Three-dimensional localization of ultrasmall immuno-gold labels by HAADF-STEM tomography

Journal of Structural Biology

Volumn 138, Issue 1-2, 2002, Pages 58-62

  Ziese, Ulrike ^a   Kübel, Christian ^b   Verkleij, Arie J ^a   Koster, Abraham J ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b Thermo Fisher Scientific   (United States)

Author keywords

3D reconstruction; Electron tomography; High angular annular dark field scanning TEM; Tilt series; Ultrasmall immuno gold labels

Indexed keywords

GOLD; LEAD; OSMIUM; SILVER; URANIUM;

ACCURACY; ANIMAL CELL; ANTIGEN DETECTION; CALCULATION; CELL ULTRASTRUCTURE; CONFERENCE PAPER; CONTROLLED STUDY; ELECTRON; IMMUNOGOLD LABELING; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; TOMOGRAPHY; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; ARTICLE; CELL STRUCTURE; COMPUTER ASSISTED TOMOGRAPHY; EUKARYOTIC FLAGELLUM; FROG; IMMUNOHISTOCHEMISTRY; METHODOLOGY; NANOTECHNOLOGY; SCANNING TRANSMISSION ELECTRON MICROSCOPY; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

BOVICHTUS; CAPRA HIRCUS; ORYCTOLAGUS CUNICULUS;

ANIMALS; CELLULAR STRUCTURES; CILIA; IMAGING, THREE-DIMENSIONAL; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON, SCANNING TRANSMISSION; NANOTECHNOLOGY; RANA CATESBEIANA; TOMOGRAPHY, X-RAY COMPUTED;

EID: 0036421206     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(02)00018-7     Document Type: Conference Paper

References (21)

1. Baba, N., Terayama, K., Yoshimizu, T., Ichise, N., Tanaka, N., 2001. An auto-tuning method for focusing and astigmatism correction in HAADF-STEM, based on the image contrast transfer function. J. Electron Microsc. 50, 163-176.
2. Beorchia, A., Heliot, L., Menager, M., Kaplan, H., Ploton, D., 1993. Applications of medium voltage STEM for the 3-D study of organelles within very thick sections. J. Microsc. 170, 247-258.
3. Danscher, G., 1981. Histochemical demonstration of heavy metals. A revised version of the silver method suitable for both light and electron microscopy. Histochemistry 71, 1-16.
4. Fung, J.C., Liu, W., de Ruijter, W.J., Chen, H., Abbey, C.K., Sedat, J.W., Agard, D.A., 1996. Toward fully automated high-resolution electron tomography. J. Struct. Biol. 116, 181-189.
5. Hainfeld, J.F., 1990. STEM analysis of Janssen aurooprobe one. In: Bailey, G. (Ed.), Proceedings of the XIIth International Congress on Electron Microscopy. San Francisco Press, San Francisco, pp. 954-955.
6. Hainfeld, J.F., Powell, R.D., 2000. New frontiers in gold labeling. J. Histochem. Cytochem. 48, 471-480.
7. Hainfeld, J.F., Powell, R.D., Stein, J.L., Hacker, G.W., Hauser-Kronberger, C., Cheung, A., Schöfer, C., 1999. Gold-based autometallography. In: Bailey, G.W., Jerome, W.G., McKernan, S., Mansfield, J.F., Price, R.L. (Eds.), Proc. 57th Annual Meeting of the Microscopy Society of America. Springer-Verlag, Berlin/New York, pp. 486-487.
8. Hudspeth, A.J., 1997. How hearing happens. Neuron 19, 947-950.
9. Humbel, B.M., de Jong, M.D., Müller, W.H., Verkleij, A.J., 1998. Pre-embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc. Res. Tech. 42, 43-58.
10. Koster, A.J., Chen, H., Sedat, J.W., Agard, D.A., 1992. Automated microscopy for electron tomography. Ultramicroscopy 46, 207-227.
11. Koster, A.J., de Ruiter, W.J., van den Bos, A., van der Mast, K.D., 1989. Autotuning of a TEM using minimum dose. Ultramicroscopy 27, 251-272.
12. Koster, A.J., van den Bos, A., van der Mast, K.D., 1987. An autotuning method for a TEM. Ultramicroscopy 21, 209-222.
13. Kremer, J.R., Mastronarde, D.N., McIntosh, J.R., 1996. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71-76.
14. Midgley, P.A., Weyland, M., Thomas, J.M., Johnson, B.F.G., 2001. Z-contrast tomography: A technique in three-dimensional nanostructural analysis based on Rutherford scattering. Chem. Commun., 907-908.
15. Radermacher, M., 1992. Weighted back-projection methods. In: Frank, J. (Ed.), Electron Tomography. Plenum Press, New York, pp. 91-115.
16. Reimer, L., 1997. Electron-probe broadening by multiple scattering. In: Transmission Electron Microscopy, Fourth Ed. Springer-Verlag, Berlin, pp. 19-194.
17. Starink, J., Humbel, B., Verkleij, A.J., 1995. Three-dimensional localization of immunogold markers using two tilted electron microscope recordings. Biophys. J. 68, 2171-2180.
18. Stierhoff, Y.D., Humbel, B.M., Homann, R., Otten, M.T., Schwarz, H., 1992. Direct visualization and silver enhancement of ultra-small gold tagged antibodies on immunolabelled ultrathin resin sections. Scanning Microsc. 6, 1009-1022.
19. Tokuyasu, K.T., 1973. A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57, 551-565.
20. Verkleij, A.J., Koster, A.J., Müller, W.H., Humbel, B.M., 1999. Immuno-gold labeling in transmission electron microscopy. In: Thiery, J.P. (Ed.), Molecular Mechanisms of Transcellular Signaling. IOS Press, pp. 339-356.
21. Ziese, U., Janssen, A.H., Murk, J.L., Geerts, W.J.C., Krift, T., Verkleij, A.J., Koster, A.J., 2002. Automated high-throughput electron tomography by pre-calibration of image shifts. J. Microscopy 205 (2), 187-200.