-53. (Pubitemid 46099777)
24. Mastronarde D N (2005) Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152: 36-51. (Pubitemid 41384176)
25. Briegel A, Chen S, Koster A J, Plitzko J M, Schwartz C L, and Jensen G J (2010) Correlated light and electron cryo-microscopy. Methods Enzymol. 481: 317-341.
26. Schwartz C L, Sarbash V L, Ataullakhanov F I, McIntosh J R, and Nicastro D (2007) Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J. Microsc. 227: 98-109. (Pubitemid 47372306)
27. van Driel L F, Knoops K, Koster A J, and Valentijn J A (2007) Fluorescent labeling of resin-embedded sections for correlative electron microscopy using tomography-based contrast enhancement. J. Struct. Biol. 161: 459-468.
28. Koster A J, Grimm R, Typke D, Hegerl R, Stoschek A, Walz J, and Baumeister W (1997) Perspectives of molecular and cellular electron tomography. J. Struct. Biol. 120: 276-308. (Pubitemid 28137303)
29. Leis A, Rocket B, Andrees L, and Baumesiter W (2009) Visualizing cells at the nanoscale. Trends Biochem. Sci. 34: 60-70.
30. Pennycook S J and Nellist P D (1999) Z-contrast scanning transmission electron microscopy. In: Rickerby D G, Valdre G, and Valdre U (eds), Impact of Electron and Scanning Probe Microscopy on Materials Research, pp 161-207 (Kluwer Academic Publishers, The Netherlands).
31. Loane R F, Xu P, and Silcox J (1992) Incoherent imaging of zone axis crystals with ADF STEM. Ultramicroscopy 40: 121-138.
32. Williams D B and Carter C B (2009) Transmission Electron Microscopy: A Textbook for Materials Science (Springer, New York).
33. Ziese U, Kubel C, Verkleij A, and Koster A J (2002) Three-dimensional localization of ultrasmall immune-gold labels by HAADF-STEM tomography. J. Struct. Biol. 138: 58-62. (Pubitemid 35300748)
34. Jin L, Milazzo A C, Kleinfelder S, Li S, Leblanc P, Duttweiler F, Bouwer J C, Peltier S T, Ellisman M H, and Xuong N H (2008) Applications of direct detection device in transmission electron microscopy. J. Struct. Biol. 161: 352-358. (Pubitemid 351375099)
35. Spahn C M, Beckmann R, Eswar N, Penczek P A, Sali A, Blobel G, and Frank J (2001) Structure of the 80S ribosome from Saccharomyces cerevisiae-tRNA- ribosome and subunit-subunit interactions. Cell 107: 373-386. (Pubitemid 33049980)