A novel expression and purification system for the production of enzymatic and biologically active human granzyme B

Journal of Immunological Methods

Volumn 371, Issue 1-2, 2011, Pages 8-17

  Gehrmann, Mathias ^a   Doß, Brigitte T ^a,b   Wagner, Michaela ^b   Zettlitz, Kirstin A ^c   Kontermann, Roland E ^a   Foulds, Gemma ^a,d   Pockley, Alan G ^d   Multhoff, Gabriele ^a,b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

^c UNIVERSITY OF STUTTGART   (Germany)

^d UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Activity; Enterokinase; Granzyme B; HEK293 cells; Neuraminic acid; Purification

Indexed keywords

ENTEROPEPTIDASE; GRANZYME B; HEAT SHOCK PROTEIN 70; HEPARIN; NICKEL; PERFORIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CELL STRAIN HEK293; CONTROLLED STUDY; DRUG PURIFICATION; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSFECTION; GLYCOSYLATION; HOST CELL; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PROCESSING; TUMOR CELL;

ANIMALS; APOPTOSIS; CELL LINE, TUMOR; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION; GENETIC VECTORS; GLYCOSYLATION; GRANZYMES; HEK293 CELLS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; MICE; NEURAMINIC ACIDS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

MAMMALIA;

EID: 79960964063     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2011.06.007     Document Type: Article

References (31)

