Discrimination of viable acanthamoeba castellani trophozoites and cysts by propidium monoazide real-time polymerase chain reaction

Journal of Eukaryotic Microbiology

Volumn 58, Issue 4, 2011, Pages 359-364

  Fittipaldi, Mariana ^a   Pino Rodriguez, Nancy J ^a,b   Adrados, Bárbara ^a   Agustí, Gemma ^a   Peñuela, Gustavo ^b   Morató, Jordi ^a   Codony, Francesc ^a  

^a UNIVERSITAT POLITÈCNICA DE CATALUNYA   (Spain)

^b UNIVERSIDAD DE ANTIOQUIA   (Colombia)

Author keywords

Amoebae; contact lens solutions; differentiation; PMA; real time PCR; viable pathogen

Indexed keywords

AZIDE; CONTACT LENS SOLUTION; DRUG DERIVATIVE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; PROTOZOAL DNA;

BIOMONITORING; CELL ORGANELLE; DISCRIMINANT ANALYSIS; DISEASE TREATMENT; DISINFECTION; MICROBIOLOGY; PATHOGENICITY; POLYMERASE CHAIN REACTION; PROTOZOAN; QUANTITATIVE ANALYSIS; SURVIVAL;

ACANTHAMOEBA CASTELLANII; ARTICLE; CYTOLOGY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; STAINING; TROPHOZOITE;

ACANTHAMOEBA CASTELLANII; AZIDES; CONTACT LENS SOLUTIONS; DNA, PROTOZOAN; POLYMERASE CHAIN REACTION; PROPIDIUM; STAINING AND LABELING; TROPHOZOITES;

ACANTHAMOEBA CASTELLANII; BACTERIA (MICROORGANISMS);

EID: 79960449862     PISSN: 10665234     EISSN: 15507408     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2011.00557.x     Document Type: Article

References (56)

