Electron beam-induced changes in vitreous sections of biological samples

Journal of Microscopy

Volumn 192, Issue 2, 1998, Pages 194-201

  Sartori Blanc, N ^a   Studer, D ^a   Ruhl, K ^a   Dubochet, J ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

Beam damage; Cartilage; Cryoelectron microscopy; Cryoultramicrotomy; Erythrocytes; High pressure freezing; Maize; Red blood cells; Vitreous water; Vitrification

Indexed keywords

BLOOD; CARTILAGE; ELECTRON IRRADIATION; GRAIN (AGRICULTURAL PRODUCT); IMAGE ENHANCEMENT; VISIBILITY;

BEAM DAMAGE; BIOLOGICAL SAMPLES; CRYO-ELECTRON MICROSCOPY; CRYOULTRAMICROTOMY; ELECTRON-BEAM; ERYTHROCYTE; HIGH-PRESSURE FREEZING; MAIZE; RED BLOOD CELL; VITREOUS WATER;

SHRINKAGE;

ARTICLE; CELL ORGANELLE; CRYOELECTRON MICROSCOPY; ELECTRON BEAM; ELECTRON MICROSCOPY; ERYTHROCYTE; FREEZING; HYPERBARISM; NONHUMAN; PRIORITY JOURNAL;

EID: 0031767521     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1998.00420.x     Document Type: Article

References (26)

1. Bernhard, W. (1965) Ultramicrotomie à basse température. Ann. Biol. 4, 5-18.
2. Boyum, A. (1964) Separation of white blood cells. Nature, 204, 793-794.
3. Chang, J.J., McDowall, A.W., Lepault, J., Freeman, R., Walter, C.A. & Duhochet, J. (1983) Freezing, sectioning and observation artefacts of frozen hydrated sections for electron microscopy. J. Microsc. 132, 109-123.
4. Ding, B., Turgeon, R. & Parthasarathy, M.V. (1991) Effect of high-pressure freezing on plant microfilament bundles. J. Microsc. 165, 367-376.
5. Dubochet, J., Adrian, M., Chang, J.-J., Homo, J.-C., Lepault, J., McDowall, A.W. & Schultz, P. (1988) Cryo-electron microscopy of vitrified specimens. Quart. Rev. Biophys. 21, 129-228.
6. Dubochet, J., Adrian, M., Chang, J.J., Lepault, J. & McDowall, A.W. (1987) Cryoelectron microscopy of vitrified specimens. Cryotechniques in Biological Electron Microscopy (ed. by R. A. Steinbrecht and K. Zierold), pp. 114-131. Springer, Berlin.
7. Dubochet, J., Lepault, J., Freeman, R., Berriman, J.A. & Homo, J.-C. (1982) Electron microscopy of frozen water and aqueous solutions. J. Microsc. 128, 219-237.
8. Dubochet, J., McDowall, A.W., Menge, B., Schmid, E.N. & Lickfeld, K.G. (1983) Electron microscopy of frozen-hydrated bacteria. J. Bacteriol. 155, 381-390.
9. Eusemann, R., Rose, H. & Dubochet, J. (1982) Electron scattering in ice and organic materials. J. Microsc. 128, 239-249.
10. Frederik, P.M. & Busing, W.M. (1981) Ice crystal damage in frozen thin sections: freezing effects and their restoration. J. Microsc. 121, 191-199.
11. Leforestier, A., Richter, K., Livolant, F. & Dubochet, J. (1996) Comparison of slam-freezing and high pressure freezing effects on the DNA cholesteric liquid crystalline structure. J. Microsc. 184, 4-13.
12. McDowall, A.W., Chang, J.-J., Freeman, R., Lepault, J., Walter, C.A. & Dubochet, J. (1983) Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples. J. Microsc. 131, 1-9.
13. McDowall, A.W., Hofmann, W., Lepault, L., Adrian, M. & Dubochet, J. (1984) Cryo-electron microscopy of vitrified insect flight muscle. J. Mol. Biol. 178, 105-111.
14. Michel, M., Gnägi, H. & Müller, M. (1992) Diamonds are a cryosectioner's best friend. J. Microsc. 166, 43-56.
15. Michel, M., Hillmann, T. & Müller, M. (1991) Cryosectioning of plant material frozen at high pressure. J. Microsc. 163, 5-18.
16. Miller, A. (1976) Molecular packing in collagen fibrils. Biochemistry of Collagen (ed. by G. N. Ramachandran and A. H. Reddi), pp. 85-136. Plenum, New York.
17. Moor, H. (1987) Theory and practice of high pressure freezing. Cryotechniques in Biological Electron Microscopy led. by R. A. Steinbrecht and K. Zierold). pp. 175-191. Springer-Verlag. Berlin.
18. Richter, K. (1994a) A cryoglue to mount vitreous biological specimens for cryoultramicrotomy at 110K. J. Microsc. 173, 143-147.
19. Richter, K. (1994b) Cutting artefacts on ultrathin cryosections of biological bulk specimens. Micron. 25, 297-308.
20. Richter, K. & Dubochet, J. (1991) Surface morphology of vitrified ultrathin sections. Experientia, 47, A55.
21. Sartori, N., Richter, K. & Dubochet, J. (1993) Vitrification depth can be increased more than 10-fold by high-pressure freezing. J. Microsc. 172, 55-61.
22. Sartori, N. & Salamin Michel, L.A. (1994) Cryo-transmission electron microscopy of thin vitrified sections. Cell Biology: a Laboratory Handbook (ed. by E. Celis), pp. 177-185. Academic Press, Inc, New York.
23. Studer, D., Michel, M. & Müller, M. (1989) High pressure freezing comes of age. Scanning Microsc. 3, 253-269.
24. Studer, D., Michel, M., Wohlvend, M., Hunziker, E.B. & Buschmann, M. (1995) Vitrification of articular cartilage by high-pressure freezing. J. Microsc. 179, 321-332.
25. Talmon, Y., Adrian, M. & Dubochet, J. (1986) Electron beam radiation damage to organic inclusions in vitreous, cubic, and hexagonal ice. J. Microsc. 141, 375-384.
26. Zierold, K. (1984) The morphology of ultrathin cryosections. Ultramicroscopy, 14, 201-210.