Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation

Science

Volumn 323, Issue 5913, 2009, Pages 509-512

  Salje, Jeanne ^a,b   Zuber, Benoît ^a   Löwe, Jan ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA;

BACTERIUM; CHROMOSOME; DNA; ELECTRON MICROSCOPY; PLASMID;

ARTICLE; BACTERIAL CELL; CHROMOSOME; CRYOELECTRON MICROSCOPY; DIFFRACTION; ESCHERICHIA COLI; GENE OVEREXPRESSION; IN VIVO CULTURE; NONHUMAN; PAIRED HELICAL FILAMENT; PARTITION COEFFICIENT; PRIORITY JOURNAL;

ACTINS; CRYOELECTRON MICROSCOPY; CYTOSKELETON; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; PLASMIDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 58849121358     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1164346     Document Type: Article

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22. We thank K. Gerdes (Newcastle, UK) and J. Møller-Jensen (Odense, Denmark) for kindly supplying plasmids and strains and W. Kühlbrandt and D. Mills (Frankfurt, Germany) for generously allowing us the use of their microscope. J.S. is supported by MRC and Leverhulme Trust studentships, and B.Z. is supported by a European Molecular Biology Organization long-term fellowship. J.S. performed all experiments and their analysis except the initial cryo-sectioning that was done with B.Z.