1. Agusti, G., Codony, F., Fittipaldi, M., Adrados, B., &, Morató, J., 2010. Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR. Helicobacter, 15: 473-476.
2. Amstrong, M., 2000. The pathogenesis of human Acanthamoeba infection. Infect. Dis. Rev., 2: 65-73.
3. Andorrà, I., Esteve-Zarzoso, B., Guillamón, J. M., &, Mas, A., 2010. Determination of viable wine yeast using DNA binding dyes and quantitative PCR. Int. J. Food Microbiol., 144: 257-262.
4. Bae, S., &, Wuertz, S., 2009. Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide. Appl. Environ. Microbiol., 75: 2940-2944.
5. Beattie, T. K., Seal, D. V., Tomlinson, A., McFadyen, A. K., &, Grimason, A. M., 2003. Determination of amoebicidal activities of multipurpose contact lens solutions by using a most probable number enumeration technique. J. Clin. Microbiol., 41: 2992-3000. (Pubitemid 36842290)
6. Behets, J., Seghi, F., Declerck, P., Verelst, L., Duvivier, L., Van Damme, A., &, Ollevier, F., 2003. Detection of Naegleria spp. and Naegleria fowleri: a comparison of flagellation tests, ELISA and PCR. Water Sci. Technol., 47: 117-122. (Pubitemid 36300848)
7. Cawthorn, D. M., &, Witthuhn, R. C., 2008. Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazide. Appl. Environ. Microbiol., 105: 1178-1185.
8. Chang, C. W., Wu, C. Y., &, Ming, K. W., 2010. Evaluation of real-time PCR methods for quantification of Acanthamoeba in anthropogenic water and biofilms. J. Appl. Microbiol., 109: 799-807.
9. Dannelley, J. M., Boyce, L., &, Gaubatz, W., 1986. Efficiency of photo-affinity labeling DNA homopolymers and copolymers with ethidium monoazide. Photochem. Photobiol., 43: 7-11.
10. Declerck, P., Behets, J., Van Hoef, V., &, Ollevier, F., 2007. Detection of Legionella spp. and some of their amoeba hosts in floating biofilms from anthropogenic and natural aquatic environments. Water Res., 41: 3159-3167. (Pubitemid 46935811)
11. Delgado Viscogliosi, P., Solignac, L., &, Delattre, J. M., 2009. Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples. Appl. Environ. Microbiol., 75: 3502-3512.
12. Elhafi, G., Naylor, C. J., Savage, C. E., &, Jones, R. C., 2004. Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction. Avian Pathol., 33: 300-306.
13. Fittipaldi, M., Codony, F., Adrados, B., Camper, A. K., &, Morató, J., 2011. Viable real-time PCR in environmental samples: can all data be interpreted directly? Microb. Ecol., 61: 7-12.
14. Fittipaldi, M., Rodriguez, N. J., Codony, F., Adrados, B., Peñuela, G., &, Morató, J., 2010. Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR. J. Virol. Methods, 168: 228-232.
15. Graiver, D. A., Saunders, S. E., Topliff, C. L., Kelling, C. L., &, Bartelt-Hunt, S. L., 2010. Ethidium monoazide does not inhibit RT-PCR amplification of non-viable avian influenza RNA. J. Virol. Methods, 164: 51-54.
16. Greub, G., &, Raoult, D., 2004. Microorganisms resistant to free-living amoebae. Clin. Microbiol. Rev., 17: 413-433. (Pubitemid 38534035)
17. Hammersmith, K. M., 2006. Diagnosis and management of Acanthamoeba keratitis. Curr. Opin. Ophthalmol., 17: 327-331. (Pubitemid 44215039)
18. Hiti, K., Walochnik, J., Haller-Schober, E. M., Faschinger, C., &, Aspöck, H., 2002. Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. Br. J. Ophthalmol., 86: 144-146. (Pubitemid 34127535)
19. Horn, M., &, Wagner, M., 2004. Bacterial endosymbionts of free-living amoebae. J. Eukaryot. Microbiol., 51: 509-514. (Pubitemid 39348558)
20. Johnston, S. P., Sriram, R., Qvarnstrom, Y., Roy, S., Verani, J., Yoder, J., Lorick, S., Roberts, J., Beach, M. J., &, Visvesvara, G., 2009. Resistance of Acanthamoeba cysts to disinfection in multiple contact lens solutions. J. Clin. Microbiol., 47: 2040-2045.
21. Jung, S. Y., Alsam, S., Kim, K. S., &, Khan, N. A., 2008. Pathogen-pathogen interactions: a comparative study of Escherichia coli interactions with the clinical and environmental isolates of Acanthamoeba. World J. Microbiol. Biotechnol., 24: 2339-2348.
22. Kahane, S., Dvoskin, B., Mathias, M., &, Friedman, M. G., 2001. Infection of Acanthamoeba polyphaga with Simkania negevensis and S. negevensis survival within amoebal cysts. Appl. Environ. Microbiol., 67: 4789-4795. (Pubitemid 33646114)
23. Khan, N. A., 2006. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol. Rev., 30: 564-595. (Pubitemid 43855767)
24. Khan, N. A., Jarroll, E. L., &, Paget, T. A., 2001. Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. Curr. Microbiol., 43: 204-208. (Pubitemid 32642122)
25. Kobayashi, H., Oethinger, M., Tuohy, M. J., Hall, G. S., &, Bauer, T. W., 2010. Distinction between intact and antibiotic-inactivated bacteria by real-time PCR after treatment with propidium monoazide. J. Orthop. Res., 28: 1245-1251.
26. Kralik, P., Nocker, A., &, Pavlik, I., 2010. Mycobacterium avium subsp. Paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment. Int. J. Food Microbiol., 141 (Suppl. 1): S80-S86.
27. Loret, J. F., &, Greub, G., 2010. Free-living amoebae: biological by-passes in water treatment. Int. J. Hyg. Environ. Health, 213: 167-175.
28. Ly, T. M., &, Muller, H. E., 1990. Ingested Listeria monocytogenes survive and multiply in protozoa. J. Med. Microbial., 33: 51-54. (Pubitemid 20289848)
29. Marciano-Cabral, F., &, Cabral, G., 2003. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev., 16: 273-307. (Pubitemid 36519813)
30. Marshall, M. M., Naumovitz, D., Ortega, Y., &, Sterling, C. R., 1997. Waterborne protozoan pathogens. Clin. Microbiol. Rev., 10: 67-85. (Pubitemid 27042832)
