X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: Catalytic mechanism and a new protein superfamily

EMBO Journal

Volumn 19, Issue 20, 2000, Pages 5269-5280

  Ünligil, Uluǧ M ^a   Zhou, Sihong ^a   Yuwaraj, Sivashankary ^a   Sarkar, Mohan ^b   Schachter, Harry ^a,b   Rini, James M ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Evolution; Glycosylation; Glycosyltransferases; N acetylglucosaminyltransferase I; X ray crystal structure

Indexed keywords

GLYCOSIDASE; GLYCOSYLTRANSFERASE; MANGANESE; N ACETYLGLUCOSAMINYLTRANSFERASE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

ANIMAL CELL; ARTICLE; BACILLUS SUBTILIS; CATTLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ESCHERICHIA COLI; MOLECULAR EVOLUTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FOLDING; RABBIT; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

ANIMALIA; BACILLUS SUBTILIS; BOS TAURUS; BOVINAE; ESCHERICHIA COLI; MAMMALIA; METAZOA; ORYCTOLAGUS CUNICULUS;

EID: 0034675845     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.20.5269     Document Type: Article

References (73)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. β-Glucosyltransferase and phosphorylase reveal their common theme
3. A fast algorithm for rendering space-filling molecule pictures
4. ALSCRIPT a tool to format multiple sequence alignments
5. Biological coordination chemistry of magnesium, sodium, and potassium ions. Protein and nucleotide binding sites
6. Structure-function studies of glycosyltransferases
7. Sequence-function relationships of prokaryotic and eukaryotic galactosyltransferases
8. Crystal structure of the bifunctional N-acetylglucosamine 1-phosphate uridyltransferase from Escherichia coli: A paradigm for the related pyrophosphorylase superfamily
9. Crystallography and NMR system: A new software suite for macromolecular structure determination
10. A common motif of eukaryotic glycosyltransferases is essential for the enzyme activity of large clostridial cytotoxins
11. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities
12. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities
13. Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms
14. Carbohydrate-deficient glycoprotein syndrome type II. An autosomal recessive N-acetylglucosaminyltransferase II deficiency different from typical hereditary erythroblastic multinuclearity, with a positive acidified-serum lysis test (HEMPAS)
15. Magnesium activation of nuclease enzymes - The importance of water
16. 'dm': An automated procedure for phase improvement by density modification
17. Glycosyl transfer
18. Snapshots along an enzymatic reaction coordinate: Analysis of a retaining β-glycoside hydrolase
19. Evolving views of protein glycosylation
20. Competitive binding in magnesium coordination chemistry: Water versus ligands of biological interest
21. Crystal structures of the bovine β4-galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose
22. Suppression of tumor growth and metastasis in Mgat5-deficient mice
23. The 1.9 Å crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis
24. Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:α-D-mannoside β2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:α-D-mannoside β2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity
25. Identification that KfiA, a protein essential for the biosynthesis of the Escherichia coli K5 capsular polysaccharide, is an α-UDP-GlcNAc glycosyltransferase: The formation of a membrane associated K5 biosynthetic complex requires KfiA, KfiB and KfiC
26. Protein structure comparison by alignment of distance matrices
27. Evolutionary link between glycogen phosphorylase and a DNA modifying enzyme
28. Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates
29. The carbohydrate-deficient glycoprotein syndromes: Pre-Golgi and Golgi disorders?
30. Carbohydrate deficient glycoprotein syndrome type II: A deficiency in Golgi localised N-acetyl-glucosaminyltransferase II
31. A new approach to protein fold recognition
32. Improved methods for building protein models in electron density maps and the location of errors in these models
33. Analysis of the galactosyltransferase reaction by positional isotope exchange and secondary deuterium isotope effects
34. Phosphate-metal ion interactions of nucleotides and polynucleotides
35. Efficient rebuilding of protein structures
36. Structure of glycoproteins and their oligosaccharide units
37. Maximum-likelihood heavy-atom parameter refinement in the MIR and MAD methods
38. Raster3D version 2.0, a program for photorealistic molecular graphics
39. Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development
40. Control of glycoprotein synthesis: Substrate specificity of rat liver UDP-GlcNAc:Manα3R β2-N-acetylglucosaminyltransferase I using synthetic substrate analogues
41. T4 phage β-glucosyltransferase: Substrate binding and proposed catalytic mechanism
42. Mechanism and specificity of human α-1,3-fucosyltransferase V
43. Mechanism of human α-1,3-fucosyltransferase V: Glycosidic cleavage occurs prior to nucleophilic attack
44. SCOP: A structural classification of proteins database for the investigation of sequences and structures
45. Control of glycoprotein synthesis. Lectin-resistant mutant containing only one of two distinct N-acetylglucosaminyltransferase activities present in wild type Chinese hamster ovary cells
46. Control of glycoprotein synthesis. Purification and characterization of rabbit liver UDP-N-acetylglucosamine:α-3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I
47. Exploring the cellulose/xylan specificity of the β-1,4-glycanase Cex from Cellulomonas fimi through crystallography and mutation
48. Genetic defect in N-acetylglucosaminyltransferase I gene of a ricin-resistant baby hamster kidney mutant
49. Processing of X-ray diffraction data collected in oscillation mode
50. Glycosylation defect in Lec1 Chinese hamster ovary mutant is due to a point mutation in N-acetylglucosaminyltransferase I gene
51. Synergistic inhibition of human α-1,3-fucosyltransferase V
52. Synthetic substrate analogues for UDP-GlcNAc:Manα1-3R β1-2-N-acetylglucosaminyltransferase I. Substrate specificity and inhibitors for the enzyme
53. Chemical and biological evolution of nucleotide-binding protein
54. Multiple protein sequence alignment from tertiary structure comparison: Assignment of global and residue confidence levels
55. Catalysis and specificity in enzymatic glycoside hydrolysis: A 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase
56. Expression of recombinant rabbit UDP-GlcNAc: α3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I catalytic domain in Sf9 insect cells
57. Removal of 106 amino acids from the N-terminus of UDP-GlcNAc: α-3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I does not inactivate the enzyme
58. Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides
59. The 'yellow brick road' to branched complex N-glycans
60. Conserved structural regions involved in the catalytic mechanism of Escherichia coli K-12 WaaO (RfaI)
61. Four critical aspartic acid residues potentially involved in the catalytic mechanism of Escherichia coli K-12 WaaR
62. Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase
63. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-glycoprotein N-acetylglucosaminyltransferase activity
64. The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 Å resolution
65. Mutations in the MGAT2 gene controlling complex N-glycan synthesis cause carbohydrate-deficient glycoprotein syndrome type II, an autosomal recessive disease with defective brain development
66. Automated MAD and MIR structure solution
67. Biological roles of oligosaccharides: All of the theories are correct
68. Control of glycoprotein synthesis. IX. A terminal Man α1-3Manβ1-sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: α-D-mannoside (GlcNAc to Manα1-3) β2-N-acetylglucosaminyltransferase I
69. Crystal structure of the DNA modifying enzyme β-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose
70. Activity of the yeast MNN1 α-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases
71. Glycosidase mechanisms: Anatomy of a finely tuned catalyst