1. Caputo A., Parrish J.C., James M.N., Powers J.C., Bleackley R.C. Electrostatic reversal of serine proteinase substrate specificity. Proteins 1999, 35:415.
2. Chowdhury D., Lieberman J. Death by a thousand cuts: granzyme pathways of programmed cell death. Annu. Rev. Immunol. 2008, 26:389.
3. Cullen S.P., Brunet M., Martin S.J. Granzymes in cancer and immunity. Cell Death Differ. 2010, 17:616.
4. Dalken B., Giesubel U., Knauer S.K., Wels W.S. Targeted induction of apoptosis by chimeric granzyme B fusion proteins carrying antibody and growth factor domains for cell recognition. Cell Death Differ. 2006, 13:576.
5. Dressel R., Raja S.M., Höning S., Seidler T., Froelich C.J., von Figura K., Günther E. Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells. J. Biol. Chem. 2004, 279:20200.
6. Elmore S. Apoptosis: a review of programmed cell death. Toxicol. Pathol. 2007, 35:495.
7. Froelich C.J., Orth K., Turbov J., Seth P., Gottlieb R., Babior B., Shah G.M., Bleackley R.C., Dixit V.M., Hanna W. New paradigm for lymphocyte granule-mediated cytotoxicity: target cells bind and internalize granzyme B, but an endosomolytic agent is necessary for cytosolic delivery and subsequent apoptosis. J. Biol. Chem. 1996, 271:29073.
8. Giesubel U., Dalken B., Mahmud H., Wels W.S. Cell binding, internalization and cytotoxic activity of human granzyme B expressed in the yeast Pichia pastoris. Biochem. J. 2006, 394:563.
9. Gross C., Koelch W., DeMaio A., Arispe N., Multhoff G. Cell surface-bound heat shock protein 70 (Hsp70) mediates perforin-independent apoptosis by specific binding and uptake of granzyme B. J. Biol. Chem. 2003, 278:41173.
10. Hanna W.L., Zhang X., Turbov J., Winkler U., Hudig D., Froelich C.J. Rapid purification of cationic granule proteases: application to human granzymes. Protein Expr. Purif. 1993, 4:398.
11. Hoves S., Trapani J.A., Voskoboinik I. The battlefield of perforin/granzyme cell death pathways. J. Leukoc. Biol. 2010, 87:237.
12. Kurschus F.C., Jenne D.E. Delivery and therapeutic potential of human granzyme B. Immunol. Rev. 2010, 235:159.
13. Kurschus F.C., Bruno R., Fellows E., Falk C.S., Jenne D.E. Membrane receptors are not required to deliver granzyme B during killer cell attack. Blood 2005, 105:2049.
14. Kurschus F.C., Fellows E., Stegmann E., Jenne D.E. Granzyme B delivery via perforin is restricted by size, but not by heparan sulfate-dependent endocytosis. Proc. Natl. Acad. Sci. U.S.A. 2008, 105:13799.
15. Lorentsen R.H., Fynbo C.H., Thogersen H.C., Etzerodt M., Holtet T.L. Expression, refolding, and purification of recombinant human granzyme B. Protein Expr. Purif. 2005, 39:18.
16. Motyka B., Korbutt G., Pinkoski M.J., Heibein J.A., Caputo A., Hobman M., Barry M., Shostak I., Sawchuk T., Holmes C.F., Gauldie J., Bleackley R.C. Mannose 6-phosphate/insulin-like growth factor II receptor is a death receptor for granzyme B during cytotoxic T cell-induced apoptosis. Cell 2000, 103:491.
17. Pardo J., Aguilo J.I., Anel A., Martin P., Joeckel L., Borner C., Wallich R., Mullbacher A., Froelich C.J., Simon M.M. The biology of cytotoxic cell granule exocytosis pathway: granzymes have evolved to induce cell death and inflammation. Microbes Infect. 2009, 11:452.
18. Pipkin M.E., Lieberman J. Delivering the kiss of death: progress on understanding how perforin works. Curr. Opin. Immunol. 2007, 19:301.
19. Shi L., Yang X., Froelich C.J., Greenberg A.H. Purification and use of granzyme B. Methods Enzymol. 2000, 322:125.
20. Smyth M.J., McGuire M.J., Thia K.Y. Expression of recombinant human granzyme B. A processing and activation role for dipeptidyl peptidase I. J. Immunol. 1995, 154:6299.
21. Stangl S., Gehrmann M., Dressel R., Alves F., Dullin C., Themelis G., Ntziachristos V., Staeblein E., Walch A., Winkelmann I., Multhoff G. In vivo imaging of CT26 mouse tumors by using cmHsp70.1 monoclonal antibody. J. Cell. Mol. Med. 2010, 108(2):733.
22. Stangl S., Gehrmann M., Riegger J., Kuhs K., Riederer I., Sievert W., Hube K., Mocikat R., Dressel R., Kremmer E., Pockey A.G., Friedrich L., Vigh L., Skerra A., Multhoff G. Targeting membrane Hsp70 on tumors by cmHsp70.1 antibody. Proc. Natl. Acad. Sci. U.S.A. 2011, 108:733.
23. Sun J., Bird C.H., Buzza M.S., McKee K.E., Whisstock J.C., Bird P.I. Expression and purification of recombinant human granzyme B from Pichia pastoris. Biochem. Biophys. Res. Commun. 1999, 261:251.
24. Thomas D.A., Scorrano L., Putcha G.V., Korsmeyer S.J., Ley T.J. Granzyme B can cause mitochondrial depolarization and cell death in the absence of BID, BAX, and BAK. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:14985.
25. Trapani J.A., Klein J.L., White P.C., Dupont B. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 1988, 85:6924.
26. Trapani J.A., Browne K.A., Dawson M., Smyth M.J. Immunopurification of functional Asp-ase (natural killer cell granzyme B) using a monoclonal antibody. Biochem. Biophys. Res. Commun. 1993, 195:910.
27. Trapani J.A., Smyth M.J., Apostolidis V.A., Dawson M., Browne K.A. Granule serine proteases are normal nuclear constituents of natural killer cells. J. Biol. Chem. 1994, 269:18359.
28. Veugelers K., Motyka B., Goping I.S., Shostak I., Sawchuk T., Bleackley R.C. Granule-mediated killing by granzyme B and perforin requires a mannose 6-phosphate receptor and is augmented by cell surface heparan sulfate. Mol. Biol. Cell 2006, 17:623.
29. Xia Z., Kam C.M., Huang C., Powers J.C., Mandle R.J., Stevens R.L., Lieberman J. Expression and purification of enzymatically active recombinant granzyme B in a baculovirus system. Biochem. Biophys. Res. Commun. 1998, 243:384.
30. Zettlitz K.A., Lorenz V., Landauer K., Munkel S., Herrmann A., Scheurich P., Pfizenmaier K., Kontermann R. ATROSAB, a humanized antagonistic anti-tumor necrosis factor receptor one-specific antibody. MAbs 2010, 2:639.
31. Zhao J., Zhang L.H., Jia L.T., Zhang L., Xu Y.M., Wang Z., Yu C.J., Peng W.D., Wen W.H., Wang C.J., Chen S.Y., Yang A.G. Secreted antibody/granzyme B fusion protein stimulates selective killing of HER2-overexpressing tumor cells. J. Biol. Chem. 2004, 279:21343.