31. Nocker, A., &, Camper, A. K., 2009. Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol. Lett., 291: 137-142.
32. Nocker, A., Sossa, K. E., &, Camper, A. K., 2007. Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. J. Microbiol. Methods, 70: 252-260. (Pubitemid 47198131)
33. Nocker, A., Mazza, A., Masson, L., Camper, A. K., &, Brousseau, R., 2009. Selective detection of live bacteria combining propidium monoazide sample treatment with microarray technology. J. Microbiol. Methods, 76: 253-261.
34. Obritsch, M. D., Fish, D. N., MacLaren, R., &, Jung, R., 2005. Nosocomial infections due to multidrug-resistant Pseudomonas aeruginosa: epidemiology and treatment options. Pharmacotherapy, 25: 1353-1364. (Pubitemid 41407633)
35. Pagnier, I., Merchat, M., &, La Scola, B., 2009. Potentially pathogenic amoeba-associated microorganisms in cooling towers and their control. Future Microbiol., 4: 615-629.
36. Pan, Y., &, Breidt, F., 2007. Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl. Environ. Microbiol., 73: 8028-8031. (Pubitemid 350308823)
37. Pasricha, G., Sharma, S., Garg, P., &, Aggarwal, R. K., 2003. Use of 18S rRNA gene-based PCR assay for diagnosis of Acanthamoeba keratitis in non-contact lens wearers in India. J. Clin. Microbiol., 41: 3206-3211. (Pubitemid 36842323)
38. Qvarnstrom, Y., Visvesvara, G. S., Sriram, R., &, da Silva, A. J., 2006. Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. J. Clin. Microbiol., 44: 3589-3595. (Pubitemid 46768828)
39. Radford, C. F., Lehmann, O. J., &, Dart, J. K. G., 1998. Acanthamoeba keratitis: multicentre survey in England 1992-1996. Br. J. Ophthalmol., 82: 1387-1392. (Pubitemid 28556523)
40. Rawsthorne, H., Dock, C. N., &, Jaykus, L. A., 2009. PCR-Based method using propidium monoazide to distinguish viable from non-viable Bacillus subtilis spores. Appl. Environ. Microbiol., 75: 2936-2939.
41. Rieder, A., Schwartz, T., Schön-Hölz, K., Marten, S. M., SüB, J., Gusbeth, C., Kohnen, W., Swoboda, W., Obst, U., &, Frey, W., 2008. Molecular monitoring of inactivation efficiencies of bacteria during pulsed electric field treatment of clinical wastewater. J. Appl. Microbiol., 105: 2035-2045.
42. Rivière, D., Szczebara, F.M, Berjeaud, J. M., Frère, J., &, Héchard, Y., 2006. Development of a real-time PCR assay for quantification of Acanthamoeba trophozoites and cysts. J. Microbiol. Methods, 64: 78-83. (Pubitemid 41790488)
43. Rodriguez-Zaragoza, S., 1994. Ecology of free-living amoebae. Crit. Rev. Microbial., 20: 225-241. (Pubitemid 24278011)
44. Rudi, K., Moen, B., Drømtorp, S. M., &, Holck, L., 2005. Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples. Appl. Environ. Microbiol., 71: 1018-1024. (Pubitemid 40223590)
45. Simmon, K. E., Steadmana, D. D., Durkina, S., Baldwina, A., Jeffreya W., H., Sheridanb, P., Hortonb, R., &, Shieldsb, M. S., 2004. Autoclave method for rapid preparation of bacterial PCR template DNA. J. Microbiol. Methods, 56: 143-149. (Pubitemid 38117173)
46. Snealling, W. J., Moore, J. E., McKenna, J. P., Lecky, D. M., &, Dooley, J. S. G., 2006. Bacterial protozoa interactions; an update on the role these phenomena play towards human illness. Microbes Infect., 8: 578-587. (Pubitemid 43214622)
47. Soejima, T., Iida, K., Qin, T., Taniai, H., Seki, M., Takade, A., &, Yoshida, S., 2007. Photoactivated ethidium monoazide directly cleaves bacterial DNA and is applied to PCR for discrimination of live and dead bacteria. Microbiol. Immunol., 51: 763-775. (Pubitemid 47328077)
48. Steinert, M., Birkness, K., White, E., Field, B., &, Quinn, F., 1998. Mycobacterium avium bacilli grow saprophytically in coculture with Acanthamoeba polyphaga and survive within cyst walls. App. Environ. Microbiol., 64: 2256-2261. (Pubitemid 28252794)
49. Szénasi, Z., Yagita, E. K., &, Nagy, E., 1998. Isolation, identification and increasing importance of free-living ameobae causing human disease. J. Med. Microbiol., 47: 5-16. (Pubitemid 28025461)
50. Thomas, V., Bouchez, T., Nicolas, V., Robert, S., Loret, J. F., &, Lévi, Y., 2004. Amoebae in domestic water systems: resistance to disinfection treatments and implication in Legionella persistence. J. Appl. Microbiol., 97: 950-963. (Pubitemid 39390222)
51. Thompson, P. P., Kowalski, R. P., Shanks, R. M. Q., &, Gordon, Y. J., 2008. Validation of real time PCR for laboratory diagnosis of Acanthamoeba keratitis. J. Clin. Microbiol., 46: 3232-3236.
52. Tzanetou, K., Miltsakakis, D., Droutsas, D., Alimisi, S., Petropoulou, D., Ganteris, G., Dolapsaki, E., Markomichelakis, N., Mallias, I., &, Malamou-Lada, E., 2006. Acanthamoeba keratitis and contact lens disinfecting solutions. Ophthalmologica, 220: 238-241. (Pubitemid 43952572)
53. Vesper, S., McKinstry, C., Hartmann, C., Neace, M., Yoder, S., &, Vesper, A., 2008. Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J. Microbiol. Methods, 72: 180-184.
54. Wang, L., Li, Y., &, Mustapha, A., 2009. Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR. J. Appl. Microbiol., 107: 1719-1728.
55. Winiecka-Krusnell, J., Wreiber, K., von Euler, A., Engstrand, L., &, Linder, E., 2002. Free-living amoebae promote growth and survival of Helicobacter pylori. Scand. J. Infect. Dis., 34: 253-256. (Pubitemid 34520667)
56. Yañez, M. A., Nocker, A., Soria-Soria, E., Múrtula, R., Martinez, L., &, Catalán, V., 2011. Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. J. Microbiol. Methods, 85: 124-130.